Click here to switch to the map view.

The map label for this gene is hemN [C]

Identifier: 209395960

GI number: 209395960

Start: 4496140

End: 4497477

Strand: Direct

Name: hemN [C]

Synonym: ECH74115_4862

Alternate gene names: 209395960

Gene position: 4496140-4497477 (Clockwise)

Preceding gene: 209396547

Following gene: 209397453

Centisome position: 80.69

GC content: 51.35

Gene sequence:

>1338_bases
ATGAACGCAAATAACACCCTGGATCTCACGCCACATTTTGCTTTAGACGGTGATCAGCCATTTAAAGACAGGCGCGCCAT
GATGCCGTTTCGTGGAGCCATTCCGGTTGCCAAAGAGCAACTGGCACAGACCTGGCAAGAGATGATCAATCAAACGGCAT
CACCTCGTAAGCGACTGGTTTACCTACACATTCCTTTTTGTGCGACACACTGCACGTTTTGTGGTTTTTATCAGAACCGT
TTTAATGAAGATGCGTGTGCTCATTATACCGACGCCCTGATTCGTGAAATAGAAATGGAAGCGGACAGCGTATTGCATCA
GTCTGCCCCCATCCACGCGGTCTATTTCGGTGGCGGTACGCCTTCCGCACTTTCGGCACACGATTTGGCCAGAATCATCA
CCACATTACGAGAAAAGCTGCCACTGGCCCCTGATTGTGAAATCACTATCGAAGGACGGGTATTGAATTTTGACGCTGAG
CGAATCGATGCTTGCCTTGATGCAGGAGCAAACCGCTTCTCAATTGGTATCCAGTCGTTTAACAGCAAAATCCGCAAGAA
AATGGCCCGCACCTCGGATGGCCCAACCGCTATTGCGTTTATGGAAAGCCTGGTCAAACGTGACCGTGCTGCGGTGGTCT
GTGACCTGCTGTTTGGCCTGCCGGGTCAGGATGCGCAAACCTGGGGAGAAGATCTGGCTATTGCCCGCGATATCGGTCTC
GACGGCGTCGATCTCTATGCGCTCAATGTCCTGTCCAATACACCGCTGGGCAAGGCCGTGGAAAATGGGCGTACTACCGT
ACCCTCACCGGCAGAACGTCGCGATCTTTATCTGCAAGGATGTGATTTTATGGACGATGCCGGTTGGCGCTGCATCAGTA
ACAGCCACTGGGGCCGTACCACACGCGAACGCAATCTCTATAACCTGCTGATTAAACAAGGTGCCGATTGTCTGGCCTTT
GGTTCCGGAGCCGGCGGTTCGATTAATGGTTACTCCTGGATGAACGAACGCAATCTTCAGACCTGGCATGAATCCGTCGC
GGCAGGCAAAAAACCGCTGATGTTGATCATGCGTAACGCCGAACGTAATGCGCAATGGCGTCATACCTTGCAGTCAGGTG
TTGAAACAGCGCGCGTACCGCTGGATGAACTTACGCCACATGCCGAAAAACTCGCGCCGTTACTGGCTCAATGGCACCAA
AAAGGCTTAAGTCGCGATGCTTCAACTTGCCTGCGGCTGACTAATGAAGGTCGTTTCTGGGCAAGCAATATTTTGCAGTC
TCTTAATGAACTAATTCAGGTACTTAATGCGCCAGCGATTATGCGTGAAAAACCATAA

Upstream 100 bases:

>100_bases
GATCAAAACCTGATTCTGGGAATGGGTCCGCGTATTGCTGATGTCGTTGAGTCTTTACACCAGCAGCTTTGGCCGCAATA
AATGGATTTAAATCAGACGA

Downstream 100 bases:

>100_bases
CAGGAGTGATTTATGAGCCATGTCTCGTTACAAGAATTTTTGAAAACGGAGCCGGACGGTACGCTGGAGGTGGTAGCCGA
ACAATACAACACCACACTGC

Product: coproporphyrinogen III oxidase

Products: Protoporphyrinogen IX; CO2; H2O

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQTWQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNR
FNEDACAHYTDALIREIEMEADSVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLFGLPGQDAQTWGEDLAIARDIGL
DGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDLYLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAF
GSGAGGSINGYSWMNERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLAPLLAQWHQ
KGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP

Sequences:

>Translated_445_residues
MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQTWQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNR
FNEDACAHYTDALIREIEMEADSVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLFGLPGQDAQTWGEDLAIARDIGL
DGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDLYLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAF
GSGAGGSINGYSWMNERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLAPLLAQWHQ
KGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP
>Mature_445_residues
MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQTWQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNR
FNEDACAHYTDALIREIEMEADSVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLFGLPGQDAQTWGEDLAIARDIGL
DGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDLYLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAF
GSGAGGSINGYSWMNERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLAPLLAQWHQ
KGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP

Specific function: Anaerobic Transformation Of Coproporphyrinogen-III Into Protoporphyrinogen-IX. [C]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=275, Percent_Identity=28.3636363636364, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI87082341, Length=322, Percent_Identity=24.8447204968944, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789325, Length=275, Percent_Identity=28.3636363636364, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: 1.3.3.3

Molecular weight: Translated: 49550; Mature: 49550

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQTWQEMINQTASPRKRLV
CCCCCCEECCCCEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
YLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEADSVLHQSAPIHAVYFGGGT
EEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
PSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAERIDACLDAGANRFSIGIQSF
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECEEEECCHHHHHHHHHCCCCEEEHHHHHH
NSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLFGLPGQDAQTWGEDLAIARDIGL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
DGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDLYLQGCDFMDDAGWRCISNSHWGRT
CCHHHEEEHHHCCCCCCHHHHCCCCCCCCCHHHCCEEEECCCCCCCCCCEEECCCCCCCC
TRERNLYNLLIKQGADCLAFGSGAGGSINGYSWMNERNLQTWHESVAAGKKPLMLIMRNA
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECC
ERNAQWRHTLQSGVETARVPLDELTPHAEKLAPLLAQWHQKGLSRDASTCLRLTNEGRFW
CCCHHHHHHHHHCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHH
ASNILQSLNELIQVLNAPAIMREKP
HHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQTWQEMINQTASPRKRLV
CCCCCCEECCCCEECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEE
YLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEADSVLHQSAPIHAVYFGGGT
EEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
PSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAERIDACLDAGANRFSIGIQSF
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECEEEECCHHHHHHHHHCCCCEEEHHHHHH
NSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLFGLPGQDAQTWGEDLAIARDIGL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
DGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDLYLQGCDFMDDAGWRCISNSHWGRT
CCHHHEEEHHHCCCCCCHHHHCCCCCCCCCHHHCCEEEECCCCCCCCCCEEECCCCCCCC
TRERNLYNLLIKQGADCLAFGSGAGGSINGYSWMNERNLQTWHESVAAGKKPLMLIMRNA
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECC
ERNAQWRHTLQSGVETARVPLDELTPHAEKLAPLLAQWHQKGLSRDASTCLRLTNEGRFW
CCCHHHHHHHHHCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHH
ASNILQSLNELIQVLNAPAIMREKP
HHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen III; O2; H+

Specific reaction: coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]