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The map label for this gene is mdtL [H]

Identifier: 209395703

GI number: 209395703

Start: 4783419

End: 4784594

Strand: Direct

Name: mdtL [H]

Synonym: ECH74115_5141

Alternate gene names: 209395703

Gene position: 4783419-4784594 (Clockwise)

Preceding gene: 209399599

Following gene: 209397765

Centisome position: 85.85

GC content: 52.38

Gene sequence:

>1176_bases
ATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACTTTATCCCGCTGGGATTGATATGTACCTCGTTGGTTTACC
GCGCATCGCCGCCGATCTCAATGCCAGCGAAGCGCAGTTACATATTGCGTTCTCCGTTTATCTGGCAGGTATGGCAGCTG
CGATGTTATTTGCCGGTAAAGTGGCCGATCGTTCAGGGAGAAAACCGGTCGCCATCCCCGGCGCGGCGCTATTTATTATC
ACTTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGGCGCTTTCTACAGGGGTTGGGCGCGGGCTG
TTGTTACGTGGTGGCATTCGCCATTTTGCGCGACACGCTGGATGATCGACGTCGGGCAAAAGTGCTGTCGTTACTCAACG
GCATTACCTGCATCATTCCGGTGTTAGCGCCGGTGCTCGGTCATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTC
TGGACGATGGCAATAATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCTGCCGCCCCCGC
AGCTTCGGACAAATCACGAGAAAATAGCGAGTCGCTGCTTAATCGGTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCA
GCGTTTCGGTGATCCTCACCTTCGTCAATACATCGCCGGTATTGCTGATGGAAATCATGGGTTTTGAGCGCGGAGAATAC
GCCACCATTATGGCGTTGACCGCTGGCGTCAGCATGACCGTTTCATTTTCCACGCCATTTGCGCTGGGAATTTTTAAGCC
ACGTACGTTGATGATCACCTCACAGGTGTTATTCCTGGCGGCGGGCATCACTCTTACCGTTTCACCTTCCCATGCGGTTT
CTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTT
TCATTACGCGCGGGCGTAGCCAGCTCGACCTTAGGTATTGCGCAGGTTTGCGGTTCGTCACTGTGGATTTGGCTGGCAGC
GGTGGTTGGTATCAGCGCATGGAATATGCTGATCGGGATTCTGATTGCCTGTAGCATAGTGAGCCTGTTGCTGATTATGT
TCGTCGCGCCTGGACGCCCGGTTACCGCTCATGAAGAAATCCATCACCACGCTTGA

Upstream 100 bases:

>100_bases
CTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTA
CTATCAGGACGCTTTAGCCC

Downstream 100 bases:

>100_bases
TCTCAATCTGCTGCTCTGTCTGCAGCTGCTGATGCAGGAGCGCAGCGTAACCAAAGCGGCGAAGCGGATGAACGTGACAC
CTTCGGCGGTGAGTAAGTCG

Product: multidrug efflux system protein MdtL

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
TSVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA

Sequences:

>Translated_391_residues
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFII
TSVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPF
SLRAGVASSTLGIAQVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA
>Mature_390_residues
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIT
SVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFW
TMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEYA
TIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFS
LRAGVASSTLGIAQVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=391, Percent_Identity=98.2097186700767, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1788509, Length=226, Percent_Identity=30.5309734513274, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI48994889, Length=242, Percent_Identity=29.7520661157025, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790794, Length=227, Percent_Identity=28.1938325991189, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87082312, Length=338, Percent_Identity=25.7396449704142, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787065, Length=233, Percent_Identity=23.6051502145923, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081738, Length=148, Percent_Identity=31.7567567567568, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41612; Mature: 41481

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWTMAIMGIAVLMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLTVSPSHAVSLFGITL
HEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGISAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LIACSIVSLLLIMFVAPGRPVTAHEEIHHHA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
CCHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHH
VADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
HHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWTMAIMGIAVLMLSLFILKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
TRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCH
ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLTVSPSHAVSLFGITL
HEEEEEECCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHH
ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGISAWNMLIGI
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LIACSIVSLLLIMFVAPGRPVTAHEEIHHHA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA