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The map label for this gene is mpl [H]

Identifier: 209693951

GI number: 209693951

Start: 378499

End: 379860

Strand: Direct

Name: mpl [H]

Synonym: VSAL_I0347

Alternate gene names: 209693951

Gene position: 378499-379860 (Clockwise)

Preceding gene: 209693949

Following gene: 209693952

Centisome position: 11.38

GC content: 41.78

Gene sequence:

>1362_bases
ATGCATATTCATATCCTTGGCATCTGTGGCACCTTCATGGGAGGAGCCGCGACTTTAGCTCGTCAACTTGGACATAAGGT
TACAGGTAGTGATGCGAACGTTTACCCTCCAATGAGTACGTTATTAGAATCTCAGGGTATTGAGATCATTGAAGGCTTTG
ATCCTAGTCAGTTAGATCCTGCGCCAGACCTTGTTGTCATTGGGAATGCGATGAGTCGTGGTAACCCATGTGTGGAGTAT
GTTTTAGATAAAAATTTAAAATACACTTCAGGACCTCAATGGTTGCAAGAGTTCCTATTACAAGATCGCTGGGTACTGGC
TGTTGCAGGAACTCATGGTAAGACGACGACATCAAGCATGCTAGCCTGGGTCTTAGAAGAGTGTGGATATAAACCTGGTT
TTCTCGTCGGTGGTGTACTTGGTAACTTTGGGGAATCGGCACGATTAGGTGAAAGCATGTTTTTTGTGGTTGAAGCCGAT
GAATATGACAGTGCTTTTTTTGACAAGCGTTCTAAGTTTGTTCATTACCGTCCACGAACGTTAGTATTAAATAATCTGGA
ATTTGATCATGCTGATATTTTTGATGATCTTGCTGCAATTCAACGTCAGTTCCATCATCTTATAAGAACGGTGCCTAGTA
GTGGTCGTATTTTTGCCCCTAAACAAGATAAACCCTTAGCTGAAGTACTAGAGCAAGGATGTTGGAGTGAGATTGAATTT
ACGGGTGAAGAAGGAGAATGGAAAGCGAGTAAACTTAAGAAAGACGGCAGCCAATTTGACGTTTATTTCAATGATGAAAA
TGTTGGGCAGGTAAACTGGAATTTAGTCGGTGATCATAATGTAAGTAATGCTCTGATGGTCGTAGCTTCGGCTCGCCATG
TAGGCGTTACCCCAGATTTAGCTTGTGAAGCATTGGGCTCCTTTATCAATACTAAGCGTCGATTAGAGTTTAAAGGTGAG
GTTAATGGGGTCACGGTATATGACGATTTTGCTCATCACCCTACTGCAATTGATCTAACGTTAAGCGGTTTACGTAATAA
AGTGGGTGATAATAAAATTATCGCAGTATTAGAACCTCGCTCTAGTACGATGAAACTAGGGGTTCATAAAGACACCTTAG
CCGGTTCGCTTCATGCCGCAGATTCGGTGTATCTTTTCCAGCCTGATAATATTTCTTGGTCCGTTGATGATGTCGCTAAG
CAATGTGTTCAACCCGCCTATACTCATCATGAGATTAACGCTTTTGTTGATATGATTGTGGCTGAAGCAAAAGAAGGTGA
TCAAATTCTAGTCATGAGTAATGGTGGCTTTGAAGGCATTCACGGTAAATTACTATCAGCATTAGAGCAAAAGGCTATTT
AG

Upstream 100 bases:

>100_bases
GTTTACTGTAATTCATTCTATAGCACAGAACTTTTTAAGAGTATTGAAGTAACTCGATAACGGGATTACTCACTTAATTT
AATATAAGACGGTTTTTATT

Downstream 100 bases:

>100_bases
GATGTATAAAAAACAGAAAACAATCACCTTAGCAATGACTGGTGCCTCTGGTGCCCCTTATGGATTACGTCTATTGGAGT
GTTTGTTGGCTGCTGATTAT

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelat ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MHIHILGICGTFMGGAATLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLDPAPDLVVIGNAMSRGNPCVEY
VLDKNLKYTSGPQWLQEFLLQDRWVLAVAGTHGKTTTSSMLAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEAD
EYDSAFFDKRSKFVHYRPRTLVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSSGRIFAPKQDKPLAEVLEQGCWSEIEF
TGEEGEWKASKLKKDGSQFDVYFNDENVGQVNWNLVGDHNVSNALMVVASARHVGVTPDLACEALGSFINTKRRLEFKGE
VNGVTVYDDFAHHPTAIDLTLSGLRNKVGDNKIIAVLEPRSSTMKLGVHKDTLAGSLHAADSVYLFQPDNISWSVDDVAK
QCVQPAYTHHEINAFVDMIVAEAKEGDQILVMSNGGFEGIHGKLLSALEQKAI

Sequences:

>Translated_453_residues
MHIHILGICGTFMGGAATLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLDPAPDLVVIGNAMSRGNPCVEY
VLDKNLKYTSGPQWLQEFLLQDRWVLAVAGTHGKTTTSSMLAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEAD
EYDSAFFDKRSKFVHYRPRTLVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSSGRIFAPKQDKPLAEVLEQGCWSEIEF
TGEEGEWKASKLKKDGSQFDVYFNDENVGQVNWNLVGDHNVSNALMVVASARHVGVTPDLACEALGSFINTKRRLEFKGE
VNGVTVYDDFAHHPTAIDLTLSGLRNKVGDNKIIAVLEPRSSTMKLGVHKDTLAGSLHAADSVYLFQPDNISWSVDDVAK
QCVQPAYTHHEINAFVDMIVAEAKEGDQILVMSNGGFEGIHGKLLSALEQKAI
>Mature_453_residues
MHIHILGICGTFMGGAATLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLDPAPDLVVIGNAMSRGNPCVEY
VLDKNLKYTSGPQWLQEFLLQDRWVLAVAGTHGKTTTSSMLAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEAD
EYDSAFFDKRSKFVHYRPRTLVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSSGRIFAPKQDKPLAEVLEQGCWSEIEF
TGEEGEWKASKLKKDGSQFDVYFNDENVGQVNWNLVGDHNVSNALMVVASARHVGVTPDLACEALGSFINTKRRLEFKGE
VNGVTVYDDFAHHPTAIDLTLSGLRNKVGDNKIIAVLEPRSSTMKLGVHKDTLAGSLHAADSVYLFQPDNISWSVDDVAK
QCVQPAYTHHEINAFVDMIVAEAKEGDQILVMSNGGFEGIHGKLLSALEQKAI

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=453, Percent_Identity=67.991169977925, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=326, Percent_Identity=29.1411042944785, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49810; Mature: 49810

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGAATLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLDP
CEEEEEEEHHHHCCHHHHHHHHHCCEECCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
APDLVVIGNAMSRGNPCVEYVLDKNLKYTSGPQWLQEFLLQDRWVLAVAGTHGKTTTSSM
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEADEYDSAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSSGRIFAPKQDKPLAEVLEQGCWSEIEF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHCCCCCEEE
TGEEGEWKASKLKKDGSQFDVYFNDENVGQVNWNLVGDHNVSNALMVVASARHVGVTPDL
ECCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHH
ACEALGSFINTKRRLEFKGEVNGVTVYDDFAHHPTAIDLTLSGLRNKVGDNKIIAVLEPR
HHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCEEEEEHHHHHHCCCCCEEEEEECCC
SSTMKLGVHKDTLAGSLHAADSVYLFQPDNISWSVDDVAKQCVQPAYTHHEINAFVDMIV
CCEEEECCCHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
AEAKEGDQILVMSNGGFEGIHGKLLSALEQKAI
HHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGGAATLARQLGHKVTGSDANVYPPMSTLLESQGIEIIEGFDPSQLDP
CEEEEEEEHHHHCCHHHHHHHHHCCEECCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
APDLVVIGNAMSRGNPCVEYVLDKNLKYTSGPQWLQEFLLQDRWVLAVAGTHGKTTTSSM
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEADEYDSAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSSGRIFAPKQDKPLAEVLEQGCWSEIEF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHCCCCCEEE
TGEEGEWKASKLKKDGSQFDVYFNDENVGQVNWNLVGDHNVSNALMVVASARHVGVTPDL
ECCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHH
ACEALGSFINTKRRLEFKGEVNGVTVYDDFAHHPTAIDLTLSGLRNKVGDNKIIAVLEPR
HHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCEEEEEHHHHHHCCCCCEEEEEECCC
SSTMKLGVHKDTLAGSLHAADSVYLFQPDNISWSVDDVAKQCVQPAYTHHEINAFVDMIV
CCEEEECCCHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
AEAKEGDQILVMSNGGFEGIHGKLLSALEQKAI
HHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]