Click here to switch to the map view.

The map label for this gene is truC [H]

Identifier: 209694302

GI number: 209694302

Start: 799022

End: 799813

Strand: Direct

Name: truC [H]

Synonym: VSAL_I0713

Alternate gene names: 209694302

Gene position: 799022-799813 (Clockwise)

Preceding gene: 209694301

Following gene: 209694304

Centisome position: 24.03

GC content: 39.14

Gene sequence:

>792_bases
GTGAACATTAATAAACACAATATAAACACTGAAGCGTTGAGCGAAAATGAAGAAAAACCAAAGCCGATTGAATTAGAGAT
CGTGTATCGTGATGACGATTTGATAGCCATTAATAAACCGGCAGGAATGCTTGTTCACCGCTCGTGGCTTGATAAACATG
AAACCGTATTTGCGATGCAAACATTACGAGATCAAATTGGCCAACATGTTTTTCCATTACACCGCTTAGATAGACCTACA
TCTGGCATTTTGTTTTTTGCGTTATCAAGCGCGGTGGCTGCTCAATTTTCACCGTTATTTGCAAGTCGAGAAATTAAAAA
AACGTATCATGCTATTGTGCGTGGCTGGATAGAAGAGGGGGATATTCTTGATTACCCACTTAAAGAACAATTAGATAAAA
TTGCGGATAAAAAGGCGTCAAAAGACGTTGAGGCAAAAGAAGCAATAACGGAATATAAACCCTTGGCTAACGTTGAATTA
CCCTATTCAACAGGTAAATTCCCAACCAGTCGATATTGCTTGATGGAATTAAAGCCGCATACAGGCAGAAAACATCAGCT
ACGTCGTCACATGGCGCATTTACGCCACCCAATTGTTGGGGATACGACTCATGGCGATAGCAAACATAATCGATTATTTC
AAAATAATTTAGATAGTCACCGTTTATTATTACACGCGTCTGAATTGAGTTTCATTCATCCGAGCACGAATAAGCATGTT
GTAATAAAAGCGAGTGTTGGTGAAAAGTGGAATAAACTAATGGCTGATTTTGGGTGGGAAATTGAGTTGTGA

Upstream 100 bases:

>100_bases
TCTCTATCGCGCCTTATATCGAAGAAGCATTGAAAAATCAAAAAGGAACTACTGAGATCATCGGTATTTGCCAGAATATT
GATAAGTTAGGTAAGATTTT

Downstream 100 bases:

>100_bases
TCGATCATCAATAACATCGAGATAATAAAAAAGGGAAGTGAATTAATTCACTTCCCTTTTTTTAATTCAATTTTCAATCA
ATGACAATTATGTCGTCAAC

Product: tRNA pseudouridine synthase C

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNINKHNINTEALSENEEKPKPIELEIVYRDDDLIAINKPAGMLVHRSWLDKHETVFAMQTLRDQIGQHVFPLHRLDRPT
SGILFFALSSAVAAQFSPLFASREIKKTYHAIVRGWIEEGDILDYPLKEQLDKIADKKASKDVEAKEAITEYKPLANVEL
PYSTGKFPTSRYCLMELKPHTGRKHQLRRHMAHLRHPIVGDTTHGDSKHNRLFQNNLDSHRLLLHASELSFIHPSTNKHV
VIKASVGEKWNKLMADFGWEIEL

Sequences:

>Translated_263_residues
MNINKHNINTEALSENEEKPKPIELEIVYRDDDLIAINKPAGMLVHRSWLDKHETVFAMQTLRDQIGQHVFPLHRLDRPT
SGILFFALSSAVAAQFSPLFASREIKKTYHAIVRGWIEEGDILDYPLKEQLDKIADKKASKDVEAKEAITEYKPLANVEL
PYSTGKFPTSRYCLMELKPHTGRKHQLRRHMAHLRHPIVGDTTHGDSKHNRLFQNNLDSHRLLLHASELSFIHPSTNKHV
VIKASVGEKWNKLMADFGWEIEL
>Mature_263_residues
MNINKHNINTEALSENEEKPKPIELEIVYRDDDLIAINKPAGMLVHRSWLDKHETVFAMQTLRDQIGQHVFPLHRLDRPT
SGILFFALSSAVAAQFSPLFASREIKKTYHAIVRGWIEEGDILDYPLKEQLDKIADKKASKDVEAKEAITEYKPLANVEL
PYSTGKFPTSRYCLMELKPHTGRKHQLRRHMAHLRHPIVGDTTHGDSKHNRLFQNNLDSHRLLLHASELSFIHPSTNKHV
VIKASVGEKWNKLMADFGWEIEL

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI17158025, Length=221, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI221316667, Length=198, Percent_Identity=32.3232323232323, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI23308689, Length=182, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221316663, Length=222, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=56.1702127659575, Blast_Score=286, Evalue=7e-79,
Organism=Escherichia coli, GI1787327, Length=246, Percent_Identity=30.0813008130081, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786244, Length=231, Percent_Identity=32.034632034632, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788946, Length=270, Percent_Identity=28.1481481481481, Blast_Score=93, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324506, Length=189, Percent_Identity=31.2169312169312, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320168, Length=196, Percent_Identity=30.1020408163265, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321608, Length=203, Percent_Identity=31.5270935960591, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583449, Length=186, Percent_Identity=30.1075268817204, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583447, Length=186, Percent_Identity=30.1075268817204, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583445, Length=186, Percent_Identity=30.1075268817204, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921090, Length=179, Percent_Identity=29.0502793296089, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 30259; Mature: 30259

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINKHNINTEALSENEEKPKPIELEIVYRDDDLIAINKPAGMLVHRSWLDKHETVFAMQ
CCCCCCCCCHHHHCCCCCCCCCEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
TLRDQIGQHVFPLHRLDRPTSGILFFALSSAVAAQFSPLFASREIKKTYHAIVRGWIEEG
HHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DILDYPLKEQLDKIADKKASKDVEAKEAITEYKPLANVELPYSTGKFPTSRYCLMELKPH
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCC
TGRKHQLRRHMAHLRHPIVGDTTHGDSKHNRLFQNNLDSHRLLLHASELSFIHPSTNKHV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCEEECCCCCCEE
VIKASVGEKWNKLMADFGWEIEL
EEEECCCHHHHHHHHHCCCEECC
>Mature Secondary Structure
MNINKHNINTEALSENEEKPKPIELEIVYRDDDLIAINKPAGMLVHRSWLDKHETVFAMQ
CCCCCCCCCHHHHCCCCCCCCCEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHH
TLRDQIGQHVFPLHRLDRPTSGILFFALSSAVAAQFSPLFASREIKKTYHAIVRGWIEEG
HHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DILDYPLKEQLDKIADKKASKDVEAKEAITEYKPLANVELPYSTGKFPTSRYCLMELKPH
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCC
TGRKHQLRRHMAHLRHPIVGDTTHGDSKHNRLFQNNLDSHRLLLHASELSFIHPSTNKHV
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCEEECCCCCCEE
VIKASVGEKWNKLMADFGWEIEL
EEEECCCHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]