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The map label for this gene is ispH

Identifier: 209694175

GI number: 209694175

Start: 641077

End: 642021

Strand: Direct

Name: ispH

Synonym: VSAL_I0583

Alternate gene names: 209694175

Gene position: 641077-642021 (Clockwise)

Preceding gene: 209694174

Following gene: 209694176

Centisome position: 19.28

GC content: 40.53

Gene sequence:

>945_bases
ATGAAAATAGTATTAGCGAACCCTCGTGGATTTTGTGCCGGTGTTGATCGTGCCATCAGTATTGTTGAACGAGCGCTTGA
ATTATATAAAGCCCCTATTTATGTACGTCATGAAGTGGTTCATAACCGTTTTGTCGTCGAAGGATTAAAGCAACGCGGTG
CTATTTTTGTTGAAGAGTTAAGTGAAGTTCCTGATGATAATATCGTGATCTTTTCAGCTCACGGAGTATCTCAAGCAGTG
CGTACTGAAGCAAAAGAGCGAGCATTAACGGTATTTGATGCCACTTGTCCATTAGTAACAAAAGTTCATATGGAAGTTGC
TCGTGCAAGTAAAAAGAGCATTGAAGTTGTTTTAATTGGTCACGCTGGTCACCCTGAAGTTGAAGGGACCATGGGACAAT
ATGCGAGTGAAGCTGGTGGAATGTATTTAGTCGAGACACCTGAGGATGTAGAGAAGCTGATTGTTCAAGATCCAAATAAC
CTTCATTATGTCAGCCAAACGACATTATCAGTTGATGAAACCGCAGATGTTATTGATGAATTACGCCGTGTATTTCCAAA
GATCCAAGGCCCGCGTAAAGATGATATCTGTTATGCCACACAAAACCGTCAAGATGCAGTGCGAGATATGGCAGGCCAAG
TTGATGTGATGATCGTTGTGGGGTCTAAAAATTCATCTAATTCAAATCGATTGCGTGAACTGTCTGAAAAATTAGGCACA
ACGAGTTATCTGATTGATTGCCCAGAAGAGCTGAAAGAAGAATGGTTAACAAACCAAGCGAAAGTTGGTGTTACTGCCGG
CGCATCAGCACCTGAAGAATTAGTTAACCAAATCATTGAACAAGTAAAAGTCTTTGGTGGTACGACTGTGGATGAATTAA
AAGGGCGTGAAGAAAACATGTTTTTTGAAGTACCAAAAGAATTGCAAATTAAAACTGTGTCTTAA

Upstream 100 bases:

>100_bases
CGTTACTGTCGATTTTAATCACCCATTATCTGGTCAAGATGTTATCTTTGACGTAGAAATCGTAGCGGTTGGATAACCCA
ACAAAAAGTAGAGATAAAAG

Downstream 100 bases:

>100_bases
AAGTTCGCATAGCGATTCATAATAATTTGAGATAGAGTGAAAGATACAAATTTAAAGGAGTACACGAGAATGGTAAGAGT
CGCAATTGCTGGTGCAGCAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKIVLANPRGFCAGVDRAISIVERALELYKAPIYVRHEVVHNRFVVEGLKQRGAIFVEELSEVPDDNIVIFSAHGVSQAV
RTEAKERALTVFDATCPLVTKVHMEVARASKKSIEVVLIGHAGHPEVEGTMGQYASEAGGMYLVETPEDVEKLIVQDPNN
LHYVSQTTLSVDETADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRDMAGQVDVMIVVGSKNSSNSNRLRELSEKLGT
TSYLIDCPEELKEEWLTNQAKVGVTAGASAPEELVNQIIEQVKVFGGTTVDELKGREENMFFEVPKELQIKTVS

Sequences:

>Translated_314_residues
MKIVLANPRGFCAGVDRAISIVERALELYKAPIYVRHEVVHNRFVVEGLKQRGAIFVEELSEVPDDNIVIFSAHGVSQAV
RTEAKERALTVFDATCPLVTKVHMEVARASKKSIEVVLIGHAGHPEVEGTMGQYASEAGGMYLVETPEDVEKLIVQDPNN
LHYVSQTTLSVDETADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRDMAGQVDVMIVVGSKNSSNSNRLRELSEKLGT
TSYLIDCPEELKEEWLTNQAKVGVTAGASAPEELVNQIIEQVKVFGGTTVDELKGREENMFFEVPKELQIKTVS
>Mature_314_residues
MKIVLANPRGFCAGVDRAISIVERALELYKAPIYVRHEVVHNRFVVEGLKQRGAIFVEELSEVPDDNIVIFSAHGVSQAV
RTEAKERALTVFDATCPLVTKVHMEVARASKKSIEVVLIGHAGHPEVEGTMGQYASEAGGMYLVETPEDVEKLIVQDPNN
LHYVSQTTLSVDETADVIDELRRVFPKIQGPRKDDICYATQNRQDAVRDMAGQVDVMIVVGSKNSSNSNRLRELSEKLGT
TSYLIDCPEELKEEWLTNQAKVGVTAGASAPEELVNQIIEQVKVFGGTTVDELKGREENMFFEVPKELQIKTVS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=67.4193548387097, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_ALISL (B6ENC7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262103.1
- GeneID:   6988501
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0583
- HOGENOM:   HBG335228
- OMA:   MDVPGFY
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34765; Mature: 34765

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLANPRGFCAGVDRAISIVERALELYKAPIYVRHEVVHNRFVVEGLKQRGAIFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SEVPDDNIVIFSAHGVSQAVRTEAKERALTVFDATCPLVTKVHMEVARASKKSIEVVLIG
HHCCCCCEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEE
HAGHPEVEGTMGQYASEAGGMYLVETPEDVEKLIVQDPNNLHYVSQTTLSVDETADVIDE
CCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHH
LRRVFPKIQGPRKDDICYATQNRQDAVRDMAGQVDVMIVVGSKNSSNSNRLRELSEKLGT
HHHHHHHCCCCCCCCCEEECCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
TSYLIDCPEELKEEWLTNQAKVGVTAGASAPEELVNQIIEQVKVFGGTTVDELKGREENM
CEEEEECHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCE
FFEVPKELQIKTVS
EEECCCCCEEEECC
>Mature Secondary Structure
MKIVLANPRGFCAGVDRAISIVERALELYKAPIYVRHEVVHNRFVVEGLKQRGAIFVEEL
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SEVPDDNIVIFSAHGVSQAVRTEAKERALTVFDATCPLVTKVHMEVARASKKSIEVVLIG
HHCCCCCEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEE
HAGHPEVEGTMGQYASEAGGMYLVETPEDVEKLIVQDPNNLHYVSQTTLSVDETADVIDE
CCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHH
LRRVFPKIQGPRKDDICYATQNRQDAVRDMAGQVDVMIVVGSKNSSNSNRLRELSEKLGT
HHHHHHHCCCCCCCCCEEECCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
TSYLIDCPEELKEEWLTNQAKVGVTAGASAPEELVNQIIEQVKVFGGTTVDELKGREENM
CEEEEECHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCE
FFEVPKELQIKTVS
EEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA