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The map label for this gene is iscS [H]

Identifier: 209694306

GI number: 209694306

Start: 802296

End: 803516

Strand: Direct

Name: iscS [H]

Synonym: VSAL_I0717

Alternate gene names: 209694306

Gene position: 802296-803516 (Clockwise)

Preceding gene: 209694305

Following gene: 209694307

Centisome position: 24.13

GC content: 41.2

Gene sequence:

>1221_bases
ATGAAAATGAAACTGCCAATTTACTTTGATTATTCGGCGACTTGTCCTGTTGATAAGCGCGTAGCCGATAAAATGGTTCA
ACATATGACTATGGATGGATGCTTTGGTAACCCAGCATCACGTTCACACCGTTACGGATGGCAGGCTGAAGAAGCAGTAG
ATACTGCTCGTGACAATATTGCTGCTTTATTAAATGCCGATCCACGTGAGATCGTATTTACATCAGGCGCTACAGAATCA
GATAACCTTGCAATTAAAGGTGCTGCGCATTTCTATCATAAGAAAGGTAAGCACGTAATTACATGTAAAACTGAGCATAA
AGCGGTTCTTGATCCATGTCGTCAACTTGAGCGCGAAGGCTATGAAGTGACGTACTTAGATCCAGAATCAAATGGTTTGG
TTGATTTCGCTAAATTAGAAGCAGCAATGCGTGAAGACACGGTTCTTGTTTCTATTATGCACGTAAATAATGAAATTGGT
GTGATTCAAGATATCACGGCAATCGGTGAACTTTGTCGTTCACGTAAAATTGTTTTCCATGTAGATGCGGCGCAATCAGC
GGGTAAAATCCCACTTGATGTACAAGAAGTGAAAGTAGATTTAATCTCACTTTCTGCTCATAAAATGTATGGCCCTAAAG
GCATTGGTGCATTGTACGTTCGTCGTAAACCACGTATTCGTTTAGAAGCACAAATGCACGGTGGTGGCCACGAACGAGGT
TTCCGTTCTGGTACATTAGCAACTCACCAAATTGTTGGTATGGGTGAAGCCTGTCGTATTGCAAAAGAAGAGATGCAAAA
AGATTACGACCATTGCCTAGCAATGCGTGATCGTCTTCTTGATGGTGTTAAAGATATGGAAGCGGTACACATTAATGGTG
ATTTAGACCAACGTGTACCACATAACTTAAACATCAGCTTTGAGTTTGTTGAAGGTGAATCATTGCTAATGGCATTGAAA
GATCTTGCTGTTTCTTCTGGTTCAGCATGTACTTCAGCGAGTCTTGAACCATCGTATGTATTGCGTGCATTAGGCTTGAA
TGATGAATTAGCACACAGTTCAATTCGTTTCTCTTTTGGTCGTTATACAACGGCAGAAGACATTGATCATGCAATTACAC
AAATTAGCCAGGCAGTAAATAAACTGCGTGATATGTCTCCGTTATGGGATATGTACAAAGAAGGCATCGACTTAAACACG
GTTGAGTGGGCACACCACTAA

Upstream 100 bases:

>100_bases
GATGCAGCCTTAAATAATGGGTTAGTAACGAATTCAAAATTAACAAATTCTGTGGGTGTTAATGTCCGCTTTTGACGGTC
AAACGTCTGTATTCTCGGAG

Downstream 100 bases:

>100_bases
ACACGGACGTTTCAGAATTTAGAGGTATTTATCATGGCTTATAGCGAAAAAGTAATCGATCACTACGAAAACCCACGTAA
CGTAGGTTCATTTGAAAAAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKMKLPIYFDYSATCPVDKRVADKMVQHMTMDGCFGNPASRSHRYGWQAEEAVDTARDNIAALLNADPREIVFTSGATES
DNLAIKGAAHFYHKKGKHVITCKTEHKAVLDPCRQLEREGYEVTYLDPESNGLVDFAKLEAAMREDTVLVSIMHVNNEIG
VIQDITAIGELCRSRKIVFHVDAAQSAGKIPLDVQEVKVDLISLSAHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERG
FRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGVKDMEAVHINGDLDQRVPHNLNISFEFVEGESLLMALK
DLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRYTTAEDIDHAITQISQAVNKLRDMSPLWDMYKEGIDLNT
VEWAHH

