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The map label for this gene is luxO [H]

Identifier: 209695341

GI number: 209695341

Start: 2010023

End: 2011432

Strand: Direct

Name: luxO [H]

Synonym: VSAL_I1874

Alternate gene names: 209695341

Gene position: 2010023-2011432 (Clockwise)

Preceding gene: 209695340

Following gene: 209695342

Centisome position: 60.45

GC content: 39.93

Gene sequence:

>1410_bases
ATGGTTGAAGACACCGCTTCTGTTGCTGCGCTTTATCGTTCTTACCTTAATCCTTTAGGTTTAGATATCGATGTTGTAGC
AAAAGGTGTTGATGCGATTGAAAAAGTGACGTTAAAGGCACCGGATTTAATTTTATTGGATTTGCGCTTGCCGGATATGA
CTGGACTTGAGGTTTTAACGGAAATTCGAAAAATTCATCATGATACACCTGTTGTGTTAATGACTGCCCATGGTTCTATT
GATGTTGCCGTTGAAGCGATGCGTTTAGGTGCTCAGGATTTTCTTATTAAACCATGTGAAGCTGATAGACTTAGAGTCAC
TGTAAATAGTGCATTAAGAAGAGCGCAAAAGAACTTAGATAATACGGATCTTGATCCCAATAAAAGCAACAATCAATACC
AAGGTTTTATTGGTAGTAGCTCTCAAATGCATCAAGTATATCGAACTATTGATTCTGCGGCGCCAAGTAAAGCCACGGTC
TTTATTACTGGGGAATCAGGAACAGGTAAAGAAGTGTGTGCCGAAGCAGTTCACGCAGCAAGTAAGCGGGGAGACGGTCC
ATTTATCGCGATTAACTGTGCCGCGATACCGAAAGACTTGATTGAAAGTGAGTTGTTTGGTCATGTTAAAGGCGCATTTA
CAGGAGCGTCAGTTGATAGGAAAGGGGCGGCAGAACAAGCTGACGGAGGAACCCTGTTCTTAGATGAATTGTGCGAAATG
GATTTAGATCTGCAAACCAAATTATTACGTTTTATCCAAACAGGGACCTTTAAAAAAGTAGGTTCTTCTAAAATGAGTCG
AGTTGATGTTCGATTTGTTTGTGCAACCAACCGCAATCCTTGGCTAGAAGTTCAAGCAGGTCGATTCCGTGAAGATTTAT
ATTATCGATTGCATGTTATCCCATTAACGCTGCCTCCGTTACGGGAAAGAGGCAATGATATTATTGAAATTGGTCACTCA
ATATTAGGTCACTTTTCTCATGAAGAAGGGAGAGAATTTATTACATTTAGCCCAGATGTGGTTTCTCGATTTCTTGAATA
TGATTGGCCGGGAAACGTTCGGCAATTACAAAACGTCATTAGAAATGTCGTGGTATTGAATCGGGGTAAAGAAGTCTTGC
TTTCTATGCTTCCGCCACCGTTAGCGCCAGAAGATAATAATGGTTCGAATATTGCATCTTTACAAGCAGTTAATCGATTA
AGCCGTCATTTTGAACAACTTCCAGTTGATATTGAATTCGTACCTGAAGGCAATATGAAAAAAGAAATTAGACCATTATG
GTTGTCTGAAAAGCAGATTATAGAAGATGCAATTACTCTATGTGATGGGAACATTCCACGAGCCGCTAAACAATTAGAGG
TGAGCCCTTCCACAATATATAGAAAATTACAGTCATGGAATGATAAATAG

Upstream 100 bases:

>100_bases
GCGAAAATATCTTTGCGTTATGCAATTGCATTATGCGAAAATGCAAAGGTTGATATGCTTTTTAATGGTTTGGATTCTGA
TGCAAAAAAAATATTTACTC

Downstream 100 bases:

>100_bases
AGGGTGATACCGTGAATCAATATGTAAATCAACTAGCCGTCAATCAGTTAGTGCAGGAAGTTGGTGATGAGAATGTGCCC
TTTTTATTTGGTATTTTCTG

Product: two component signal response regulator LuxO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MVEDTASVAALYRSYLNPLGLDIDVVAKGVDAIEKVTLKAPDLILLDLRLPDMTGLEVLTEIRKIHHDTPVVLMTAHGSI
DVAVEAMRLGAQDFLIKPCEADRLRVTVNSALRRAQKNLDNTDLDPNKSNNQYQGFIGSSSQMHQVYRTIDSAAPSKATV
FITGESGTGKEVCAEAVHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTLFLDELCEM
DLDLQTKLLRFIQTGTFKKVGSSKMSRVDVRFVCATNRNPWLEVQAGRFREDLYYRLHVIPLTLPPLRERGNDIIEIGHS
ILGHFSHEEGREFITFSPDVVSRFLEYDWPGNVRQLQNVIRNVVVLNRGKEVLLSMLPPPLAPEDNNGSNIASLQAVNRL
SRHFEQLPVDIEFVPEGNMKKEIRPLWLSEKQIIEDAITLCDGNIPRAAKQLEVSPSTIYRKLQSWNDK

Sequences:

