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The map label for this gene is mscS [H]

Identifier: 209695272

GI number: 209695272

Start: 1919516

End: 1921201

Strand: Direct

Name: mscS [H]

Synonym: VSAL_I1797

Alternate gene names: 209695272

Gene position: 1919516-1921201 (Clockwise)

Preceding gene: 209695271

Following gene: 209695274

Centisome position: 57.73

GC content: 39.09

Gene sequence:

>1686_bases
ATGCGCTATTTACTTTGTTTTCTTCTTTTATTTTCATCCCTTACCTATGCCAATGAAACTCAAGACATCGAGCAATTACA
AAATATTAATGCTGAAATTGCACGACTAAAGCAAGAGTCTGCCTCATTCAAAGGCAATGACTTAGCGGTTATTCGCGTAC
AGCAAGTAGAAAAAAACAATGAACTCAGAATCGTTCTGTCTGAGTTAATCAACAAGCACACCGCAGAAAATACGCCACTG
TTGGTGAGTGAAGTTGAAGATCAAATTCGTTATGCTGATCGTTCTTTTCTCTATTTAGGCAAAGAGCTAAAATCAAAAAA
AGATAAAATAGATACGGCGGATGTCGAGCATAAACTCGCTTTACAAAACACATTAAATGAATCAAGAAGTTATTATTACA
CTTCCCTCTCAGATCAACTTCAAAATTACCGCTGGTTAAACAAACTCGGCAAACCAAACCAAGCAAAAGTCGATGCATTC
AAGTTAGTTATCGACCAAAAAATTAAGTTTCTGTCTGCCTCTTTGAGTTTTGATTCAAGCAGAGAACAGCTATTAGCGGA
TCAACTTCAGCGTTCGTCTGATTCAGAGAAATCCTCTCTGACCTTAGAGCACCTCTTCTTTCAAAGAAAAGTGACCAACT
CAACGGATGGGTTAATTAAACTGATTAACATTGCTGACCAATTAAATATCAGCACGACAAACTACAAACGTCAGGTCTTT
GCCACTACGGGCAATTTAACAGAAGATATTTTAAGTTTTCCCGTGATGGCAGCGATTTTAAGTAATTGGATCACCGACGG
CAGTAACTGGTTATTCAAACACGCACCTGATCAGCTCTTAAAATTCTTTGTCTTTATCTTAATCATTTTTGCCACTCGTT
TATTAGCAAGGCTGACTCGACGTGTGGTACTCAAAGCCGTTGTTCAACCTCATTTACGTATGAGTAAATTGATGCAAGAT
TTCTTTGTTTCGATGTCGAGTAAACTGATTTTCTTCATTGGTATTCTCATTGCCTTTTCTAAAATTGGGTTGGATTTGGC
TCCGGTTCTCACCGGTTTCGGTGTTGCTGGTATTATTATTGGTTTTGCATTGCAAGATACGCTATCCAACTTTGCATCAG
GCATGATGCTGCTAATTTATCGCCCGTTTGATGTGGGTGATTTAGTGGATGCAGGGGGCGTATCAGGAAAAGTGAGCCAC
ATGAGTTTAGTAAACACCACGATTAAAACGTTTGATAACCAAATTATCATCTTACCCAACAGTAAAATTTGGGGAGACGT
AATAAAAAACGTAACTCACGAACGATTACGCCGTGTCGATATGGTGTTTGGCATTGGCTACGAAGACAGCATCGAACACG
CTGAAAAAATCTTAGCCGAAATTATTGATACTCACCCTGCGGCCTTAAAAAAACCAGAGCCCAATATTCGAGTACATACT
CTTGGTGCCTCATCGGTTGATTTTATTGTACGACCTTGGGTAAAAACCGAAGATTATTGGGATGTGTATTGGGATGTGAC
TCGTGAAGTGAAATTACGCTTTGATAAAGAAGGGATCTCGATTCCTTTTGCTCAACAAGATGTGCATGTACATTTTGCTA
AAAAAGCGAAGAAACCAAAAGCAACAACAGGGATAGCGGGAAAACTGAGTAACGTCATTGACTCTGTTCGATCAAAAGAC
AGCTAA

Upstream 100 bases:

>100_bases
TGACAAAGCTTTATCAAAAATACCTGCCACTCCCGATGCATTTTATCTACAATAGTACTTTAAGTTACATTGACCCACCC
TTTACGGTCATAGAGGTAAT

Downstream 100 bases:

>100_bases
ACCATCCCATAAAAAAGAGCATCTTAAATTAGGTGCTCTTTTCAAGAATAGCATTCTTATTTTATCGACATCATCAATTT
ATTTTGAAACCAACAAATTG

Product: mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRYLLCFLLLFSSLTYANETQDIEQLQNINAEIARLKQESASFKGNDLAVIRVQQVEKNNELRIVLSELINKHTAENTPL
LVSEVEDQIRYADRSFLYLGKELKSKKDKIDTADVEHKLALQNTLNESRSYYYTSLSDQLQNYRWLNKLGKPNQAKVDAF
KLVIDQKIKFLSASLSFDSSREQLLADQLQRSSDSEKSSLTLEHLFFQRKVTNSTDGLIKLINIADQLNISTTNYKRQVF
ATTGNLTEDILSFPVMAAILSNWITDGSNWLFKHAPDQLLKFFVFILIIFATRLLARLTRRVVLKAVVQPHLRMSKLMQD
FFVSMSSKLIFFIGILIAFSKIGLDLAPVLTGFGVAGIIIGFALQDTLSNFASGMMLLIYRPFDVGDLVDAGGVSGKVSH
MSLVNTTIKTFDNQIIILPNSKIWGDVIKNVTHERLRRVDMVFGIGYEDSIEHAEKILAEIIDTHPAALKKPEPNIRVHT
LGASSVDFIVRPWVKTEDYWDVYWDVTREVKLRFDKEGISIPFAQQDVHVHFAKKAKKPKATTGIAGKLSNVIDSVRSKD
S

Sequences:

>Translated_561_residues
MRYLLCFLLLFSSLTYANETQDIEQLQNINAEIARLKQESASFKGNDLAVIRVQQVEKNNELRIVLSELINKHTAENTPL
LVSEVEDQIRYADRSFLYLGKELKSKKDKIDTADVEHKLALQNTLNESRSYYYTSLSDQLQNYRWLNKLGKPNQAKVDAF
KLVIDQKIKFLSASLSFDSSREQLLADQLQRSSDSEKSSLTLEHLFFQRKVTNSTDGLIKLINIADQLNISTTNYKRQVF
ATTGNLTEDILSFPVMAAILSNWITDGSNWLFKHAPDQLLKFFVFILIIFATRLLARLTRRVVLKAVVQPHLRMSKLMQD
FFVSMSSKLIFFIGILIAFSKIGLDLAPVLTGFGVAGIIIGFALQDTLSNFASGMMLLIYRPFDVGDLVDAGGVSGKVSH
MSLVNTTIKTFDNQIIILPNSKIWGDVIKNVTHERLRRVDMVFGIGYEDSIEHAEKILAEIIDTHPAALKKPEPNIRVHT
LGASSVDFIVRPWVKTEDYWDVYWDVTREVKLRFDKEGISIPFAQQDVHVHFAKKAKKPKATTGIAGKLSNVIDSVRSKD
S
>Mature_561_residues
MRYLLCFLLLFSSLTYANETQDIEQLQNINAEIARLKQESASFKGNDLAVIRVQQVEKNNELRIVLSELINKHTAENTPL
LVSEVEDQIRYADRSFLYLGKELKSKKDKIDTADVEHKLALQNTLNESRSYYYTSLSDQLQNYRWLNKLGKPNQAKVDAF
KLVIDQKIKFLSASLSFDSSREQLLADQLQRSSDSEKSSLTLEHLFFQRKVTNSTDGLIKLINIADQLNISTTNYKRQVF
ATTGNLTEDILSFPVMAAILSNWITDGSNWLFKHAPDQLLKFFVFILIIFATRLLARLTRRVVLKAVVQPHLRMSKLMQD
FFVSMSSKLIFFIGILIAFSKIGLDLAPVLTGFGVAGIIIGFALQDTLSNFASGMMLLIYRPFDVGDLVDAGGVSGKVSH
MSLVNTTIKTFDNQIIILPNSKIWGDVIKNVTHERLRRVDMVFGIGYEDSIEHAEKILAEIIDTHPAALKKPEPNIRVHT
LGASSVDFIVRPWVKTEDYWDVYWDVTREVKLRFDKEGISIPFAQQDVHVHFAKKAKKPKATTGIAGKLSNVIDSVRSKD
S

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=281, Percent_Identity=34.5195729537367, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI1786670, Length=234, Percent_Identity=26.4957264957265, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787591, Length=204, Percent_Identity=27.9411764705882, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367355, Length=145, Percent_Identity=29.6551724137931, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 63557; Mature: 63557

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLLCFLLLFSSLTYANETQDIEQLQNINAEIARLKQESASFKGNDLAVIRVQQVEKNN
CHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
ELRIVLSELINKHTAENTPLLVSEVEDQIRYADRSFLYLGKELKSKKDKIDTADVEHKLA
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHH
LQNTLNESRSYYYTSLSDQLQNYRWLNKLGKPNQAKVDAFKLVIDQKIKFLSASLSFDSS
HHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCH
REQLLADQLQRSSDSEKSSLTLEHLFFQRKVTNSTDGLIKLINIADQLNISTTNYKRQVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
ATTGNLTEDILSFPVMAAILSNWITDGSNWLFKHAPDQLLKFFVFILIIFATRLLARLTR
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLKAVVQPHLRMSKLMQDFFVSMSSKLIFFIGILIAFSKIGLDLAPVLTGFGVAGIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHH
GFALQDTLSNFASGMMLLIYRPFDVGDLVDAGGVSGKVSHMSLVNTTIKTFDNQIIILPN
HHHHHHHHHHHHCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
SKIWGDVIKNVTHERLRRVDMVFGIGYEDSIEHAEKILAEIIDTHPAALKKPEPNIRVHT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEE
LGASSVDFIVRPWVKTEDYWDVYWDVTREVKLRFDKEGISIPFAQQDVHVHFAKKAKKPK
ECCCCCCHHEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCCC
ATTGIAGKLSNVIDSVRSKDS
CCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRYLLCFLLLFSSLTYANETQDIEQLQNINAEIARLKQESASFKGNDLAVIRVQQVEKNN
CHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
ELRIVLSELINKHTAENTPLLVSEVEDQIRYADRSFLYLGKELKSKKDKIDTADVEHKLA
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHH
LQNTLNESRSYYYTSLSDQLQNYRWLNKLGKPNQAKVDAFKLVIDQKIKFLSASLSFDSS
HHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCH
REQLLADQLQRSSDSEKSSLTLEHLFFQRKVTNSTDGLIKLINIADQLNISTTNYKRQVF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
ATTGNLTEDILSFPVMAAILSNWITDGSNWLFKHAPDQLLKFFVFILIIFATRLLARLTR
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLKAVVQPHLRMSKLMQDFFVSMSSKLIFFIGILIAFSKIGLDLAPVLTGFGVAGIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHH
GFALQDTLSNFASGMMLLIYRPFDVGDLVDAGGVSGKVSHMSLVNTTIKTFDNQIIILPN
HHHHHHHHHHHHCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
SKIWGDVIKNVTHERLRRVDMVFGIGYEDSIEHAEKILAEIIDTHPAALKKPEPNIRVHT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEE
LGASSVDFIVRPWVKTEDYWDVYWDVTREVKLRFDKEGISIPFAQQDVHVHFAKKAKKPK
ECCCCCCHHEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCCC
ATTGIAGKLSNVIDSVRSKDS
CCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]