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The map label for this gene is ecfE [C]

Identifier: 209695844

GI number: 209695844

Start: 2590443

End: 2591801

Strand: Reverse

Name: ecfE [C]

Synonym: VSAL_I2420

Alternate gene names: 209695844

Gene position: 2591801-2590443 (Counterclockwise)

Preceding gene: 209695845

Following gene: 209695843

Centisome position: 77.95

GC content: 38.78

Gene sequence:

>1359_bases
ATGTCAGAACTACTTTGGAATTTAGCATCGTTTATCATTGCTCTCGGTATTTTGGTTGCCGTGCATGAGTATGGCCATTT
CTGGGTAGCTCGCCGTTGTGGCGTTATTGTTGAAAAATTCTCTATTGGATTTGGTAAATCTATTTGGAGTAGAAAAGGCA
AAGACGGTACAGAATACAGCATTTCAATGATCCCTTTAGGTGGATATGTGAAGATGCTGGATGAGCGAGTCGATGACGTC
CCTGAAGAATTAAAAAAACACGCATTTAATAATCGTCCGTTATGGCAACGAAGTGCAATTGTTGCTGCGGGTCCTATTGC
TAACTTTATTTTTGCCGTTTTTGCTTGTTGGTTAGCTTTCATGATCGGGGTGACTGCATTAAAGCCAGTAGTTGGTTCTG
TTGAAAACAACTCTATATTTTCTCAAGCAGGAATTGAATCTGGAGTGGAACTAAAAGCCATTTCAGGAATCAAAACCTCG
GATTGGGAATCGGTTAATATGGCGATTGTTTCTCATATTGGTGATGAATCAATGACGGTAACTTATAGTGACAGCGATAA
TATTGGTGTTGATGTAACTAAGCGTTTGGATCTATCGTCATGGAAATTCGATCCTGAAAAAGATTCACCAATGCTAACGT
TGGGTTTTAGACCTTACCGACCAGAAGTAACATTAACGATAGCTAATGTTAGTGATAATAGTGCCGCGAGTCGTTCTGGA
ATGTTAATTGGGGATATTCTTTCTTCAGTGAACGGTAAAGAATTATCCAAATGGCAGCAAATGGTGGATGAAATTCAAGG
CAATCCTAGTGCGCCAATGTCAATTGTTGTTATTCGTGATGGCATTGATACGACATTGACGTTAACACCAGATAGCCGCG
AGGATTCGGAAGGTAAATTAATCGGTTTTGCTGGTGTTTCACCTGAGTTTAAAGGGTGGCCTGAAGGATATCGTTATGAA
AAACAATATGGTCCAATTGTTGCGTTAGAAAAAGCAATGATAAAAACTGGCAATATTATAGATTTAACATTAACCATGAC
TAAGAAGTTATTCACGGGTGATGTCGCTTTAAACAACCTAAGTGGTCCTATTTCTATAGCAAAAGGAGCAGGGGCCACCG
CGGATTATGGTATTGTTTCATTTTTAGGATTTTTAGCCTTAATTAGCGTAAATTTAGGTATTATCAATTTATTACCTCTT
CCGGTATTGGATGGCGGTCATTTACTGTTTTTTGCTATCGAGGGAATTACACGTAAACCCGTACCAGAAAGAGTTCAAGA
AATCGGTTATAAAGTAGGAACAGCACTTATTATGTCTTTAATGGCTGTTGCATTATTTAACGACTTTATGCGCCTTTAA

Upstream 100 bases:

>100_bases
CAAACTTCCAAAACCTTGAGCTAGATAGCTTGGAAAGCCTGATCGACCTAGATAGAATGGCGCGTAATTATGCCGATGAG
GCTCTTAAAAAGGTTTAACT

Downstream 100 bases:

>100_bases
GCAGTAGCAAGGAATAATTAGACGTATTATGGCGATGAAAAAATTGTTGATTGCTACATTGCTTTTTAGCAGTGTAGCAG
CACAAAGCGCAGAAACATTT

Product: protease EcfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSELLWNLASFIIALGILVAVHEYGHFWVARRCGVIVEKFSIGFGKSIWSRKGKDGTEYSISMIPLGGYVKMLDERVDDV
PEELKKHAFNNRPLWQRSAIVAAGPIANFIFAVFACWLAFMIGVTALKPVVGSVENNSIFSQAGIESGVELKAISGIKTS
DWESVNMAIVSHIGDESMTVTYSDSDNIGVDVTKRLDLSSWKFDPEKDSPMLTLGFRPYRPEVTLTIANVSDNSAASRSG
MLIGDILSSVNGKELSKWQQMVDEIQGNPSAPMSIVVIRDGIDTTLTLTPDSREDSEGKLIGFAGVSPEFKGWPEGYRYE
KQYGPIVALEKAMIKTGNIIDLTLTMTKKLFTGDVALNNLSGPISIAKGAGATADYGIVSFLGFLALISVNLGIINLLPL
PVLDGGHLLFFAIEGITRKPVPERVQEIGYKVGTALIMSLMAVALFNDFMRL

