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The map label for this gene is ascG [H]

Identifier: 209694297

GI number: 209694297

Start: 794614

End: 795624

Strand: Direct

Name: ascG [H]

Synonym: VSAL_I0708

Alternate gene names: 209694297

Gene position: 794614-795624 (Clockwise)

Preceding gene: 209694296

Following gene: 209694298

Centisome position: 23.9

GC content: 34.22

Gene sequence:

>1011_bases
ATGGCAACAATTAATGATGTATGTAAGCTTGCTGGTGTTTCTAAAGCGACGGTTTCTCGAGTAATGAATGGGAAAGGGCC
GGTAAAGGAAGAAACCAAAAAACTGATTTTTGATGCAATGGAAACATTGAACTATAAACCGAGCCTCGTCGCTCAAGCAT
TGGCAACAAATAGTTCAAATAGCATTGGTCTTATTGTCTCTTTCTTTGATGGTTATTATTTTGGTATTTTACTCAAAGAA
GCAACAAGAATTGCAGAAGAATCAGGTAAGCAGTTAATTGTTACTGATGGTCATAACCAACTTGAAACCGAAATAGAAGC
GATTAATTCATTAGTTGCTAGAAAGTGTGATGCTATTTTAATTTATAGCCGAACCATGACAGCGACTGATTATTGTAAGA
TCCAAGAAACACTGCCTATTCCTTTAGTTGTTATTAATAGAACATTACCTGATGGGTTAGGGCATGCTGTTGCTTTTGAT
CAATATGATGCAAGTCGAATGGCAATAGAGCATCTTATTGAATTAAAACATAAAAATATTGCAATTATTACAATTAGTTT
GGCTTCAAGTACCGGACAAATAAGATTACAAGGCGCGAAAGAAACACTAGAAAAACATGGTGTACATTACGATGAGAGCT
TAACCATCGTGAAGCATGGTAAGTTGGAAGATGGTTATCAAGCATGCAAAGAATTATTGAGTAAATCAGATAAAATTACC
GCTATATTTTGTTTTAATGATCATTTAGCTCTAGGGGCAATAAAGGCATTAAAAGAAATGAACCTGCGAGTACCTGAAGA
TATTTCAATTGTAGGTATTGATAATGATGAGATATCCGCGTTCTTATCTCCTGCATTAACAACAATTAATGTTCCAATAC
AAGAAATAACACAACTAGCAATGAATAAAGCATTAGCGTTAATCTCGGATAAAGACATCAAACCAACAACCGAAGTATTA
ACTGGGGAACTTATTATTCGTGATTCTAGTATATATAATAAAAACAAGTAA

Upstream 100 bases:

>100_bases
TGAGATCCATCGAGTTTTGCAAAATAAATAATAAAGTGTATTTAGCGTAATAACTTAGCTATTATTACGCTAGTTCTTTT
TGGGTGTATTTGGTGGGTGA

Downstream 100 bases:

>100_bases
CTAATATAAATATCCGTACGTAGGTATTATTCGTTCGGATTTAACTATTCTGTTTAAAGCACAAAAATAAAATATATATA
AATATAAAAGTCATGTGATT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLNYKPSLVAQALATNSSNSIGLIVSFFDGYYFGILLKE
ATRIAEESGKQLIVTDGHNQLETEIEAINSLVARKCDAILIYSRTMTATDYCKIQETLPIPLVVINRTLPDGLGHAVAFD
QYDASRMAIEHLIELKHKNIAIITISLASSTGQIRLQGAKETLEKHGVHYDESLTIVKHGKLEDGYQACKELLSKSDKIT
AIFCFNDHLALGAIKALKEMNLRVPEDISIVGIDNDEISAFLSPALTTINVPIQEITQLAMNKALALISDKDIKPTTEVL
TGELIIRDSSIYNKNK

Sequences:

>Translated_336_residues
MATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLNYKPSLVAQALATNSSNSIGLIVSFFDGYYFGILLKE
ATRIAEESGKQLIVTDGHNQLETEIEAINSLVARKCDAILIYSRTMTATDYCKIQETLPIPLVVINRTLPDGLGHAVAFD
QYDASRMAIEHLIELKHKNIAIITISLASSTGQIRLQGAKETLEKHGVHYDESLTIVKHGKLEDGYQACKELLSKSDKIT
AIFCFNDHLALGAIKALKEMNLRVPEDISIVGIDNDEISAFLSPALTTINVPIQEITQLAMNKALALISDKDIKPTTEVL
TGELIIRDSSIYNKNK
>Mature_335_residues
ATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLNYKPSLVAQALATNSSNSIGLIVSFFDGYYFGILLKEA
TRIAEESGKQLIVTDGHNQLETEIEAINSLVARKCDAILIYSRTMTATDYCKIQETLPIPLVVINRTLPDGLGHAVAFDQ
YDASRMAIEHLIELKHKNIAIITISLASSTGQIRLQGAKETLEKHGVHYDESLTIVKHGKLEDGYQACKELLSKSDKITA
IFCFNDHLALGAIKALKEMNLRVPEDISIVGIDNDEISAFLSPALTTINVPIQEITQLAMNKALALISDKDIKPTTEVLT
GELIIRDSSIYNKNK

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=37.910447761194, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789202, Length=329, Percent_Identity=32.5227963525836, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=33.030303030303, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=32.1311475409836, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=28.3987915407855, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790369, Length=329, Percent_Identity=29.7872340425532, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=28.0597014925373, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=29.874213836478, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=24.9201277955272, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=25.6410256410256, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787906, Length=297, Percent_Identity=26.5993265993266, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790689, Length=311, Percent_Identity=23.4726688102894, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=22.9559748427673, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36833; Mature: 36702

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLNYKPSLVAQALATNSSN
CCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
SIGLIVSFFDGYYFGILLKEATRIAEESGKQLIVTDGHNQLETEIEAINSLVARKCDAIL
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEE
IYSRTMTATDYCKIQETLPIPLVVINRTLPDGLGHAVAFDQYDASRMAIEHLIELKHKNI
EEECCCCCCHHEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCE
AIITISLASSTGQIRLQGAKETLEKHGVHYDESLTIVKHGKLEDGYQACKELLSKSDKIT
EEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEE
AIFCFNDHLALGAIKALKEMNLRVPEDISIVGIDNDEISAFLSPALTTINVPIQEITQLA
EEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCEEEECCHHHHHHHH
MNKALALISDKDIKPTTEVLTGELIIRDSSIYNKNK
HHHHHHEECCCCCCCHHHHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
ATINDVCKLAGVSKATVSRVMNGKGPVKEETKKLIFDAMETLNYKPSLVAQALATNSSN
CCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
SIGLIVSFFDGYYFGILLKEATRIAEESGKQLIVTDGHNQLETEIEAINSLVARKCDAIL
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEE
IYSRTMTATDYCKIQETLPIPLVVINRTLPDGLGHAVAFDQYDASRMAIEHLIELKHKNI
EEECCCCCCHHEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCE
AIITISLASSTGQIRLQGAKETLEKHGVHYDESLTIVKHGKLEDGYQACKELLSKSDKIT
EEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEE
AIFCFNDHLALGAIKALKEMNLRVPEDISIVGIDNDEISAFLSPALTTINVPIQEITQLA
EEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCEEEECCHHHHHHHH
MNKALALISDKDIKPTTEVLTGELIIRDSSIYNKNK
HHHHHHEECCCCCCCHHHHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]