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The map label for this gene is rpoS [H]

Identifier: 209695924

GI number: 209695924

Start: 2686176

End: 2687141

Strand: Reverse

Name: rpoS [H]

Synonym: VSAL_I2506

Alternate gene names: 209695924

Gene position: 2687141-2686176 (Counterclockwise)

Preceding gene: 209695925

Following gene: 209695919

Centisome position: 80.81

GC content: 39.75

Gene sequence:

>966_bases
ATGAGTAAAAGCAATGCAGTAACTAAAGTTGAAGAATTTGATTTAGACGTAGTTGATGAAACTGAACTAACGACAAAGAC
AGAAAAAAAATCTGAACGTCTTGAAGTTAGTAGTTCGCAAAAAGTAATGGATGCGACTCAACTGTACTTAAGTGAAATTG
GATTTTCACCACTTCTAACCGCTGAAGAGGAAGTGTATTTCAGTCGCCTAGCCTTGAAAGGTGATGTTGCCGCTCGATCT
AGAATGATTGAAAGTAACTTACGATTAGTGGTGAAAATTGCACGTCGTTATAACCATCGTGGTCTTGCTTTATTAGATTT
GGTTGAAGAAGGTAATTTAGGTTTAATTCGCGCGGTTGAAAAATTTGATCCAGAAAGAGGCTTTCGCTTCTCTACTTATG
CCACTTGGTGGATACGTCAAACGATTGAACGTGCCATTATGAACCAAACGAGAACCATTCGTTTACCTATCCACGTAGTG
AAAGAGTTAAACGTATACCTGCGTGCCGCTCGTGAGCTATCCCAAAAATTGGATCATGAACCTACACCAGAAGAAATAGC
AGAAAAGCTAGATAAACCAGTAAGCGATGTGAGTCGAATGCTGCGTTTAAACGAGAGGGTCGCTTCTGTTGATTCAATGG
TTGCAGGTGACTCAGATAAAGCGCTGCTTGATATTATTCCGGATACGAAACATGCCGATCCTGAATCCGAAACACAAACA
AACGATATTAACGAATCTTTAGTTGGCTGGTTAGGTCAATTAAATCCAAAACAAAAAGAAGTCGTTGCACGTCGTTTTGG
ACTGTTAGGGTATGAAGCGTCAACGTTAGAACAGGTAGGCCGAGAGATTGGTTTAACTCGAGAACGAGTAAGACAAATCC
AAGTTGAAGGGTTAAGGCGTTTAAGAGAAATAATGACGCAACAAGGTTTGAATATGGAAACTCTATTTGCTGTTGATGAC
TTTTAA

Upstream 100 bases:

>100_bases
CGTTTTAGAGGGAAATCGGTAAATCCACAACGGTATTTACCGTAACAGGGATGACATAGCTGAGTTGTAATGTCTTGCTG
GCTCGCCATAGGGAGGCGCT

Downstream 100 bases:

>100_bases
ACTCATTTTTATTTAACAATAAGTAGAATCATAAAAGCCACAGCAAGGTTACTTGCGGTGGCTTTTTTAGTTTATGAGCA
AGAGGATTGATTTATCATTG

Product: RNA polymerase sigma subunit RpoS (sigma-38)

Products: NA

Alternate protein names: Sigma-38 factor [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKSNAVTKVEEFDLDVVDETELTTKTEKKSERLEVSSSQKVMDATQLYLSEIGFSPLLTAEEEVYFSRLALKGDVAARS
RMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVV
KELNVYLRAARELSQKLDHEPTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGDSDKALLDIIPDTKHADPESETQT
NDINESLVGWLGQLNPKQKEVVARRFGLLGYEASTLEQVGREIGLTRERVRQIQVEGLRRLREIMTQQGLNMETLFAVDD
F

Sequences:

>Translated_321_residues
MSKSNAVTKVEEFDLDVVDETELTTKTEKKSERLEVSSSQKVMDATQLYLSEIGFSPLLTAEEEVYFSRLALKGDVAARS
RMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVV
KELNVYLRAARELSQKLDHEPTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGDSDKALLDIIPDTKHADPESETQT
NDINESLVGWLGQLNPKQKEVVARRFGLLGYEASTLEQVGREIGLTRERVRQIQVEGLRRLREIMTQQGLNMETLFAVDD
F
>Mature_320_residues
SKSNAVTKVEEFDLDVVDETELTTKTEKKSERLEVSSSQKVMDATQLYLSEIGFSPLLTAEEEVYFSRLALKGDVAARSR
MIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVK
ELNVYLRAARELSQKLDHEPTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGDSDKALLDIIPDTKHADPESETQTN
DINESLVGWLGQLNPKQKEVVARRFGLLGYEASTLEQVGREIGLTRERVRQIQVEGLRRLREIMTQQGLNMETLFAVDDF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. May be required for the persistence of V.cholerae in aquatic habitats [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=307, Percent_Identity=75.5700325732899, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=42.2222222222222, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789871, Length=274, Percent_Identity=27.3722627737226, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36674; Mature: 36543

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSNAVTKVEEFDLDVVDETELTTKTEKKSERLEVSSSQKVMDATQLYLSEIGFSPLLT
CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AEEEVYFSRLALKGDVAARSRMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARELSQKLDHE
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGDSDKALLDIIPDTKHADPESETQT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCC
NDINESLVGWLGQLNPKQKEVVARRFGLLGYEASTLEQVGREIGLTRERVRQIQVEGLRR
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LREIMTQQGLNMETLFAVDDF
HHHHHHHCCCCEEEEEEECCC
>Mature Secondary Structure 
SKSNAVTKVEEFDLDVVDETELTTKTEKKSERLEVSSSQKVMDATQLYLSEIGFSPLLT
CCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AEEEVYFSRLALKGDVAARSRMIESNLRLVVKIARRYNHRGLALLDLVEEGNLGLIRAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARELSQKLDHE
HCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
PTPEEIAEKLDKPVSDVSRMLRLNERVASVDSMVAGDSDKALLDIIPDTKHADPESETQT
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCC
NDINESLVGWLGQLNPKQKEVVARRFGLLGYEASTLEQVGREIGLTRERVRQIQVEGLRR
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LREIMTQQGLNMETLFAVDDF
HHHHHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473029; 10952301 [H]