Sequences:

>Translated_452_residues
MSELLWNLASFIIALGILVAVHEYGHFWVARRCGVIVEKFSIGFGKSIWSRKGKDGTEYSISMIPLGGYVKMLDERVDDV
PEELKKHAFNNRPLWQRSAIVAAGPIANFIFAVFACWLAFMIGVTALKPVVGSVENNSIFSQAGIESGVELKAISGIKTS
DWESVNMAIVSHIGDESMTVTYSDSDNIGVDVTKRLDLSSWKFDPEKDSPMLTLGFRPYRPEVTLTIANVSDNSAASRSG
MLIGDILSSVNGKELSKWQQMVDEIQGNPSAPMSIVVIRDGIDTTLTLTPDSREDSEGKLIGFAGVSPEFKGWPEGYRYE
KQYGPIVALEKAMIKTGNIIDLTLTMTKKLFTGDVALNNLSGPISIAKGAGATADYGIVSFLGFLALISVNLGIINLLPL
PVLDGGHLLFFAIEGITRKPVPERVQEIGYKVGTALIMSLMAVALFNDFMRL
>Mature_451_residues
SELLWNLASFIIALGILVAVHEYGHFWVARRCGVIVEKFSIGFGKSIWSRKGKDGTEYSISMIPLGGYVKMLDERVDDVP
EELKKHAFNNRPLWQRSAIVAAGPIANFIFAVFACWLAFMIGVTALKPVVGSVENNSIFSQAGIESGVELKAISGIKTSD
WESVNMAIVSHIGDESMTVTYSDSDNIGVDVTKRLDLSSWKFDPEKDSPMLTLGFRPYRPEVTLTIANVSDNSAASRSGM
LIGDILSSVNGKELSKWQQMVDEIQGNPSAPMSIVVIRDGIDTTLTLTPDSREDSEGKLIGFAGVSPEFKGWPEGYRYEK
QYGPIVALEKAMIKTGNIIDLTLTMTKKLFTGDVALNNLSGPISIAKGAGATADYGIVSFLGFLALISVNLGIINLLPLP
VLDGGHLLFFAIEGITRKPVPERVQEIGYKVGTALIMSLMAVALFNDFMRL

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=454, Percent_Identity=54.625550660793, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 49199; Mature: 49067

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLWNLASFIIALGILVAVHEYGHFWVARRCGVIVEKFSIGFGKSIWSRKGKDGTEYS
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEE
ISMIPLGGYVKMLDERVDDVPEELKKHAFNNRPLWQRSAIVAAGPIANFIFAVFACWLAF
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEEECCHHHHHHHHHHHHHHHH
MIGVTALKPVVGSVENNSIFSQAGIESGVELKAISGIKTSDWESVNMAIVSHIGDESMTV
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEE
TYSDSDNIGVDVTKRLDLSSWKFDPEKDSPMLTLGFRPYRPEVTLTIANVSDNSAASRSG
EECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCC
MLIGDILSSVNGKELSKWQQMVDEIQGNPSAPMSIVVIRDGIDTTLTLTPDSREDSEGKL
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCE
IGFAGVSPEFKGWPEGYRYEKQYGPIVALEKAMIKTGNIIDLTLTMTKKLFTGDVALNNL
EEEECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCCEEEEEEEEHHHHHCCCEEECCC
SGPISIAKGAGATADYGIVSFLGFLALISVNLGIINLLPLPVLDGGHLLFFAIEGITRKP
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCEEEEEEECCCCCCC
VPERVQEIGYKVGTALIMSLMAVALFNDFMRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELLWNLASFIIALGILVAVHEYGHFWVARRCGVIVEKFSIGFGKSIWSRKGKDGTEYS
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEE
ISMIPLGGYVKMLDERVDDVPEELKKHAFNNRPLWQRSAIVAAGPIANFIFAVFACWLAF
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEEECCHHHHHHHHHHHHHHHH
MIGVTALKPVVGSVENNSIFSQAGIESGVELKAISGIKTSDWESVNMAIVSHIGDESMTV
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEE
TYSDSDNIGVDVTKRLDLSSWKFDPEKDSPMLTLGFRPYRPEVTLTIANVSDNSAASRSG
EECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCC
MLIGDILSSVNGKELSKWQQMVDEIQGNPSAPMSIVVIRDGIDTTLTLTPDSREDSEGKL
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCE
IGFAGVSPEFKGWPEGYRYEKQYGPIVALEKAMIKTGNIIDLTLTMTKKLFTGDVALNNL
EEEECCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCCEEEEEEEEHHHHHCCCEEECCC
SGPISIAKGAGATADYGIVSFLGFLALISVNLGIINLLPLPVLDGGHLLFFAIEGITRKP
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCEEEEEEECCCCCCC
VPERVQEIGYKVGTALIMSLMAVALFNDFMRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]