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The map label for this gene is napF

Identifier: 209396222

GI number: 209396222

Start: 3077918

End: 3078412

Strand: Reverse

Name: napF

Synonym: ECH74115_3345

Alternate gene names: 209396222

Gene position: 3078412-3077918 (Counterclockwise)

Preceding gene: 209398037

Following gene: 209400362

Centisome position: 55.25

GC content: 55.15

Gene sequence:

>495_bases
GTGAAGATTGATGCATCCCGTCGGGGCATACTCACTGGTCGCTGGCGCAAAGCCAGTAACGGTATCCGTCCGCCCTGGTC
CGGTGATGAATCTCATTTTCTGACCCATTGCACCCGCTGCGACGCCTGTATTAATGCTTGTGAAAACAACATTCTGCAAC
GCGGCGCGGGCGGCTATCCGAGCGTTAATTTCAAAAATAACGAGTGCAGCTTCTGTTACGCCTGCGCGCAGGCTTGCCCC
GAATCGCTATTTTCTCCGCGCCACACCAGGGCCTGGGATTTGCAGTTCACCATTGGGGACGCGTGCCTGGCGTATCAGTC
AGTTGAATGCCGCCGCTGTCAGGATAGCTGTGAGCCAATGGCAATTATCTTTCGCCCGACGCTCTCCGGGATCTACCAGC
CGCAACTTAATAGCCAACTTTGCAACGGATGTGGAGCATGTGCAGCCAGCTGTCCGGTATCAGCCATAACTGCGGAGTAT
CTCCATGCACACTAA

Upstream 100 bases:

>100_bases
CATCCTTTAATATCTTAGCGGCTATAAAAATGGCTTATTAATTATGCGGTTTATTTGGTCGCTCTCAATTTTCAGAGCGC
GTTAATGATGGAAGGTCAAT

Downstream 100 bases:

>100_bases
CTGGCAAGTTTGCAGCCTGGTTGTGCAGGCCAAAAGCGAACGAATTTCAGACATCAGCACCCAACTGAACGCCTTTCCCG
GCTGTGAAGTTGCTGTCAGC

Product: ferredoxin-type protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYPSVNFKNNECSFCYACAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
LHAH

Sequences:

>Translated_164_residues
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYPSVNFKNNECSFCYACAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
LHAH
>Mature_164_residues
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYPSVNFKNNECSFCYACAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
LHAH

Specific function: Involved in electron transfer

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1788536, Length=164, Percent_Identity=100, Blast_Score=341, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPF_ECO57 (P0AAL2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91016
- PIR:   C85860
- RefSeq:   NP_288788.1
- RefSeq:   NP_311124.1
- ProteinModelPortal:   P0AAL2
- SMR:   P0AAL2
- EnsemblBacteria:   EBESCT00000025279
- EnsemblBacteria:   EBESCT00000060267
- GeneID:   916803
- GeneID:   958901
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3465
- KEGG:   ecs:ECs3097
- GeneTree:   EBGT00050000010390
- HOGENOM:   HBG492386
- OMA:   NALRPPW
- ProtClustDB:   PRK10194
- BioCyc:   ECOL83334:ECS3097-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496
- TIGRFAMs:   TIGR00402

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 18048; Mature: 18048

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
11.0 %Cys+Met (Translated Protein)
9.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
11.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
AIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEYLHAH
EEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGAGGYP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
AIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEYLHAH
EEEEECCHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796