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The map label for this gene is folE

Identifier: 209695396

GI number: 209695396

Start: 2067717

End: 2068370

Strand: Direct

Name: folE

Synonym: VSAL_I1933

Alternate gene names: 209695396

Gene position: 2067717-2068370 (Clockwise)

Preceding gene: 209695393

Following gene: 209695398

Centisome position: 62.18

GC content: 40.98

Gene sequence:

>654_bases
ATGTCATCATTCAGTGAATCAGCCATTTTGGTGCGTGATGCTCTTGTTGCGCGTGGCCTTGAGACGCCAATGAAAGAAAA
TGGAATTAGTCGTGAAGAGAAAAAAGAACGTATTGAAGAGCATATGCGTGAGATTTTATCTCTGCTTACTCTCGACCTTA
GTGATGACAGCCTTGAAGAGACGCCTCATCGAATTGCTAAGATGTACGTTGATGAAATCTTTTCTGGGTTAGATTATGCG
AATTTCCCTAAAATTACCGTCATTGAAAATAAAATGAACTGTGATGAAATGGTTCGAGTGAACGACATTACATTAACGAG
TACGTGTGAACATCATTTAGTGACTATTGATGGAAAAGCCACCGTTGCTTATATTCCTCGTGAAAAGATCATTGGTCTGT
CGAAGATTAATCGTATTGTTCGATTTTTTGCCCAGCGTCCTCAAGTTCAAGAGCGTATGACACAGCAAATATTAGTGGCA
CTGCAAACATTACTTGGCAGTGATGATGTTGCGATCACAATGGAAGCGACACATTATTGTGTTAAGTCTCGTGGTGTCAT
GGATGCGACGAGTAGCACAACGACAACTGCGCTTGGCGGGATTTTCAAACGTAATCCAGCGACAAGACATGAATTTTTAA
GTGGCATTCGTTAA

Upstream 100 bases:

>100_bases
GCTGAACATCTAATAGACCCATGAAAATCAGGGTGTATTTATTTTACAGAGCGGGTATCCTTAGCGCAGGATTTTTAAAT
AGAATACTCTGGAGTATATG

Downstream 100 bases:

>100_bases
TATTTACGGATGTTAAAAAAGCCCACGATAATTAAATTATCGTGGGCTTTTTAATTTATGCTTTGTTTTAAAGTAAGGGG
CTAAACATAGAGAAGAAACG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSSFSESAILVRDALVARGLETPMKENGISREEKKERIEEHMREILSLLTLDLSDDSLEETPHRIAKMYVDEIFSGLDYA
NFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKATVAYIPREKIIGLSKINRIVRFFAQRPQVQERMTQQILVA
LQTLLGSDDVAITMEATHYCVKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR

Sequences:

>Translated_217_residues
MSSFSESAILVRDALVARGLETPMKENGISREEKKERIEEHMREILSLLTLDLSDDSLEETPHRIAKMYVDEIFSGLDYA
NFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKATVAYIPREKIIGLSKINRIVRFFAQRPQVQERMTQQILVA
LQTLLGSDDVAITMEATHYCVKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR
>Mature_216_residues
SSFSESAILVRDALVARGLETPMKENGISREEKKERIEEHMREILSLLTLDLSDDSLEETPHRIAKMYVDEIFSGLDYAN
FPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKATVAYIPREKIIGLSKINRIVRFFAQRPQVQERMTQQILVAL
QTLLGSDDVAITMEATHYCVKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=41.1111111111111, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=41.1111111111111, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI66932970, Length=155, Percent_Identity=38.0645161290323, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=40, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788476, Length=219, Percent_Identity=71.2328767123288, Blast_Score=314, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17560486, Length=200, Percent_Identity=39, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=42.5414364640884, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24656796, Length=120, Percent_Identity=48.3333333333333, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656782, Length=120, Percent_Identity=48.3333333333333, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656791, Length=120, Percent_Identity=48.3333333333333, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_ALISL (B6EGN8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263325.1
- GeneID:   6988819
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1933
- HOGENOM:   HBG370191
- OMA:   ARFMSMI
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24475; Mature: 24344

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFSESAILVRDALVARGLETPMKENGISREEKKERIEEHMREILSLLTLDLSDDSLEE
CCCCCHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TPHRIAKMYVDEIFSGLDYANFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHEEECCEEEECCCCCEEEEECCCE
TVAYIPREKIIGLSKINRIVRFFAQRPQVQERMTQQILVALQTLLGSDDVAITMEATHYC
EEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
VKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SSFSESAILVRDALVARGLETPMKENGISREEKKERIEEHMREILSLLTLDLSDDSLEE
CCCCHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TPHRIAKMYVDEIFSGLDYANFPKITVIENKMNCDEMVRVNDITLTSTCEHHLVTIDGKA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHEEECCEEEECCCCCEEEEECCCE
TVAYIPREKIIGLSKINRIVRFFAQRPQVQERMTQQILVALQTLLGSDDVAITMEATHYC
EEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
VKSRGVMDATSSTTTTALGGIFKRNPATRHEFLSGIR
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA