Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 209694137

GI number: 209694137

Start: 597733

End: 598770

Strand: Direct

Name: pilT [H]

Synonym: VSAL_I0544

Alternate gene names: 209694137

Gene position: 597733-598770 (Clockwise)

Preceding gene: 209694129

Following gene: 209694138

Centisome position: 17.98

GC content: 40.85

Gene sequence:

>1038_bases
ATGGATATCACAGAACTACTGGACTTTAGTGTAAAACAAAACGCATCAGATCTACACCTATCTGCAGGAGTTCCTCCTAT
GGTACGTGTTGATGGTGAGGTTCGTAAACTCACATTACCTGATTTTACCCACGCTGATGTTCATCGCTTAGTGACTGATT
TCATGAATGATGCTCAACGAAGTGAGTTTGAAGAAAAGTTAGAAGTTGATTTTTCTTTTGAGCTTCCGAGTGTGGGGCGT
TTTCGTGTGAATGCGTTTAATCAATCACGAGGTTGTGCCGCTGTCTTTAGAACGATCCCTGTTGAGGTGCCTTCTCTTGA
TCAATTAGATGTCCCTGATATTTTTCTTGATATCGCAAAATATCAAAAAGGGTTGGTATTAATTACAGGGCCGACGGGAT
CGGGTAAATCAACAACATTAGCGGCGATGGTTGATTACGTTAATGAACATCATAATAAGCACATTCTCACGATCGAAGAT
CCGATTGAGTTTGTTCATAAAAATAAAAAATGTTTGATTAACCAACGTGAAGTTCATCGTGATACTCACAGTTTTAAAGC
GGCATTACGATCGGCATTGCGTGAGGATCCTGATGTCATTCTTGTGGGGGAATTGCGAGATCAAGAGACAATCAGTCTTG
CATTAACAGCAGCGGAAACCGGACACCTCGTGTTTGCTACGTTACACACCAGTTCTGCGGCAAAAACCATTGACCGTATT
ATTGATGTATTTCCTGGCAGTGATAAATCGATGGTGCGTTCAATGTTGTCGGAATCATTAAGAGCCGTGGTGGCTCAAAC
GTTATTAAAACGAATTGGTGGTGGACGAATTGCTTGTCATGAGATAATGATAGCAACCCCTGCAATACGTAACTTAATCC
GAGAAGATAAAGTCGCTCAGATGTATTCAATCATTCAGACTGGTTCCGCTTTTGGAATGCAAACCATGGAGCAAAATGCA
AAGAAAATTATTGCTCAAGGGTTAGTTGACCCTGATGAAGTTGCAAAGAAAGTTGAAATAACCAGTATGGGTTTTTAA

Upstream 100 bases:

>100_bases
GGGGATCGACCACATTTTTGAATGCTATTCTCTATCTGAAGAGTGATTTGGTGGATATTTTGCTTAATACTAGTCATAGA
AACGATTACCGAGGAAAATT

Downstream 100 bases:

>100_bases
TCATGAATTTGGATGATTTATTGAATCAGATGGTGAAGCAGAAAGCCTCTGATATTTATATTACGGTGGATGCACCGTGT
TTATTACGTGTTGATGGCGT

Product: twitching mobility protein PilT, type II/IV secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDFMNDAQRSEFEEKLEVDFSFELPSVGR
FRVNAFNQSRGCAAVFRTIPVEVPSLDQLDVPDIFLDIAKYQKGLVLITGPTGSGKSTTLAAMVDYVNEHHNKHILTIED
PIEFVHKNKKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSAFGMQTMEQNA
KKIIAQGLVDPDEVAKKVEITSMGF

Sequences:

>Translated_345_residues
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDFMNDAQRSEFEEKLEVDFSFELPSVGR
FRVNAFNQSRGCAAVFRTIPVEVPSLDQLDVPDIFLDIAKYQKGLVLITGPTGSGKSTTLAAMVDYVNEHHNKHILTIED
PIEFVHKNKKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSAFGMQTMEQNA
KKIIAQGLVDPDEVAKKVEITSMGF
>Mature_345_residues
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDFMNDAQRSEFEEKLEVDFSFELPSVGR
FRVNAFNQSRGCAAVFRTIPVEVPSLDQLDVPDIFLDIAKYQKGLVLITGPTGSGKSTTLAAMVDYVNEHHNKHILTIED
PIEFVHKNKKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQMYSIIQTGSAFGMQTMEQNA
KKIIAQGLVDPDEVAKKVEITSMGF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=46.7889908256881, Blast_Score=310, Evalue=6e-86,
Organism=Escherichia coli, GI1789723, Length=352, Percent_Identity=32.3863636363636, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1786296, Length=388, Percent_Identity=29.639175257732, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38233; Mature: 38233

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDFMNDAQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SEFEEKLEVDFSFELPSVGRFRVNAFNQSRGCAAVFRTIPVEVPSLDQLDVPDIFLDIAK
HHHHHHHCEEEEECCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
YQKGLVLITGPTGSGKSTTLAAMVDYVNEHHNKHILTIEDPIEFVHKNKKCLINQREVHR
HCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCHHCCCHHHHHH
DTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHH
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEECHHHHHHHHHHHHHH
MYSIIQTGSAFGMQTMEQNAKKIIAQGLVDPDEVAKKVEITSMGF
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHEEHHHCCCC
>Mature Secondary Structure
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHADVHRLVTDFMNDAQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
SEFEEKLEVDFSFELPSVGRFRVNAFNQSRGCAAVFRTIPVEVPSLDQLDVPDIFLDIAK
HHHHHHHCEEEEECCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
YQKGLVLITGPTGSGKSTTLAAMVDYVNEHHNKHILTIEDPIEFVHKNKKCLINQREVHR
HCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCHHCCCHHHHHH
DTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHH
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRIGGGRIACHEIMIATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHEECHHHHHHHHHHHHHH
MYSIIQTGSAFGMQTMEQNAKKIIAQGLVDPDEVAKKVEITSMGF
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]