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The map label for this gene is lysS

Identifier: 209694158

GI number: 209694158

Start: 621111

End: 622616

Strand: Direct

Name: lysS

Synonym: VSAL_I0566

Alternate gene names: 209694158

Gene position: 621111-622616 (Clockwise)

Preceding gene: 209694157

Following gene: 209694159

Centisome position: 18.68

GC content: 41.97

Gene sequence:

>1506_bases
ATGACTGACCAAGTACAACTAGATGAAAACAAACTGATTGCAGAACGCCGTGGTAAATTAGACCATATCCGTCAAGCATG
TAAGGCTAATGGCCACCCGAATGACTTCCGTCGTGACAGTCTTGCTGCGGATCTTCAATCGGAATTTGGTGAAAAGACCA
AAGAAGAGCTAGAAGAATTAAACCACATTGTAGCAATTGCTGGACGTGTAATGGCGAAGCGCGGTCCTTTCTTATTGATC
CAAGAAGTGTCTGGCAAGATCCAAGCTTACGCATCAAAAGATGTGCAAAAAGAGCTTAAAGAAAAATACCAAGGCCTAGA
TATCGGTGACATCATCGGTGTTAAAGGTGCTCTACATAAATCAGGTAAAGGCGATCTTTACGTGAATATGGAAGAGTTCG
TACTGCTAACTAAAGCACTACGTCCATTGCCTGAAAAATTCCACGGTTTAACTGACCAAGAAATGCGTTACCGTCAACGT
TATGTTGACCTTATCGTAAATGAAGATTCTCGTACTGCATTTATTATCCGCTCTAAAGTGGTTTCTGCAATTCGTAACTT
CATGGTTGATAAAGGCTTCATGGAAGTTGAAACGCCAATGATGCACGTTATCCCTGGTGGCGCATCGGCTCGTCCATTCG
TTACTCACCATAATGCACTTGATGTTGACATGTACTTACGTGTTGCACCTGAGCTTTATCTTAAACGTCTAGTTGTTGGT
GGTTTTGAGCGTGTATTCGAAATCAACCGTAACTTCCGTAATGAAGGTCTTTCTCCTCGTCATAATCCAGAATTCACAAT
GATGGAATTCTACATGGCGTACGCAGATTACAACGATCTTATGGATTTAACAGAAGCAATGCTATCTCAAGTGGCTATTT
CTGTTTTAGGCAGCGATAAAATGCCTTACGGCGAATACACAGTAGATTTTGGCGGTAAATATGCACGTTTGAGCATGTTA
GACGCAATCAAAATGTACAACCCTGAGCACGCTGAAATTCAAGCGCTAACGTATGACGGCGTTCAAGATCGTGATCTTAT
GGTTTCTATCGCGAAATCAGTTCATGTAGAAGTAGAAAGCTTCTGGACATGTGGTCAACTTCTTGAAGAAATCTTTGGTG
AAACAGCGGAACCTAAGCTAATGCAACCAACGTTCATTACTGAATACCCAGCGGATATTTCACCACTGGCTCGTCGTAAT
GATAATAATGCTTTCATTACTGACCGTTTTGAATTCTTCATTGGTGGTCGTGAAGTTGCAAATGGCTTCTCTGAGCTGAA
TGATGCACAAGATCAAGATGAACGCTTTAAAGCACAAGTAAGCGCGAAAGAATCTGGTGATGATGAAGCAATGTTCTACG
ATGCAGATTACATCACTGCACTTGAGCACGGCTTACCACCAACAGCAGGTCAAGGTATCGGTATTGACCGTCTAGTAATG
TTATTCACTAACACTCACACTATTCGTGACGTGATTTTATTCCCTTCTATGCGTCCACAAGCATAA

Upstream 100 bases:

>100_bases
ACCGGCATTGAAAATCGTAATACGCAAGCCGTTTTAGACGGCGATTTAGATAAATTTATCGAAGCCAGCTTAAAATCTGG
ATTATAAGAAAGGGTTCATT

Downstream 100 bases:

>100_bases
GTTGAATAAAAAAAATAGATAACAAATTGAAAGAGCGGCTTTTTAGTCGCTCTTTTTTTATCTGAATTATGACGATATAA
AGTGATAAATTTAAAATAAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTDQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAADLQSEFGEKTKEELEELNHIVAIAGRVMAKRGPFLLI
QEVSGKIQAYASKDVQKELKEKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEFVLLTKALRPLPEKFHGLTDQEMRYRQR
YVDLIVNEDSRTAFIIRSKVVSAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRVAPELYLKRLVVG
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEAMLSQVAISVLGSDKMPYGEYTVDFGGKYARLSML
DAIKMYNPEHAEIQALTYDGVQDRDLMVSIAKSVHVEVESFWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRN
DNNAFITDRFEFFIGGREVANGFSELNDAQDQDERFKAQVSAKESGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVM
LFTNTHTIRDVILFPSMRPQA

Sequences:

>Translated_501_residues
MTDQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAADLQSEFGEKTKEELEELNHIVAIAGRVMAKRGPFLLI
QEVSGKIQAYASKDVQKELKEKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEFVLLTKALRPLPEKFHGLTDQEMRYRQR
YVDLIVNEDSRTAFIIRSKVVSAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRVAPELYLKRLVVG
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEAMLSQVAISVLGSDKMPYGEYTVDFGGKYARLSML
DAIKMYNPEHAEIQALTYDGVQDRDLMVSIAKSVHVEVESFWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRN
DNNAFITDRFEFFIGGREVANGFSELNDAQDQDERFKAQVSAKESGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVM
LFTNTHTIRDVILFPSMRPQA
>Mature_500_residues
TDQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAADLQSEFGEKTKEELEELNHIVAIAGRVMAKRGPFLLIQ
EVSGKIQAYASKDVQKELKEKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEFVLLTKALRPLPEKFHGLTDQEMRYRQRY
VDLIVNEDSRTAFIIRSKVVSAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRVAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEAMLSQVAISVLGSDKMPYGEYTVDFGGKYARLSMLD
AIKMYNPEHAEIQALTYDGVQDRDLMVSIAKSVHVEVESFWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRND
NNAFITDRFEFFIGGREVANGFSELNDAQDQDERFKAQVSAKESGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPSMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=42.4849699398798, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=42.4849699398798, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI40789249, Length=244, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790571, Length=500, Percent_Identity=61.2, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=499, Percent_Identity=61.1222444889779, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI87082379, Length=331, Percent_Identity=30.5135951661631, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788173, Length=159, Percent_Identity=30.188679245283, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=506, Percent_Identity=38.9328063241107, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535925, Length=506, Percent_Identity=38.9328063241107, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=506, Percent_Identity=38.9328063241107, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32566633, Length=275, Percent_Identity=28.3636363636364, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320242, Length=520, Percent_Identity=39.4230769230769, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324256, Length=382, Percent_Identity=30.3664921465969, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323011, Length=361, Percent_Identity=26.0387811634349, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640849, Length=516, Percent_Identity=40.3100775193798, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640851, Length=516, Percent_Identity=40.3100775193798, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17136276, Length=464, Percent_Identity=23.0603448275862, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24584738, Length=292, Percent_Identity=27.7397260273973, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_ALISL (B6EMX2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262086.1
- GeneID:   6989004
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0566
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56874; Mature: 56743

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAADLQSEFGEKTKEELEEL
CCCCEECCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
NHIVAIAGRVMAKRGPFLLIQEVSGKIQAYASKDVQKELKEKYQGLDIGDIIGVKGALHK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
SGKGDLYVNMEEFVLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEDSRTAFIIRSKV
CCCCCEEECHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHEECCCCCEEEHHHHHH
VSAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRVAPELYLKRLVVG
HHHHHHHHHCCCCCEECCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEAMLSQVAISVLGSDK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
MPYGEYTVDFGGKYARLSMLDAIKMYNPEHAEIQALTYDGVQDRDLMVSIAKSVHVEVES
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHEEHHHH
FWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRNDNNAFITDRFEFFIGGREVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCEEEECCHHHH
NGFSELNDAQDQDERFKAQVSAKESGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVM
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHH
LFTNTHTIRDVILFPSMRPQA
HHHCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDQVQLDENKLIAERRGKLDHIRQACKANGHPNDFRRDSLAADLQSEFGEKTKEELEEL
CCCEECCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
NHIVAIAGRVMAKRGPFLLIQEVSGKIQAYASKDVQKELKEKYQGLDIGDIIGVKGALHK
HHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
SGKGDLYVNMEEFVLLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEDSRTAFIIRSKV
CCCCCEEECHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHEECCCCCEEEHHHHHH
VSAIRNFMVDKGFMEVETPMMHVIPGGASARPFVTHHNALDVDMYLRVAPELYLKRLVVG
HHHHHHHHHCCCCCEECCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GFERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYNDLMDLTEAMLSQVAISVLGSDK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
MPYGEYTVDFGGKYARLSMLDAIKMYNPEHAEIQALTYDGVQDRDLMVSIAKSVHVEVES
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHEEHHHH
FWTCGQLLEEIFGETAEPKLMQPTFITEYPADISPLARRNDNNAFITDRFEFFIGGREVA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCEEEECCHHHH
NGFSELNDAQDQDERFKAQVSAKESGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVM
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHH
LFTNTHTIRDVILFPSMRPQA
HHHCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA