Click here to switch to the map view.

The map label for this gene is dusC [H]

Identifier: 209695593

GI number: 209695593

Start: 2309269

End: 2310216

Strand: Direct

Name: dusC [H]

Synonym: VSAL_I2152

Alternate gene names: 209695593

Gene position: 2309269-2310216 (Clockwise)

Preceding gene: 209695592

Following gene: 209695598

Centisome position: 69.45

GC content: 39.66

Gene sequence:

>948_bases
ATGCAAGTAATACTAGGCCCTATGGAGGGCGTACTCGATCACTTAATGCGTGAACTATTAACAGAAATTAACGACTATGA
TTTCTGTGTTACTGAGTTTGTGCGCATCGTTGATCAACTTTTACCTCCTCATGTATTCCACCGAATCTGTCCGGAATTAC
ATAACGACAGTAAAACCAAATCAGGTACCCCTATTCGTCTACAATTATTAGGACAAGAACCTAATTGGATGGCAGAAAAC
GCGGCACGAGCAATTGATCTTGGTTCTGATGGTCTTGATTTAAACTTTGGTTGCCCAGCAAAAGCGGTAAACAAAAGTCG
GGGCGGAGCTGCGCTTTTAAAAGATCCTGAATTAATTTATCAAATCGTTAAAGCGTGTCGTGAAGCAGTACCACAAGATA
AAAAAGTCACTGCAAAAATTCGCTTAGGTTGGGAGAATCCCGAAGAATGTTTTGCTATTGCCGATGCGATAAAACAAGCT
GGCGCAAACGAATTAACCATCCATGCGCGCACTAAAACTGATGGCTATCGTGCAGAAGAAATAAAATGGGATTACATCAA
CCAAGTTCGCCAAAAAATAGATATTCCTATTATCGCTAATGGTGAAATTTGGAACTATGCCGATGGACAACGTTGTATTG
CCGCCACAGGAATTGATTCGTTAATGGTGTGCCGAGGCGCATTTAATATGCCAAACCTTGGGAACGTAGTGAAACACAAT
CACCCGAAAATGCCGTGGCCAGAAGTGATTGCATTATTAATTCATTATTCAAAATTACAAATGGCTGGTGATAAAGGCCA
GTATTATGCAAACAGAGTAAAACAATGGCTAGTTTATCTTCGACAAGAATATACTGAAGCTAAAGATATTTTCATGGATA
TTCGAAGCCATAAAAAAGCGCCTTTAATTGTAGAGCGCTTAAATCAAGAATTAGAGAACGTTAGTTAA

Upstream 100 bases:

>100_bases
CTCTACAGGGGAGACGATAACTAAAAGCTATAATAAAGGAACTAAGTGACATCTAGCCCCTTTCCCCCTACAATCCCACC
TCCAATAAAAGAGGTGGGAT

Downstream 100 bases:

>100_bases
TCGCTATTACTCATCGTGTTTATGCAAAGTAAACACGATTCTTTCCATCTTCTTTGGCTTTATACAACAAATCATCGGCT
TTTTTAATAATGTGTTCAAA

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRIVDQLLPPHVFHRICPELHNDSKTKSGTPIRLQLLGQEPNWMAEN
AARAIDLGSDGLDLNFGCPAKAVNKSRGGAALLKDPELIYQIVKACREAVPQDKKVTAKIRLGWENPEECFAIADAIKQA
GANELTIHARTKTDGYRAEEIKWDYINQVRQKIDIPIIANGEIWNYADGQRCIAATGIDSLMVCRGAFNMPNLGNVVKHN
HPKMPWPEVIALLIHYSKLQMAGDKGQYYANRVKQWLVYLRQEYTEAKDIFMDIRSHKKAPLIVERLNQELENVS

Sequences:

>Translated_315_residues
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRIVDQLLPPHVFHRICPELHNDSKTKSGTPIRLQLLGQEPNWMAEN
AARAIDLGSDGLDLNFGCPAKAVNKSRGGAALLKDPELIYQIVKACREAVPQDKKVTAKIRLGWENPEECFAIADAIKQA
GANELTIHARTKTDGYRAEEIKWDYINQVRQKIDIPIIANGEIWNYADGQRCIAATGIDSLMVCRGAFNMPNLGNVVKHN
HPKMPWPEVIALLIHYSKLQMAGDKGQYYANRVKQWLVYLRQEYTEAKDIFMDIRSHKKAPLIVERLNQELENVS
>Mature_315_residues
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRIVDQLLPPHVFHRICPELHNDSKTKSGTPIRLQLLGQEPNWMAEN
AARAIDLGSDGLDLNFGCPAKAVNKSRGGAALLKDPELIYQIVKACREAVPQDKKVTAKIRLGWENPEECFAIADAIKQA
GANELTIHARTKTDGYRAEEIKWDYINQVRQKIDIPIIANGEIWNYADGQRCIAATGIDSLMVCRGAFNMPNLGNVVKHN
HPKMPWPEVIALLIHYSKLQMAGDKGQYYANRVKQWLVYLRQEYTEAKDIFMDIRSHKKAPLIVERLNQELENVS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=193, Percent_Identity=29.5336787564767, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI31742496, Length=178, Percent_Identity=26.9662921348315, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI239788483, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=27.3809523809524, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788462, Length=303, Percent_Identity=61.7161716171617, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789660, Length=261, Percent_Identity=33.7164750957854, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=186, Percent_Identity=32.258064516129, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=156, Percent_Identity=31.4102564102564, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17510279, Length=224, Percent_Identity=29.9107142857143, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=27.363184079602, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323437, Length=147, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921524, Length=174, Percent_Identity=32.183908045977, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24580595, Length=173, Percent_Identity=34.1040462427746, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920448, Length=173, Percent_Identity=34.1040462427746, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=184, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=31.9502074688797, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35728; Mature: 35728

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRIVDQLLPPHVFHRICPELHNDSKTK
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
SGTPIRLQLLGQEPNWMAENAARAIDLGSDGLDLNFGCPAKAVNKSRGGAALLKDPELIY
CCCCEEEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCCHHHHCCCCCCEEECCCHHHHH
QIVKACREAVPQDKKVTAKIRLGWENPEECFAIADAIKQAGANELTIHARTKTDGYRAEE
HHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
IKWDYINQVRQKIDIPIIANGEIWNYADGQRCIAATGIDSLMVCRGAFNMPNLGNVVKHN
CCHHHHHHHHHHCCCCEEECCCEECCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHCC
HPKMPWPEVIALLIHYSKLQMAGDKGQYYANRVKQWLVYLRQEYTEAKDIFMDIRSHKKA
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLIVERLNQELENVS
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVILGPMEGVLDHLMRELLTEINDYDFCVTEFVRIVDQLLPPHVFHRICPELHNDSKTK
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
SGTPIRLQLLGQEPNWMAENAARAIDLGSDGLDLNFGCPAKAVNKSRGGAALLKDPELIY
CCCCEEEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCCHHHHCCCCCCEEECCCHHHHH
QIVKACREAVPQDKKVTAKIRLGWENPEECFAIADAIKQAGANELTIHARTKTDGYRAEE
HHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHH
IKWDYINQVRQKIDIPIIANGEIWNYADGQRCIAATGIDSLMVCRGAFNMPNLGNVVKHN
CCHHHHHHHHHHCCCCEEECCCEECCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHCC
HPKMPWPEVIALLIHYSKLQMAGDKGQYYANRVKQWLVYLRQEYTEAKDIFMDIRSHKKA
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLIVERLNQELENVS
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]