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The map label for this gene is yfhQ [H]

Identifier: 209694304

GI number: 209694304

Start: 800930

End: 801658

Strand: Direct

Name: yfhQ [H]

Synonym: VSAL_I0715

Alternate gene names: 209694304

Gene position: 800930-801658 (Clockwise)

Preceding gene: 209694302

Following gene: 209694305

Centisome position: 24.09

GC content: 39.51

Gene sequence:

>729_bases
ATGTTAGATAGAATTCGTATTGTCCTTGTTGGTACTAGCCATTCAGGAAACATTGGGTCAGCGGCTCGTGCCATGAAAGT
AATGGGCTTAACTAATATGGTATTAGTTGCGCCTGAATGTGAAGTTGATGGTCAAGCCATCGCATTAGCTGCCGGTGCAA
GTGATATTGCGAATCGAGCGCGTATTGTTAGTACATTAGATGAAGCTATCTCTGATTGTGGTTTAGTTATTGGTTCAAGT
GCTCGTTCAAGAACACTCGAATGGCCAATGTTAGAACCAAGAGAAGCGGGCGTCAAAGCAATAGAAGAAGCGCCGTCTCA
TCCTATTGCGTTTGTATTTGGTCGAGAAAGAGTTGGATTAACCAATGAAGAATTACAGAAATGTCATTTTCATGTATGTA
TTCCAGCAAATCCTGAATACAGCTCATTAAATTTAGCCATGGCAGTGCAAACAATTAGTTATGAAGCGCGTGTTGCTTGG
TTAGATAGTCAGGCGTATAAAGGTGAATCTAAAGAAGCGGAATATCCTTTAAATAACGAATTAGAATTATTCTATGAGCA
CCTTGAAAAAGTGATGATGGATACCGACTTTATTAATAAGGCTCATCCAGGACAAGTAATGAATAAGATGCGCCGCATGT
TTAATCGTGCTCGTCCTGAGATTCATGAATTACGTACATTACGCGGTATATTAACTGCGGTAGAAAGAAGCTCAAATATT
AAAAAATAG

Upstream 100 bases:

>100_bases
AAAAAGCGATTACAGGTTAAAATCTCTGGTATATAAAGAAGAAAGGGATATTGCTTTATGGTAGTATCCGGCGGCTCTTT
TCATCATATGGAAAAATAAC

Downstream 100 bases:

>100_bases
ATGCAATCAGAATACTTGACTAAAATAGTAGGATAAATACTTGACCAAAATGGTCAGGTATGGGAAACTTCATTCCATAC
GAATAGTGTGGGTATGAGGT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLDRIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANRARIVSTLDEAISDCGLVIGSS
ARSRTLEWPMLEPREAGVKAIEEAPSHPIAFVFGRERVGLTNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAW
LDSQAYKGESKEAEYPLNNELELFYEHLEKVMMDTDFINKAHPGQVMNKMRRMFNRARPEIHELRTLRGILTAVERSSNI
KK

Sequences:

>Translated_242_residues
MLDRIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANRARIVSTLDEAISDCGLVIGSS
ARSRTLEWPMLEPREAGVKAIEEAPSHPIAFVFGRERVGLTNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAW
LDSQAYKGESKEAEYPLNNELELFYEHLEKVMMDTDFINKAHPGQVMNKMRRMFNRARPEIHELRTLRGILTAVERSSNI
KK
>Mature_242_residues
MLDRIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANRARIVSTLDEAISDCGLVIGSS
ARSRTLEWPMLEPREAGVKAIEEAPSHPIAFVFGRERVGLTNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAW
LDSQAYKGESKEAEYPLNNELELFYEHLEKVMMDTDFINKAHPGQVMNKMRRMFNRARPEIHELRTLRGILTAVERSSNI
KK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=63.9344262295082, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1790865, Length=177, Percent_Identity=35.0282485875706, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26850; Mature: 26850

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANRA
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHHHH
RIVSTLDEAISDCGLVIGSSARSRTLEWPMLEPREAGVKAIEEAPSHPIAFVFGRERVGL
HHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
TNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAWLDSQAYKGESKEAEYPLNNE
CHHHHHHCEEEEEECCCCCCCCCCCEEEEEHHHHEEEEEEECCHHCCCCCCCCCCCCCCH
LELFYEHLEKVMMDTDFINKAHPGQVMNKMRRMFNRARPEIHELRTLRGILTAVERSSNI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
KK
CC
>Mature Secondary Structure
MLDRIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANRA
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHHHH
RIVSTLDEAISDCGLVIGSSARSRTLEWPMLEPREAGVKAIEEAPSHPIAFVFGRERVGL
HHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
TNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAWLDSQAYKGESKEAEYPLNNE
CHHHHHHCEEEEEECCCCCCCCCCCEEEEEHHHHEEEEEEECCHHCCCCCCCCCCCCCCH
LELFYEHLEKVMMDTDFINKAHPGQVMNKMRRMFNRARPEIHELRTLRGILTAVERSSNI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA