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The map label for this gene is pldA [H]

Identifier: 209695452

GI number: 209695452

Start: 2136897

End: 2137670

Strand: Direct

Name: pldA [H]

Synonym: VSAL_I1995

Alternate gene names: 209695452

Gene position: 2136897-2137670 (Clockwise)

Preceding gene: 209695451

Following gene: 209695453

Centisome position: 64.26

GC content: 38.5

Gene sequence:

>774_bases
ATGAAAAAATGGATGCTATTAATTGCATGGGCTCTACCCTTTATGACATCTGCAAAAGCGCTTAATCGTATATCTGGTTA
TGAAGACAGCTATTTATTAGGAAGTTACACCGATAGTATCAACAAAGATGAATACATTTCAGGAGGCTTTGAAGACGCTG
ATGGCCTTCAAAATGTGGAAGTGAAATTTCAGTTTTCTTTAGCGGTGCCGATCGTTCAAATTAATAACAGCACTTCATTC
ATGTTCTCTTATACGCAAAAGTCGCTATGGCAACTAGGGAATAGCGAGATTTCATCGCCTTTTAGAGAAACGAACTATAA
GCCTCAGTTTTTTGTTATGCATCAATCAAACATGCTGATTTTTAATAGTGCAGAATTTGGTTATATGCATGAATCAAATG
GTCAAACATCGAGTTTATCACGCAGTTGGGATCGTATTTATACTGGATTAGAGCGTCTTGATGGCCCAGTGCAATACGGT
GTTCGTGCATGGTATGTGTTCAGCGACGAAGAAAACAATGAAGACATTAATGATTTTATGGCTCCTTATGATGTGTGGCT
AAAAGTCGGTAATGATATTGGTGAATTTTCTACACGATTCCATTACAACTTTGCTACCGATAAAGGTGGCGTAGAGGCGG
CGTATACACTTTATGTAAACCAGTTTGTCGGTCTCTATGTACAAGGTTGGACGGGGTATGGTGAGACGCTGATTGATTAT
AATTATGATCACAGTCGCATTGGTATTGGTATGAGATTACTTCCACCAAATTAA

Upstream 100 bases:

>100_bases
CATTGATATTAAATAATTAATAATGGAACGCTTGTGTCATTATAGGTATGATTTTTGCTTTACTAGTGATTAAAGGCAAG
TTACTGACAAAAGGTTAATT

Downstream 100 bases:

>100_bases
TTGACCGTTTAATCACACGATAAAAAAGCTTCAGCAGGTCTGAGGCTTTTTTTGTGATTAAGGTAATGATGGTGATCATT
GCTATCACTCTTCATTTACT

Product: phospholipase A1 precursor

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; OMPLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKWMLLIAWALPFMTSAKALNRISGYEDSYLLGSYTDSINKDEYISGGFEDADGLQNVEVKFQFSLAVPIVQINNSTSF
MFSYTQKSLWQLGNSEISSPFRETNYKPQFFVMHQSNMLIFNSAEFGYMHESNGQTSSLSRSWDRIYTGLERLDGPVQYG
VRAWYVFSDEENNEDINDFMAPYDVWLKVGNDIGEFSTRFHYNFATDKGGVEAAYTLYVNQFVGLYVQGWTGYGETLIDY
NYDHSRIGIGMRLLPPN

Sequences:

>Translated_257_residues
MKKWMLLIAWALPFMTSAKALNRISGYEDSYLLGSYTDSINKDEYISGGFEDADGLQNVEVKFQFSLAVPIVQINNSTSF
MFSYTQKSLWQLGNSEISSPFRETNYKPQFFVMHQSNMLIFNSAEFGYMHESNGQTSSLSRSWDRIYTGLERLDGPVQYG
VRAWYVFSDEENNEDINDFMAPYDVWLKVGNDIGEFSTRFHYNFATDKGGVEAAYTLYVNQFVGLYVQGWTGYGETLIDY
NYDHSRIGIGMRLLPPN
>Mature_257_residues
MKKWMLLIAWALPFMTSAKALNRISGYEDSYLLGSYTDSINKDEYISGGFEDADGLQNVEVKFQFSLAVPIVQINNSTSF
MFSYTQKSLWQLGNSEISSPFRETNYKPQFFVMHQSNMLIFNSAEFGYMHESNGQTSSLSRSWDRIYTGLERLDGPVQYG
VRAWYVFSDEENNEDINDFMAPYDVWLKVGNDIGEFSTRFHYNFATDKGGVEAAYTLYVNQFVGLYVQGWTGYGETLIDY
NYDHSRIGIGMRLLPPN

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=235, Percent_Identity=40, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 29542; Mature: 29542

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWMLLIAWALPFMTSAKALNRISGYEDSYLLGSYTDSINKDEYISGGFEDADGLQNVE
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
VKFQFSLAVPIVQINNSTSFMFSYTQKSLWQLGNSEISSPFRETNYKPQFFVMHQSNMLI
EEEEEEEEEEEEEECCCCEEEEEHHHHHHHHCCCHHHCCCHHHCCCCCEEEEEECCCEEE
FNSAEFGYMHESNGQTSSLSRSWDRIYTGLERLDGPVQYGVRAWYVFSDEENNEDINDFM
EECCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEECEEEEEEECCCCCCCCHHHHH
APYDVWLKVGNDIGEFSTRFHYNFATDKGGVEAAYTLYVNQFVGLYVQGWTGYGETLIDY
CHHHHHEEECCCHHCCCEEEEEEEECCCCCCCEEEEEEEHHEEEEEEEECCCCCCEEEEC
NYDHSRIGIGMRLLPPN
CCCCCEEEEEEEEECCC
>Mature Secondary Structure
MKKWMLLIAWALPFMTSAKALNRISGYEDSYLLGSYTDSINKDEYISGGFEDADGLQNVE
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEE
VKFQFSLAVPIVQINNSTSFMFSYTQKSLWQLGNSEISSPFRETNYKPQFFVMHQSNMLI
EEEEEEEEEEEEEECCCCEEEEEHHHHHHHHCCCHHHCCCHHHCCCCCEEEEEECCCEEE
FNSAEFGYMHESNGQTSSLSRSWDRIYTGLERLDGPVQYGVRAWYVFSDEENNEDINDFM
EECCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEECEEEEEEECCCCCCCCHHHHH
APYDVWLKVGNDIGEFSTRFHYNFATDKGGVEAAYTLYVNQFVGLYVQGWTGYGETLIDY
CHHHHHEEECCCHHCCCEEEEEEEECCCCCCCEEEEEEEHHEEEEEEEECCCCCCEEEEC
NYDHSRIGIGMRLLPPN
CCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]