>Translated_469_residues
MVEDTASVAALYRSYLNPLGLDIDVVAKGVDAIEKVTLKAPDLILLDLRLPDMTGLEVLTEIRKIHHDTPVVLMTAHGSI
DVAVEAMRLGAQDFLIKPCEADRLRVTVNSALRRAQKNLDNTDLDPNKSNNQYQGFIGSSSQMHQVYRTIDSAAPSKATV
FITGESGTGKEVCAEAVHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTLFLDELCEM
DLDLQTKLLRFIQTGTFKKVGSSKMSRVDVRFVCATNRNPWLEVQAGRFREDLYYRLHVIPLTLPPLRERGNDIIEIGHS
ILGHFSHEEGREFITFSPDVVSRFLEYDWPGNVRQLQNVIRNVVVLNRGKEVLLSMLPPPLAPEDNNGSNIASLQAVNRL
SRHFEQLPVDIEFVPEGNMKKEIRPLWLSEKQIIEDAITLCDGNIPRAAKQLEVSPSTIYRKLQSWNDK
>Mature_469_residues
MVEDTASVAALYRSYLNPLGLDIDVVAKGVDAIEKVTLKAPDLILLDLRLPDMTGLEVLTEIRKIHHDTPVVLMTAHGSI
DVAVEAMRLGAQDFLIKPCEADRLRVTVNSALRRAQKNLDNTDLDPNKSNNQYQGFIGSSSQMHQVYRTIDSAAPSKATV
FITGESGTGKEVCAEAVHAASKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTLFLDELCEM
DLDLQTKLLRFIQTGTFKKVGSSKMSRVDVRFVCATNRNPWLEVQAGRFREDLYYRLHVIPLTLPPLRERGNDIIEIGHS
ILGHFSHEEGREFITFSPDVVSRFLEYDWPGNVRQLQNVIRNVVVLNRGKEVLLSMLPPPLAPEDNNGSNIASLQAVNRL
SRHFEQLPVDIEFVPEGNMKKEIRPLWLSEKQIIEDAITLCDGNIPRAAKQLEVSPSTIYRKLQSWNDK

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=39.3162393162393, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=37.7969762419007, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=39.6551724137931, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788905, Length=349, Percent_Identity=39.8280802292264, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=41.6666666666667, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082152, Length=262, Percent_Identity=46.9465648854962, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87082117, Length=316, Percent_Identity=40.8227848101266, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789087, Length=297, Percent_Identity=41.4141414141414, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787583, Length=240, Percent_Identity=42.9166666666667, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1786524, Length=349, Percent_Identity=38.10888252149, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081872, Length=234, Percent_Identity=43.5897435897436, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789828, Length=237, Percent_Identity=36.2869198312236, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=27.8846153846154, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787487, Length=113, Percent_Identity=36.283185840708, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=31.304347826087, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789402, Length=134, Percent_Identity=29.1044776119403, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=28.2051282051282, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52086; Mature: 52086

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDTASVAALYRSYLNPLGLDIDVVAKGVDAIEKVTLKAPDLILLDLRLPDMTGLEVLT
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
EIRKIHHDTPVVLMTAHGSIDVAVEAMRLGAQDFLIKPCEADRLRVTVNSALRRAQKNLD
HHHHHHCCCCEEEEEECCCHHHHHHHHHCCCHHHEECCCCCCCEEEEHHHHHHHHHHCCC
NTDLDPNKSNNQYQGFIGSSSQMHQVYRTIDSAAPSKATVFITGESGTGKEVCAEAVHAA
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHH
SKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTLFLDELCEM
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHCC
DLDLQTKLLRFIQTGTFKKVGSSKMSRVDVRFVCATNRNPWLEVQAGRFREDLYYRLHVI
CCCHHHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCCEEEEECCHHHHCEEEEEEEE
PLTLPPLRERGNDIIEIGHSILGHFSHEEGREFITFSPDVVSRFLEYDWPGNVRQLQNVI
EECCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHHHH
RNVVVLNRGKEVLLSMLPPPLAPEDNNGSNIASLQAVNRLSRHFEQLPVDIEFVPEGNMK
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
KEIRPLWLSEKQIIEDAITLCDGNIPRAAKQLEVSPSTIYRKLQSWNDK
HHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVEDTASVAALYRSYLNPLGLDIDVVAKGVDAIEKVTLKAPDLILLDLRLPDMTGLEVLT
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH
EIRKIHHDTPVVLMTAHGSIDVAVEAMRLGAQDFLIKPCEADRLRVTVNSALRRAQKNLD
HHHHHHCCCCEEEEEECCCHHHHHHHHHCCCHHHEECCCCCCCEEEEHHHHHHHHHHCCC
NTDLDPNKSNNQYQGFIGSSSQMHQVYRTIDSAAPSKATVFITGESGTGKEVCAEAVHAA
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHH
SKRGDGPFIAINCAAIPKDLIESELFGHVKGAFTGASVDRKGAAEQADGGTLFLDELCEM
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHCC
DLDLQTKLLRFIQTGTFKKVGSSKMSRVDVRFVCATNRNPWLEVQAGRFREDLYYRLHVI
CCCHHHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCCEEEEECCHHHHCEEEEEEEE
PLTLPPLRERGNDIIEIGHSILGHFSHEEGREFITFSPDVVSRFLEYDWPGNVRQLQNVI
EECCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHHHHHH
RNVVVLNRGKEVLLSMLPPPLAPEDNNGSNIASLQAVNRLSRHFEQLPVDIEFVPEGNMK
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
KEIRPLWLSEKQIIEDAITLCDGNIPRAAKQLEVSPSTIYRKLQSWNDK
HHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]