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The map label for this gene is fumB [H]

Identifier: 209694876

GI number: 209694876

Start: 1462671

End: 1464215

Strand: Direct

Name: fumB [H]

Synonym: VSAL_I1347

Alternate gene names: 209694876

Gene position: 1462671-1464215 (Clockwise)

Preceding gene: 209694870

Following gene: 209694879

Centisome position: 43.99

GC content: 45.11

Gene sequence:

>1545_bases
ATGACGAACGTTAATAAAGAAATGGTTGCTCAAACGAGAACAACTATTCGTAATGAAGATGTGATCAGCTCTGTTGCTGA
TGCACTGCAGTATATTTCTTACTACCACCCACTTGATTTCGTTCAAGCGCTTGAAAAAGCCTACCATAGAGAACAGAGTC
AAGCGGCTAAAGACGCAATTGCTCAAATATTAATTAATTCACGTATGTCAGCAGAAGGGCGTCGTCCTATTTGTCAGGAT
ACGGGGATTGTGACTTGTTTCGTTAATATTGGTATGAACGTACAGTGGGAAGGGGATTTAACCGTTCAACAAATGATCGA
TGAAGGTACTCGTCAAGCGTACCTTAACCCAGATAATCCACTACGTGCATCAATACTGTCTGATCCTGCTGGTAAACGTA
TTAATACGAAAGACAATACGCCATCGGTCGTGCACATCAATATGGTTCCGGGTAATACCGTTGAGATTCAAATCGCAGCA
AAAGGCGGCGGTTCTGAAAATAAAACCAAAATGGTGATGCTAAACCCATCAGATGATATTGCGGAATGGGTAGAAAAAAC
CTTGCCATTAATGGGCGCGGGTTGGTGTCCACCAGGTATGCTAGGTATCGGTATTGGTGGTACAGCAGAAAAAGCCGCCG
TGTTAGCGAAAGAATCGTTAATGGGTCACATTGATATTCAAGAATTGATTGACCGTGGTCCAGAGAATGCAGAAGAAGAA
CTGCGTCTTGATATCTTTAATCGTGTGAATAAGCTAGGCATTGGTGCACAAGGTCTTGGCGGATTAACCACGGTTGTTGA
TGTGAAAATAAAATCTGCACCGACACATGCCGCTTCTAAGCCTGTGTGCATGATCCCTAACTGTGCAGCAACACGTCACG
TACATTTTACCCTTGATGGTTCGGGCCCTGCTGATCTTCAGCCGCCAAAACTGGAAGAGTGGCCAGACATCACATGGGAA
GCGGGCGCGAATACACGTCGTGTGAATCTTGATGGACTGACCAAAGCTGACGTACAAGAATGGCGCACAGGTGAAACGGT
TCTACTAACTGGCAAGATTTTAACCGGTCGTGACGCGGCTCATAAACGTCTGCAAAGCATGATTGAAAGCGGCGAAGGTT
TACCAGAAGGTGTCGATTTTAATGGTAAGTTCATTTATTACGTTGGCCCTGTTGACGCAGTGCGTGACGAAGCCGTTGGT
CCTGCGGGTCCAACGACATCAACACGTATGGATAAGTTTACTGACATGATGCTAGAAACGGGTCTAATGGGAATGATCGG
TAAAGCAGAGCGTGGCCCTGCAACCGTAGCGTCTATTAAAGCCAAAAAAGCGGTTTATTTAATGGCCGTCGGTGGTGCCG
CATATCTCGTATCTAAAGCGATTAAAAAAGCAAGAGTGGTTGCTTTTGAAGAGCTAGGAATGGAAGCGATTTATGAATTT
GATGTGGAAGACATGCCAGTGACTGTTGCGGTTGATTCAACGGGCGCAAATGCACACCAAATTGGTCCCGATACGTGGAA
AGTAAAAATCGCAGAAATGGAATAA

Upstream 100 bases:

>100_bases
GAGTTCTAAAAGAAAATAATAGATGACGATTGCTCACGTCATTACTAAAAAGAACGACATTACATTATTGCCATGGAAGC
ATATATTTTAAGAGGGCCCT

Downstream 100 bases:

>100_bases
GTTAGTAATTTACTAAATAACCTGAATTCTGGATAAAACATGCTTTAAGCGAGGATTAGTTCAAATTCACATTCTGATAG
CTTAATTCGTGGCTTTTGTT

Product: fumarate hydratase class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTNVNKEMVAQTRTTIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREQSQAAKDAIAQILINSRMSAEGRRPICQD
TGIVTCFVNIGMNVQWEGDLTVQQMIDEGTRQAYLNPDNPLRASILSDPAGKRINTKDNTPSVVHINMVPGNTVEIQIAA
KGGGSENKTKMVMLNPSDDIAEWVEKTLPLMGAGWCPPGMLGIGIGGTAEKAAVLAKESLMGHIDIQELIDRGPENAEEE
LRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPADLQPPKLEEWPDITWE
AGANTRRVNLDGLTKADVQEWRTGETVLLTGKILTGRDAAHKRLQSMIESGEGLPEGVDFNGKFIYYVGPVDAVRDEAVG
PAGPTTSTRMDKFTDMMLETGLMGMIGKAERGPATVASIKAKKAVYLMAVGGAAYLVSKAIKKARVVAFEELGMEAIYEF
DVEDMPVTVAVDSTGANAHQIGPDTWKVKIAEME