Sequences:

>Translated_406_residues
MKMKLPIYFDYSATCPVDKRVADKMVQHMTMDGCFGNPASRSHRYGWQAEEAVDTARDNIAALLNADPREIVFTSGATES
DNLAIKGAAHFYHKKGKHVITCKTEHKAVLDPCRQLEREGYEVTYLDPESNGLVDFAKLEAAMREDTVLVSIMHVNNEIG
VIQDITAIGELCRSRKIVFHVDAAQSAGKIPLDVQEVKVDLISLSAHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERG
FRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGVKDMEAVHINGDLDQRVPHNLNISFEFVEGESLLMALK
DLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRYTTAEDIDHAITQISQAVNKLRDMSPLWDMYKEGIDLNT
VEWAHH
>Mature_406_residues
MKMKLPIYFDYSATCPVDKRVADKMVQHMTMDGCFGNPASRSHRYGWQAEEAVDTARDNIAALLNADPREIVFTSGATES
DNLAIKGAAHFYHKKGKHVITCKTEHKAVLDPCRQLEREGYEVTYLDPESNGLVDFAKLEAAMREDTVLVSIMHVNNEIG
VIQDITAIGELCRSRKIVFHVDAAQSAGKIPLDVQEVKVDLISLSAHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERG
FRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGVKDMEAVHINGDLDQRVPHNLNISFEFVEGESLLMALK
DLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRYTTAEDIDHAITQISQAVNKLRDMSPLWDMYKEGIDLNT
VEWAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=55.9701492537313, Blast_Score=497, Evalue=1e-140,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=31.8181818181818, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=74.2574257425743, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=399, Percent_Identity=25.8145363408521, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787970, Length=233, Percent_Identity=29.1845493562232, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=404, Percent_Identity=58.4158415841584, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17533177, Length=335, Percent_Identity=33.4328358208955, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=55.860349127182, Blast_Score=485, Evalue=1e-138,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=55.9701492537313, Blast_Score=478, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45239; Mature: 45239

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKLPIYFDYSATCPVDKRVADKMVQHMTMDGCFGNPASRSHRYGWQAEEAVDTARDNI
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AALLNADPREIVFTSGATESDNLAIKGAAHFYHKKGKHVITCKTEHKAVLDPCRQLEREG
HEEECCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCC
YEVTYLDPESNGLVDFAKLEAAMREDTVLVSIMHVNNEIGVIQDITAIGELCRSRKIVFH
CEEEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEE
VDAAQSAGKIPLDVQEVKVDLISLSAHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERG
EECCCCCCCCCCCHHHHEEEEEEECHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCC
FRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGVKDMEAVHINGDLDQRVP
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCEEEEECCCHHHCCC
HNLNISFEFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFG
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCEEEECC
RYTTAEDIDHAITQISQAVNKLRDMSPLWDMYKEGIDLNTVEWAHH
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKMKLPIYFDYSATCPVDKRVADKMVQHMTMDGCFGNPASRSHRYGWQAEEAVDTARDNI
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AALLNADPREIVFTSGATESDNLAIKGAAHFYHKKGKHVITCKTEHKAVLDPCRQLEREG
HEEECCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCC
YEVTYLDPESNGLVDFAKLEAAMREDTVLVSIMHVNNEIGVIQDITAIGELCRSRKIVFH
CEEEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEE
VDAAQSAGKIPLDVQEVKVDLISLSAHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERG
EECCCCCCCCCCCHHHHEEEEEEECHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCC
FRSGTLATHQIVGMGEACRIAKEEMQKDYDHCLAMRDRLLDGVKDMEAVHINGDLDQRVP
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCEEEEECCCHHHCCC
HNLNISFEFVEGESLLMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFG
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCEEEECC
RYTTAEDIDHAITQISQAVNKLRDMSPLWDMYKEGIDLNTVEWAHH
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA