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The map label for this gene is ushA [H]

Identifier: 209694382

GI number: 209694382

Start: 887932

End: 889593

Strand: Direct

Name: ushA [H]

Synonym: VSAL_I0800

Alternate gene names: 209694382

Gene position: 887932-889593 (Clockwise)

Preceding gene: 209694376

Following gene: 209694385

Centisome position: 26.7

GC content: 40.79

Gene sequence:

>1662_bases
ATGATGGAACGTCTTTCTCTTAAAACAGCGATTGCTGCCGCATTACTTACTACATTGGCTGGTTGCGCTACAACGTCTGA
ACATGCGTGGCAAGAAGACACTACGTATAAGTTAACTGTGCTACATACTAACGATAACCATGGCCGCTTTTGGCAAAATA
AATATGGTGAGTACGGTATGGCAGCGCGTAAGACGTTGATTGATGAATTACGTGCCGATGTTCAAGCTGAAGGGGGGAGT
GTTTTACTTCTTTCTGGTGGTGATATTAATACCGGTGTACCTGAGTCTGATTTACAAGATGCAGAGCCTGATTTTAAAGG
CATGAATAAAATTGGATATGATGCGATTGCATTAGGTAACCATGAATTTGATAACCCATTAGATGTGCTTCAAAAGCAAA
TAGATTGGGCTAAATTCCCAATGCTGTCTGCCAACATTTATGATAAAGAAACTGGTGAGCGCATGTATCAAGCTTACGAG
ATTTTTGAAAAGCAAGGCATTAAAATTGCGGTTATTGGTTTCACGACAGAAGACACGGCTAAAATTGGTAACCCTGAATA
CATCGGTGGTATTGATTTCCGAGATCCAAAAGAAGAAGCAAAGAAAGTCATTGCTGAACTGAATGAATCAGAAAAACCCG
ATCTTATTTTTGCAGTTACTCACATGGGTCACTATGAAAATGGTCAACGTGGTATTAATGCACCAGGTGATGTAGCACTT
GCTCGTTCTCTTGAATTAGGTGAGTTGGATATGATTGTGGGTGGCCACTCACAAGAGCCTGTTTGTATGGAAGGGGCGAA
CGTTGTTAAACAAAACTTTAAGCCTGGTGACGAATGTCAACCTGATCAACAAAATGGCACTTGGATCGTTCAAGCTCATG
AATGGGGCAAGTACGTAGGTCGTGCTGATTTTGAATTCCGTAATGGTGAATTGGAAATGGTGAGTTATAACCTAATCCCG
GTTAACTTGAAGAAGAAAATAAAAGTTGATGGCAAAAGTGAGCGTGTCTTTATTCAAGATGAAATTGTGCAAGATCCTGA
GTTATTGGCTTTCCTAACGCCTTACCAAGAAAAAGGTCAAGGCCAGTTGAATGTGAAAATTGCAGAAACGAATGGAAAGT
TAGAAGGTGACCGTAATGTGGTTCGTTTTGAGCAGACTAACCTTGGGCGTTTAATTGCGACTTCTCACATGGTTCGTGCA
AAAGCCGATTTTGCGGTAATGAATTCGGGTGGTGTACGTGATTCGATTGAAATGGGTGATGTTACTTATAAAGATGTTCT
AACGGTGCAGCCATTTGGTAATATTGTTAGTTATGTTGATATGAATCGCGCTGAAGTATTAGATTACCTAAATGTGGTGG
CGACTAAACCAATCGATTCTGGTGCCTATGCTCAGTTCGCAGGTATTAAAATGACCGTTGAAGCCGGTAAAGTATCTAAC
GTATTCATTGGTGGTAAGCAACTGCGTTTAGACAAAACGTACCGTTTTACTATTCCAAGCTACAACGCCGCGGGTGGTGA
TGGTTACCCTAAAGTGTCGGGTCATTCGGGTTATGTAAATACGGGCTTTGTTGATGCCGAAGTATTAAAAGAGTTTTTAG
AAACAAATAGCCCAATTGATGTGAATGAATTCGCACCACGTGGTGAAATTGTTTATAAGTAA

Upstream 100 bases:

>100_bases
GTGAAAAGCAATTCTTTTAAACTCGGTCAGGTTATTTGATTATTAAAGTGGTTTTCCCTTTTTTGATCTAACTTATCCCC
TTGAATTTAAATGGTGAAAC

Downstream 100 bases:

>100_bases
TAGATAAAAAGTAAACTGATCCCCTCAGATTGTTATTTACTGAGGGGATTTAGTTGTAAATTCAACGTCTTTGCTGAATA
AATGACCGAACAAATTAAAA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MMERLSLKTAIAAALLTTLAGCATTSEHAWQEDTTYKLTVLHTNDNHGRFWQNKYGEYGMAARKTLIDELRADVQAEGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNHEFDNPLDVLQKQIDWAKFPMLSANIYDKETGERMYQAYE
IFEKQGIKIAVIGFTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELNESEKPDLIFAVTHMGHYENGQRGINAPGDVAL
ARSLELGELDMIVGGHSQEPVCMEGANVVKQNFKPGDECQPDQQNGTWIVQAHEWGKYVGRADFEFRNGELEMVSYNLIP
VNLKKKIKVDGKSERVFIQDEIVQDPELLAFLTPYQEKGQGQLNVKIAETNGKLEGDRNVVRFEQTNLGRLIATSHMVRA
KADFAVMNSGGVRDSIEMGDVTYKDVLTVQPFGNIVSYVDMNRAEVLDYLNVVATKPIDSGAYAQFAGIKMTVEAGKVSN
VFIGGKQLRLDKTYRFTIPSYNAAGGDGYPKVSGHSGYVNTGFVDAEVLKEFLETNSPIDVNEFAPRGEIVYK

Sequences:

>Translated_553_residues
MMERLSLKTAIAAALLTTLAGCATTSEHAWQEDTTYKLTVLHTNDNHGRFWQNKYGEYGMAARKTLIDELRADVQAEGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNHEFDNPLDVLQKQIDWAKFPMLSANIYDKETGERMYQAYE
IFEKQGIKIAVIGFTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELNESEKPDLIFAVTHMGHYENGQRGINAPGDVAL
ARSLELGELDMIVGGHSQEPVCMEGANVVKQNFKPGDECQPDQQNGTWIVQAHEWGKYVGRADFEFRNGELEMVSYNLIP
VNLKKKIKVDGKSERVFIQDEIVQDPELLAFLTPYQEKGQGQLNVKIAETNGKLEGDRNVVRFEQTNLGRLIATSHMVRA
KADFAVMNSGGVRDSIEMGDVTYKDVLTVQPFGNIVSYVDMNRAEVLDYLNVVATKPIDSGAYAQFAGIKMTVEAGKVSN
VFIGGKQLRLDKTYRFTIPSYNAAGGDGYPKVSGHSGYVNTGFVDAEVLKEFLETNSPIDVNEFAPRGEIVYK
>Mature_553_residues
MMERLSLKTAIAAALLTTLAGCATTSEHAWQEDTTYKLTVLHTNDNHGRFWQNKYGEYGMAARKTLIDELRADVQAEGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGNHEFDNPLDVLQKQIDWAKFPMLSANIYDKETGERMYQAYE
IFEKQGIKIAVIGFTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELNESEKPDLIFAVTHMGHYENGQRGINAPGDVAL
ARSLELGELDMIVGGHSQEPVCMEGANVVKQNFKPGDECQPDQQNGTWIVQAHEWGKYVGRADFEFRNGELEMVSYNLIP
VNLKKKIKVDGKSERVFIQDEIVQDPELLAFLTPYQEKGQGQLNVKIAETNGKLEGDRNVVRFEQTNLGRLIATSHMVRA
KADFAVMNSGGVRDSIEMGDVTYKDVLTVQPFGNIVSYVDMNRAEVLDYLNVVATKPIDSGAYAQFAGIKMTVEAGKVSN
VFIGGKQLRLDKTYRFTIPSYNAAGGDGYPKVSGHSGYVNTGFVDAEVLKEFLETNSPIDVNEFAPRGEIVYK

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=185, Percent_Identity=31.3513513513514, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1786687, Length=548, Percent_Identity=61.6788321167883, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=497, Percent_Identity=21.5291750503018, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922446, Length=511, Percent_Identity=26.2230919765166, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24654424, Length=511, Percent_Identity=26.2230919765166, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922444, Length=564, Percent_Identity=23.0496453900709, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161076508, Length=508, Percent_Identity=26.3779527559055, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652512, Length=508, Percent_Identity=26.3779527559055, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19921980, Length=508, Percent_Identity=26.3779527559055, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573524, Length=538, Percent_Identity=24.907063197026, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641187, Length=560, Percent_Identity=22.5, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI221329836, Length=557, Percent_Identity=21.3644524236984, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 61188; Mature: 61188

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMERLSLKTAIAAALLTTLAGCATTSEHAWQEDTTYKLTVLHTNDNHGRFWQNKYGEYGM
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHCCCCCCH
AARKTLIDELRADVQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGN
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
HEFDNPLDVLQKQIDWAKFPMLSANIYDKETGERMYQAYEIFEKQGIKIAVIGFTTEDTA
CCCCCHHHHHHHHHCHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
KIGNPEYIGGIDFRDPKEEAKKVIAELNESEKPDLIFAVTHMGHYENGQRGINAPGDVAL
HCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
ARSLELGELDMIVGGHSQEPVCMEGANVVKQNFKPGDECQPDQQNGTWIVQAHEWGKYVG
EECCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
RADFEFRNGELEMVSYNLIPVNLKKKIKVDGKSERVFIQDEIVQDPELLAFLTPYQEKGQ
CCCEEEECCEEEEEEEEEEEECCEEEEEECCCCCEEEEEHHHCCCCCEEEEECCHHHCCC
GQLNVKIAETNGKLEGDRNVVRFEQTNLGRLIATSHMVRAKADFAVMNSGGVRDSIEMGD
CEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCEEEECCCCCCCCCCCCC
VTYKDVLTVQPFGNIVSYVDMNRAEVLDYLNVVATKPIDSGAYAQFAGIKMTVEAGKVSN
EEHHHEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCEE
VFIGGKQLRLDKTYRFTIPSYNAAGGDGYPKVSGHSGYVNTGFVDAEVLKEFLETNSPID
EEECCEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNEFAPRGEIVYK
HHHCCCCCCEEEC
>Mature Secondary Structure
MMERLSLKTAIAAALLTTLAGCATTSEHAWQEDTTYKLTVLHTNDNHGRFWQNKYGEYGM
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHCCCCCCH
AARKTLIDELRADVQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAIALGN
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
HEFDNPLDVLQKQIDWAKFPMLSANIYDKETGERMYQAYEIFEKQGIKIAVIGFTTEDTA
CCCCCHHHHHHHHHCHHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
KIGNPEYIGGIDFRDPKEEAKKVIAELNESEKPDLIFAVTHMGHYENGQRGINAPGDVAL
HCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCE
ARSLELGELDMIVGGHSQEPVCMEGANVVKQNFKPGDECQPDQQNGTWIVQAHEWGKYVG
EECCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
RADFEFRNGELEMVSYNLIPVNLKKKIKVDGKSERVFIQDEIVQDPELLAFLTPYQEKGQ
CCCEEEECCEEEEEEEEEEEECCEEEEEECCCCCEEEEEHHHCCCCCEEEEECCHHHCCC
GQLNVKIAETNGKLEGDRNVVRFEQTNLGRLIATSHMVRAKADFAVMNSGGVRDSIEMGD
CEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHEECCCEEEECCCCCCCCCCCCC
VTYKDVLTVQPFGNIVSYVDMNRAEVLDYLNVVATKPIDSGAYAQFAGIKMTVEAGKVSN
EEHHHEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCEE
VFIGGKQLRLDKTYRFTIPSYNAAGGDGYPKVSGHSGYVNTGFVDAEVLKEFLETNSPID
EEECCEEEEEECEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNEFAPRGEIVYK
HHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA