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The map label for this gene is nuoM

Identifier: 209396119

GI number: 209396119

Start: 3158977

End: 3160506

Strand: Reverse

Name: nuoM

Synonym: ECH74115_3416

Alternate gene names: 209396119

Gene position: 3160506-3158977 (Counterclockwise)

Preceding gene: 209398279

Following gene: 209396747

Centisome position: 56.72

GC content: 55.03

Gene sequence:

>1530_bases
ATGTTATTACCCTGGTTAATACTAATCCCCTTTATCGGCGGCTTCCTGTGCTGGCAGACCGAACGCTTTGGCGTCAAGGT
GCCGCGCTGGATCGCGCTGATCACCATGGGATTGACGCTGGCGCTGTCGCTGCAACTGTGGTTGCAGGGCGGTTATTCAC
TGACGCAATCCGCCGGAATTCCGCAGTGGCAGTCTGAATTCGACATGCCGTGGATCCCGCGTTTTGGTATCTCTATCCAT
CTCGCCATTGACGGGCTGTCGCTGCTGATGGTCGTGCTGACCGGTCTGCTCGGTGTGCTGGCGGTACTCTGTTCGTGGAA
AGAGATCGAAAAATATCAGGGCTTCTTCCACCTCAACCTGATGTGGATCCTGGGCGGCGTTATCGGCGTGTTCCTTGCCA
TCGACATGTTCCTGTTCTTCTTCTTCTGGGAAATGATGCTGGTGCCGATGTACTTCCTGATCGCACTGTGGGGGCATAAA
GCCTCTGATGGTAAAACGCGTATCACGGCGGCAACCAAGTTCTTCATTTACACCCAGGCGAGTGGTCTGGTGATGTTGAT
CGCCATCCTGGCGCTGGTTTTTGTTCACTACAATGCGACCGGCGTCTGGACCTTCAACTATGAAGAGCTGCTGAATACGC
CAATGTCCAGTGGTGTGGAATATCTGTTGATGCTGGGCTTCTTCATCGCGTTTGCGGTGAAAATGCCGGTGGTTCCGCTG
CATGGCTGGCTGCCGGATGCGCACTCACAGGCACCGACCGCCGGTTCCGTGGACCTCGCGGGGATCTTGCTGAAAACCGC
CGCTTACGGTCTGCTGCGTTTCTCCCTACCGCTGTTCCCAAACGCGTCGGCAGAGTTCGCGCCAATCGCCATGTGGCTGG
GTGTTATCGGCATCTTCTACGGTGCGTGGATGGCCTTCGCCCAGACCGATATCAAACGTCTGATCGCCTACACCTCGGTT
TCCCACATGGGCTTCGTACTGATTGCTATCTACACCGGCAGCCAGTTGGCCTACCAGGGCGCGGTAATCCAGATGATTGC
GCACGGCTTGTCAGCGGCGGGTCTGTTTATTCTTTGTGGTCAGCTTTATGAACGTATCCATACCCGCGACATGCGCATGA
TGGGCGGTCTGTGGAGCAAGATGAAATGGCTGCCAGCCTTGTCACTGTTCTTTGCGGTGGCAACGCTCGGGATGCCAGGC
ACCGGTAACTTCGTCGGCGAATTTATGATTCTGTTCGGCAGCTTCCAGGTTGTCCCGGTGATTACCGTTATCTCTACCTT
TGGGCTGGTCTTTGCATCTGTTTATTCGCTGGCGATGTTACATCGCGCTTACTTCGGTAAAGCGAAAAGCCAGATTGCCA
GCCAGGAACTGCCAGGGATGTCGCTGCGTGAGCTGTTTATGATCCTGTTGCTGGTGGTGCTGCTGGTACTGCTGGGCTTC
TATCCGCAGCCGATTCTGGATACCTCGCACTCCGCGATTGGCAATATCCAGCAGTGGTTTGTTAATTCCGTTACTACTAC
AAGGCCGTAA

Upstream 100 bases:

>100_bases
GGAGCGTACTTAAGTACGTGAGGATTGCGAGCACTGCCGGGATTCGGGCTGGCGAGTGAGATAGCCTGAGCAGGAGTAAA
TATAAGGGACATACTTAGCT

Downstream 100 bases:

>100_bases
ATCGCCATGACAATAACTCCACAAAACCTGATCGCACTGCTACCGTTGCTGATCGTCGGCTTGACGGTGGTGGTTGTGAT
GCTCTCCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIH
LAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP

Sequences:

>Translated_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIH
LAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP
>Mature_509_residues
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIH
LAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGF
YPQPILDTSHSAIGNIQQWFVNSVTTTRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=33.4239130434783, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI251831117, Length=328, Percent_Identity=27.1341463414634, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788613, Length=509, Percent_Identity=100, Blast_Score=1014, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=452, Percent_Identity=25.4424778761062, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788831, Length=499, Percent_Identity=26.0521042084168, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788614, Length=494, Percent_Identity=25.9109311740891, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693160, Length=436, Percent_Identity=25.2293577981651, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788829, Length=234, Percent_Identity=26.4957264957265, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOM_ECO57 (P0AFE9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91024
- PIR:   B85868
- RefSeq:   NP_288851.1
- RefSeq:   NP_311188.1
- ProteinModelPortal:   P0AFE9
- EnsemblBacteria:   EBESCT00000027994
- EnsemblBacteria:   EBESCT00000059587
- GeneID:   916869
- GeneID:   957330
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3536
- KEGG:   ecs:ECs3161
- GeneTree:   EBGT00050000009194
- HOGENOM:   HBG643593
- OMA:   GVLTFNY
- ProtClustDB:   PRK05846
- BioCyc:   ECOL83334:ECS3161-MONOMER
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- PRINTS:   PR01437
- TIGRFAMs:   TIGR01972

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 56525; Mature: 56525

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xffeeb4c)-; HASH(0xf6530f8)-; HASH(0xf6f52e4)-; HASH(0xef39d48)-; HASH(0xfb2d7d4)-; HASH(0xf6336fc)-; HASH(0xf920138)-; HASH(0xf1fa5ac)-; HASH(0x102a305c)-; HASH(0xff40cd4)-; HASH(0xf8fb9e0)-; HASH(0xfce5270)-; HASH(0x10376220)-; HASH(0xfe5cbf0)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNL
CCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAIGNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSLQLWLQGGYSLTQSAGI
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
PQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAVLCSWKEIEKYQGFFHLNL
CCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
MWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHH
ITVISTFGLVFASVYSLAMLHRAYFGKAKSQIASQELPGMSLRELFMILLLVVLLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
YPQPILDTSHSAIGNIQQWFVNSVTTTRP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796