Click here to switch to the map view.

The map label for this gene is rsuA [H]

Identifier: 209694915

GI number: 209694915

Start: 1505861

End: 1506550

Strand: Direct

Name: rsuA [H]

Synonym: VSAL_I1389

Alternate gene names: 209694915

Gene position: 1505861-1506550 (Clockwise)

Preceding gene: 209694914

Following gene: 209694917

Centisome position: 45.29

GC content: 37.25

Gene sequence:

>690_bases
ATGCGCCTTGATAAATTTGTTTGTAAAAGTACGACGTTAACAAAAAACGAAGCGCTTGAATGCATTGCTGAGGGAAGAGT
AAAAGTGAATGCTAAGATTGTTACCGTTGAATCGACTCAAGTGCATGAGAACAACCATGTGACACTCAATGGTGATTTAT
TGACTGCTCGTCCTTTTCGCTACTTACTCATGAATAAACCTGCTAATACGATTTGTTCAAACATCGATGAAGCCTATCCC
TCCTTATTCAACTATATTAAAGAAGGTAATAATTCGGAATTGCACATTGCTGGGCGTTTAGATTCAGATACCACTGGCCT
TGTGCTTATTACCGATGACGGTCGATGGTCATACAATATTATTACGCCATCAAAGAAATGCGAGAAAGTATATCGAGTTG
GTTTACGTAATATAATTACCAGTGAGGCAATAATCAAGATCGAGTTAGGTGTGCAACTGCAAGGGGAAGTAGAGTTAACA
AGGCCTGCTAAATTGAAGATTATTACAGAGAGAGAAGTATTACTGACCATTATTGAAGGTAAGTTCCATCAGGTAAAACG
TATGTTTTCTGCTGTTGGAAATAAAGTGATTTTATTGCATAGAGAGCAGTTCGGAGAGATCAGTTTGGATGTAAATGAAG
GTGAATGGCGTTATTTAACTGATTATGAAGTTAACTCATTTGAGTATTAA

Upstream 100 bases:

>100_bases
AAACTAAAAAGGCGATCTTCGGATCGCCTTTTTTGATCGTTGCTTATTTATAAAGAGAAAGGATAATAGGATGATAATAA
GTCCTTATAGTGTGAGCCTT

Downstream 100 bases:

>100_bases
GTAAGGTGCCATTGAAGGCACCTCATCAATAAACGTTATTTTTTCCTAAATGGTTGAGTCAACATTTTGTCTAACCGTTG
CTCTTTTTCGCTTCTAAATA

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MRLDKFVCKSTTLTKNEALECIAEGRVKVNAKIVTVESTQVHENNHVTLNGDLLTARPFRYLLMNKPANTICSNIDEAYP
SLFNYIKEGNNSELHIAGRLDSDTTGLVLITDDGRWSYNIITPSKKCEKVYRVGLRNIITSEAIIKIELGVQLQGEVELT
RPAKLKIITEREVLLTIIEGKFHQVKRMFSAVGNKVILLHREQFGEISLDVNEGEWRYLTDYEVNSFEY

Sequences:

>Translated_229_residues
MRLDKFVCKSTTLTKNEALECIAEGRVKVNAKIVTVESTQVHENNHVTLNGDLLTARPFRYLLMNKPANTICSNIDEAYP
SLFNYIKEGNNSELHIAGRLDSDTTGLVLITDDGRWSYNIITPSKKCEKVYRVGLRNIITSEAIIKIELGVQLQGEVELT
RPAKLKIITEREVLLTIIEGKFHQVKRMFSAVGNKVILLHREQFGEISLDVNEGEWRYLTDYEVNSFEY
>Mature_229_residues
MRLDKFVCKSTTLTKNEALECIAEGRVKVNAKIVTVESTQVHENNHVTLNGDLLTARPFRYLLMNKPANTICSNIDEAYP
SLFNYIKEGNNSELHIAGRLDSDTTGLVLITDDGRWSYNIITPSKKCEKVYRVGLRNIITSEAIIKIELGVQLQGEVELT
RPAKLKIITEREVLLTIIEGKFHQVKRMFSAVGNKVILLHREQFGEISLDVNEGEWRYLTDYEVNSFEY

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=43.4782608695652, Blast_Score=190, Evalue=5e-50,
Organism=Escherichia coli, GI1787524, Length=240, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=28.9473684210526, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=32.4324324324324, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26107; Mature: 26107

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFVCKSTTLTKNEALECIAEGRVKVNAKIVTVESTQVHENNHVTLNGDLLTARPFR
CCCCHHHHCCCCCCHHHHHHHHHCCEEEEEEEEEEEECEEEECCCEEEECCCEEECCCEE
YLLMNKPANTICSNIDEAYPSLFNYIKEGNNSELHIAGRLDSDTTGLVLITDDGRWSYNI
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEE
ITPSKKCEKVYRVGLRNIITSEAIIKIELGVQLQGEVELTRPAKLKIITEREVLLTIIEG
ECCCHHHHHHHHHHHHHHHCCCEEEEEEECEEEECCEEEECCCEEEEEECCEEEEEEECC
KFHQVKRMFSAVGNKVILLHREQFGEISLDVNEGEWRYLTDYEVNSFEY
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MRLDKFVCKSTTLTKNEALECIAEGRVKVNAKIVTVESTQVHENNHVTLNGDLLTARPFR
CCCCHHHHCCCCCCHHHHHHHHHCCEEEEEEEEEEEECEEEECCCEEEECCCEEECCCEE
YLLMNKPANTICSNIDEAYPSLFNYIKEGNNSELHIAGRLDSDTTGLVLITDDGRWSYNI
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEE
ITPSKKCEKVYRVGLRNIITSEAIIKIELGVQLQGEVELTRPAKLKIITEREVLLTIIEG
ECCCHHHHHHHHHHHHHHHCCCEEEEEEECEEEECCEEEECCCEEEEEECCEEEEEEECC
KFHQVKRMFSAVGNKVILLHREQFGEISLDVNEGEWRYLTDYEVNSFEY
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]