Sequences:

>Translated_514_residues
MTNVNKEMVAQTRTTIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREQSQAAKDAIAQILINSRMSAEGRRPICQD
TGIVTCFVNIGMNVQWEGDLTVQQMIDEGTRQAYLNPDNPLRASILSDPAGKRINTKDNTPSVVHINMVPGNTVEIQIAA
KGGGSENKTKMVMLNPSDDIAEWVEKTLPLMGAGWCPPGMLGIGIGGTAEKAAVLAKESLMGHIDIQELIDRGPENAEEE
LRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPADLQPPKLEEWPDITWE
AGANTRRVNLDGLTKADVQEWRTGETVLLTGKILTGRDAAHKRLQSMIESGEGLPEGVDFNGKFIYYVGPVDAVRDEAVG
PAGPTTSTRMDKFTDMMLETGLMGMIGKAERGPATVASIKAKKAVYLMAVGGAAYLVSKAIKKARVVAFEELGMEAIYEF
DVEDMPVTVAVDSTGANAHQIGPDTWKVKIAEME
>Mature_513_residues
TNVNKEMVAQTRTTIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREQSQAAKDAIAQILINSRMSAEGRRPICQDT
GIVTCFVNIGMNVQWEGDLTVQQMIDEGTRQAYLNPDNPLRASILSDPAGKRINTKDNTPSVVHINMVPGNTVEIQIAAK
GGGSENKTKMVMLNPSDDIAEWVEKTLPLMGAGWCPPGMLGIGIGGTAEKAAVLAKESLMGHIDIQELIDRGPENAEEEL
RLDIFNRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDGSGPADLQPPKLEEWPDITWEA
GANTRRVNLDGLTKADVQEWRTGETVLLTGKILTGRDAAHKRLQSMIESGEGLPEGVDFNGKFIYYVGPVDAVRDEAVGP
AGPTTSTRMDKFTDMMLETGLMGMIGKAERGPATVASIKAKKAVYLMAVGGAAYLVSKAIKKARVVAFEELGMEAIYEFD
VEDMPVTVAVDSTGANAHQIGPDTWKVKIAEME

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=30.8510638297872, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787897, Length=471, Percent_Identity=31.8471337579618, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789442, Length=301, Percent_Identity=29.5681063122924, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789443, Length=167, Percent_Identity=34.7305389221557, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55555; Mature: 55424

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVNKEMVAQTRTTIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREQSQAAKDAI
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AQILINSRMSAEGRRPICQDTGIVTCFVNIGMNVQWEGDLTVQQMIDEGTRQAYLNPDNP
HHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCCCEEECCCCCC
LRASILSDPAGKRINTKDNTPSVVHINMVPGNTVEIQIAAKGGGSENKTKMVMLNPSDDI
CCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHH
AEWVEKTLPLMGAGWCPPGMLGIGIGGTAEKAAVLAKESLMGHIDIQELIDRGPENAEEE
HHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHH
LRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDG
HHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECC
SGPADLQPPKLEEWPDITWEAGANTRRVNLDGLTKADVQEWRTGETVLLTGKILTGRDAA
CCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCHHH
HKRLQSMIESGEGLPEGVDFNGKFIYYVGPVDAVRDEAVGPAGPTTSTRMDKFTDMMLET
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
GLMGMIGKAERGPATVASIKAKKAVYLMAVGGAAYLVSKAIKKARVVAFEELGMEAIYEF
CHHHHCCCCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHEEEC
DVEDMPVTVAVDSTGANAHQIGPDTWKVKIAEME
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TNVNKEMVAQTRTTIRNEDVISSVADALQYISYYHPLDFVQALEKAYHREQSQAAKDAI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AQILINSRMSAEGRRPICQDTGIVTCFVNIGMNVQWEGDLTVQQMIDEGTRQAYLNPDNP
HHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCCCEEECCCCCC
LRASILSDPAGKRINTKDNTPSVVHINMVPGNTVEIQIAAKGGGSENKTKMVMLNPSDDI
CCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHH
AEWVEKTLPLMGAGWCPPGMLGIGIGGTAEKAAVLAKESLMGHIDIQELIDRGPENAEEE
HHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHH
LRLDIFNRVNKLGIGAQGLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDG
HHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEEECC
SGPADLQPPKLEEWPDITWEAGANTRRVNLDGLTKADVQEWRTGETVLLTGKILTGRDAA
CCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEEECCCHHH
HKRLQSMIESGEGLPEGVDFNGKFIYYVGPVDAVRDEAVGPAGPTTSTRMDKFTDMMLET
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
GLMGMIGKAERGPATVASIKAKKAVYLMAVGGAAYLVSKAIKKARVVAFEELGMEAIYEF
CHHHHCCCCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHEEEC
DVEDMPVTVAVDSTGANAHQIGPDTWKVKIAEME
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]