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The map label for this gene is trmE [H]

Identifier: 209395938

GI number: 209395938

Start: 4776165

End: 4777529

Strand: Direct

Name: trmE [H]

Synonym: ECH74115_5136

Alternate gene names: 209395938

Gene position: 4776165-4777529 (Clockwise)

Preceding gene: 209400160

Following gene: 209397514

Centisome position: 85.72

GC content: 56.48

Gene sequence:

>1365_bases
ATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGCTT
CAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACG
CCGATGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGGCCCGAACTCGTTCACCGGCGAAGATGTGCTGGAA
CTGCAGGGTCATGGCGGTCCGGTGATTCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCG
CCCTGGTGAGTTTTCCGAACGCGCGTTTCTTAACGATAAACTCGACTTAGCCCAGGCCGAGGCGATTGCCGATCTTATCG
ATGCCAGTTCGGAACAGGCAGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTCAACCATCTGGTG
GAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCCGATGAAGAGATCGATTTCCTCTCCGACGG
AAAAATTGAAGCCCAGCTTAATGACGTGATTGCCGATCTCGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC
GCGAAGGGATGAAGGTGGTGATTGCCGGACGTCCTAATGCCGGTAAATCGAGCCTGTTAAACGCGCTGGCTGGCCGTGAA
GCAGCAATCGTAACCGATATCGCCGGAACCACGCGTGACGTGTTGCGTGAGCATATCCACATTGACGGAATGCCGCTGCA
TATCATCGACACCGCCGGGCTACGTGAAGCCAGCGATGAAGTGGAACGTATTGGTATCGAGCGCGCGTGGCAAGAAATTG
AACAAGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACGACAGACGCCGTTGATCCGGCGGAGATCTGGCCGGAATTT
ATCGCCCGTCTGCCCGCGAAACTGCCGATCACCGTGGTACGCAATAAAGCCGATATCACTGGCGAAACGCTGGGAATGAG
TGAAGTGAACGGTCATGCGTTGATTCGTCTCTCGGCAAAGACTGGCGAAGGCGTGGACGTGCTGCGTAACCATCTCAAAC
AGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTTCAGGCGCTGGAACAGGCAGCG
GAGCATTTGCAGCAAGGTAAAGCGCAACTGTTGGGTGCCTGGGCGGGCGAACTGCTGGCGGAAGAGTTGCGCCTGGCACA
GCAGAACTTAAGCGAAATCACCGGGGAATTTACCTCTGATGATTTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTA
AGTAA

Upstream 100 bases:

>100_bases
TGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGTATCCGCCA
CACAAAGCAACAGGAACATC

Downstream 100 bases:

>100_bases
GTCGTCTAAATTGATATAAAGGGCACTGTTCCGTTCTGGGGGAAACTAACTATCAGAACGGAACAGCGCTTGTTTTTTTA
TGAGCATCCTGCACCAAAAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEF
IARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAA
EHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEF
IARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAA
EHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK
>Mature_453_residues
SDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFI
ARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAAE
HLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=36.4025695931477, Blast_Score=252, Evalue=6e-67,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=35, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=35.5748373101952, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=99.7797356828194, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=40.6593406593407, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=28.0434782608696, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.6288032454361, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI45550813, Length=485, Percent_Identity=32.9896907216495, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49203; Mature: 49072

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLD
CCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAK
EEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECC
TGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAAEHLQQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLD
CCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAK
EEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECC
TGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAAEHLQQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA