Click here to switch to the map view.

The map label for this gene is sbcD [H]

Identifier: 209694863

GI number: 209694863

Start: 1444489

End: 1445733

Strand: Direct

Name: sbcD [H]

Synonym: VSAL_I1332

Alternate gene names: 209694863

Gene position: 1444489-1445733 (Clockwise)

Preceding gene: 209694861

Following gene: 209694864

Centisome position: 43.44

GC content: 41.61

Gene sequence:

>1245_bases
ATGCGTATTTTACATACTTCTGATTGGCACCTAGGTCAAAACTTCTTCACAAAAAGCCGCCGTAATGAACATCAAAAATT
CATTGCTTGGTTACTTGAGCAAGTTCAAGAAAATGTCATTAATGCCGTGATCATTGCAGGAGATGTGTTTGATACAGGCG
CGCCACCAAGTTATGCCCGTGAAATGTACAATCAATTTGTGGTGGAGATGAACAAAGTAAACTGCGAATTAATTGTTCTT
GGCGGTAACCATGATTCGGTATCGACGTTAAATGAATCGAAGCAACTGTTAGCGCATTTAAACGCACGAGTAATCGCGAA
TACCAATGAAGACTTATCAACTCAATTATTAACGCTGCCTAATTCAGATGGCACTGTGGGTGCAATTCTATGTGCAGTGC
CTTTTATTCGCCCTCGTGACGTTGTCACTAGTGTGGCGGGGTCTTCTGGTGTAGAAAAGCAGCAAGCACTCGGCGAAGCC
ATTAAATCTCACTATCATCAGCTATACCAAAAAGCATTAGAATTGAGAGAAGAGTTAGCACTTGATGTGCCTATTATCGC
AACAGGTCATTTAACCGCCCTAGGGGTTAAACAATCCGATTCTGTGCGTGATATTTATATTGGTACTTTGGATGGTTTTG
CCGCTGATGGTTTTCCGCCTGCGGATTACATCGCGCTTGGTCATATTCACCGCCCTCAACTTGTGGCTAAATCGGAACAC
ATCCGTTATTCCGGCTCACCAATTCCATTGAGCTTTGATGAATTAAAATCGCAAAAACAAGTCGTAATGGTCGAGTTTGA
TAAAGATAAAGTGAGTCACATTACGCCAATTAATGTGCCGATATTTCAAGAAATGAAAGCATTGAAAGGCGATTTAGAGA
CCATTGAGCAAGAGCTAGCGCAATTTAAAGAGTGCGAAGAAACGACGTGGTTGTGCATTGAAGTCGAAGTTCAAGATTAC
TTGTCCGATCTACAGCAACGTATTCAAACATTAACTCAAGATTTGAATGTGGAAGTGCTGCAATTGCGTCGAGCGAGAAA
CCGCAACGAGCAAACACTGGCGCAAGAAAAAACAGAAACGCTAGCGGAGTTAACGCCTTTTGATGTATTTACCAAACGTA
TTGAGCAAGAAATATTTGAGACAGAAAAAGAGCAGCAACGCGCAGAGCGAATGACAACCAAATTTAAACAGATTTTGTCA
GAAGTAGAACATAAAGATACTGATCCACAAGAAGGTGATGCATGA

Upstream 100 bases:

>100_bases
AATATTAGGGACGGAATTTGCCATTAATACTGCCACTAGAATGCTTCAAATGGTATAAAAGAACTATTGTGTTTCTTATC
CATCAAACAAGTTGATATTT

Downstream 100 bases:

>100_bases
GAATTTTAAGCCTAAGTTTTTCAAATTTAAACTCATTAAAAGGTGAGTGGAAAATTGATTTCTCAGCGTCGCCCTTTGCT
GAAAATGGATTGTTTGCGAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRILHTSDWHLGQNFFTKSRRNEHQKFIAWLLEQVQENVINAVIIAGDVFDTGAPPSYAREMYNQFVVEMNKVNCELIVL
GGNHDSVSTLNESKQLLAHLNARVIANTNEDLSTQLLTLPNSDGTVGAILCAVPFIRPRDVVTSVAGSSGVEKQQALGEA
IKSHYHQLYQKALELREELALDVPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
IRYSGSPIPLSFDELKSQKQVVMVEFDKDKVSHITPINVPIFQEMKALKGDLETIEQELAQFKECEETTWLCIEVEVQDY
LSDLQQRIQTLTQDLNVEVLQLRRARNRNEQTLAQEKTETLAELTPFDVFTKRIEQEIFETEKEQQRAERMTTKFKQILS
EVEHKDTDPQEGDA

Sequences:

>Translated_414_residues
MRILHTSDWHLGQNFFTKSRRNEHQKFIAWLLEQVQENVINAVIIAGDVFDTGAPPSYAREMYNQFVVEMNKVNCELIVL
GGNHDSVSTLNESKQLLAHLNARVIANTNEDLSTQLLTLPNSDGTVGAILCAVPFIRPRDVVTSVAGSSGVEKQQALGEA
IKSHYHQLYQKALELREELALDVPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
IRYSGSPIPLSFDELKSQKQVVMVEFDKDKVSHITPINVPIFQEMKALKGDLETIEQELAQFKECEETTWLCIEVEVQDY
LSDLQQRIQTLTQDLNVEVLQLRRARNRNEQTLAQEKTETLAELTPFDVFTKRIEQEIFETEKEQQRAERMTTKFKQILS
EVEHKDTDPQEGDA
>Mature_414_residues
MRILHTSDWHLGQNFFTKSRRNEHQKFIAWLLEQVQENVINAVIIAGDVFDTGAPPSYAREMYNQFVVEMNKVNCELIVL
GGNHDSVSTLNESKQLLAHLNARVIANTNEDLSTQLLTLPNSDGTVGAILCAVPFIRPRDVVTSVAGSSGVEKQQALGEA
IKSHYHQLYQKALELREELALDVPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
IRYSGSPIPLSFDELKSQKQVVMVEFDKDKVSHITPINVPIFQEMKALKGDLETIEQELAQFKECEETTWLCIEVEVQDY
LSDLQQRIQTLTQDLNVEVLQLRRARNRNEQTLAQEKTETLAELTPFDVFTKRIEQEIFETEKEQQRAERMTTKFKQILS
EVEHKDTDPQEGDA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=405, Percent_Identity=52.3456790123457, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46846; Mature: 46846

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGQNFFTKSRRNEHQKFIAWLLEQVQENVINAVIIAGDVFDTGAPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
EMYNQFVVEMNKVNCELIVLGGNHDSVSTLNESKQLLAHLNARVIANTNEDLSTQLLTLP
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECC
NSDGTVGAILCAVPFIRPRDVVTSVAGSSGVEKQQALGEAIKSHYHQLYQKALELREELA
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDVPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
CCCCEEECCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHEECCCCCCHHHHCCCC
IRYSGSPIPLSFDELKSQKQVVMVEFDKDKVSHITPINVPIFQEMKALKGDLETIEQELA
EEECCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QFKECEETTWLCIEVEVQDYLSDLQQRIQTLTQDLNVEVLQLRRARNRNEQTLAQEKTET
HHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
LAELTPFDVFTKRIEQEIFETEKEQQRAERMTTKFKQILSEVEHKDTDPQEGDA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGQNFFTKSRRNEHQKFIAWLLEQVQENVINAVIIAGDVFDTGAPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
EMYNQFVVEMNKVNCELIVLGGNHDSVSTLNESKQLLAHLNARVIANTNEDLSTQLLTLP
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECC
NSDGTVGAILCAVPFIRPRDVVTSVAGSSGVEKQQALGEAIKSHYHQLYQKALELREELA
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDVPIIATGHLTALGVKQSDSVRDIYIGTLDGFAADGFPPADYIALGHIHRPQLVAKSEH
CCCCEEECCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHEECCCCCCHHHHCCCC
IRYSGSPIPLSFDELKSQKQVVMVEFDKDKVSHITPINVPIFQEMKALKGDLETIEQELA
EEECCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QFKECEETTWLCIEVEVQDYLSDLQQRIQTLTQDLNVEVLQLRRARNRNEQTLAQEKTET
HHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
LAELTPFDVFTKRIEQEIFETEKEQQRAERMTTKFKQILSEVEHKDTDPQEGDA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]