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The map label for this gene is rffA [H]

Identifier: 209693791

GI number: 209693791

Start: 214965

End: 216107

Strand: Reverse

Name: rffA [H]

Synonym: VSAL_I0174

Alternate gene names: 209693791

Gene position: 216107-214965 (Counterclockwise)

Preceding gene: 209693792

Following gene: 209693790

Centisome position: 6.5

GC content: 34.03

Gene sequence:

>1143_bases
ATGATTCCATTTAATAAACCACCAGTAACAGATAATGAGATTTCATATATTGATCAAGCAATTAAAAGTGGTTGTCTTGC
CGGAGATGGACATTTTTCAAAAAAATGTGAAACCTGGTTTGAAAATGCTACTGGAGCACAAAAAACCTTGTTAACACCTT
CATGTACTCACGCATTAGAGATGGCAGCTCTTCTTATTGACATTCAGCCTGGTGATGAAGTTATTATGCCAAGCTATACC
TTTGTTAGTACTGCAAATGCTTTTGTCTTAAGAGGTGCTAAAATTATTTTCGTTGATGTTCACCCTGAAACTATGAACAT
TAATGAGAATTTAATTGAAGCTGCAATAACAAAAAAAACAAAGGCGATTGTTCCTGTTCATTATGCTGGTGTTGCGTGTG
AAATGGATCTTATTATGAGTTTAGCTCATAAGTATTCATTATTCGTAATAGAGGATGCTGCTCAAGGGGTAATGTCTACT
TATAAAGGAAAAGCGTTAGGCTCTATTGGCCATTTAGGGGCTTTTAGTTTTCATGAAACTAAAAATTACACCAGTGGCGG
TGAGGGGGGACTATTGCTTATTAATGACTCTAGATTTATAAAACGAGCAGAGATACTAAGAGAAAAAGGAACAAATCGTA
GTCAATTCTTTAGGGGGATGGTAGATAAATACAGCTGGGTTGATGTTGGAAGCAGTTTCCTTCCTAGTGAAATTCAATCG
GCTTATTTATGGGCTCAATTAGGTAGTGCTGATAAAATTAATAAAAAAAGATTATCTGATTGGTTGTTTTACTTTAATAA
ACTACTTCCATTTCAGCATGAAGGTTTAATCGATTTACCTCATATACCGGAAGAAGTAAAACACAATGCTCACATGTTCT
ATATTAAAGTAGAAAATTTAGAGGTTAGAACTCAAGTTTTAAATAAACTTAAAGATCGAGGTATATTAGCTGTATTCCAC
TATATACCATTACATAGTTCTATCGCTGGTCTTCAATTTGGTCGTTTTCATGCTGAAGATAAATATACAACCGAAGAAAG
TGAACGATTGTTAAGATTGCCAATGTTTTACAACTTGTTAGAAAGTGAGAGAGAACTTGTGTGCAATGAGTTAATTAAGA
TTCTAAAGGAAATTCAATCATGA

Upstream 100 bases:

>100_bases
GCGTTTTTTTAACTGCATGATCATTAAGCCTGTTAGATAAGTTGAAAAATAATTTATGTTATAGTAAATAACATGTTTTA
CATAGCCTCAGAGTATTTTT

Downstream 100 bases:

>100_bases
TAGACATCAAAGATAAGCATGTTTTTTTTATCGCAGACAATGATCGATTTTTCTATTTATACGCAGATGCTTTAATTCAT
AGAGCTAAAATTTCTCCTGA

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIPFNKPPVTDNEISYIDQAIKSGCLAGDGHFSKKCETWFENATGAQKTLLTPSCTHALEMAALLIDIQPGDEVIMPSYT
FVSTANAFVLRGAKIIFVDVHPETMNINENLIEAAITKKTKAIVPVHYAGVACEMDLIMSLAHKYSLFVIEDAAQGVMST
YKGKALGSIGHLGAFSFHETKNYTSGGEGGLLLINDSRFIKRAEILREKGTNRSQFFRGMVDKYSWVDVGSSFLPSEIQS
AYLWAQLGSADKINKKRLSDWLFYFNKLLPFQHEGLIDLPHIPEEVKHNAHMFYIKVENLEVRTQVLNKLKDRGILAVFH
YIPLHSSIAGLQFGRFHAEDKYTTEESERLLRLPMFYNLLESERELVCNELIKILKEIQS

Sequences:

>Translated_380_residues
MIPFNKPPVTDNEISYIDQAIKSGCLAGDGHFSKKCETWFENATGAQKTLLTPSCTHALEMAALLIDIQPGDEVIMPSYT
FVSTANAFVLRGAKIIFVDVHPETMNINENLIEAAITKKTKAIVPVHYAGVACEMDLIMSLAHKYSLFVIEDAAQGVMST
YKGKALGSIGHLGAFSFHETKNYTSGGEGGLLLINDSRFIKRAEILREKGTNRSQFFRGMVDKYSWVDVGSSFLPSEIQS
AYLWAQLGSADKINKKRLSDWLFYFNKLLPFQHEGLIDLPHIPEEVKHNAHMFYIKVENLEVRTQVLNKLKDRGILAVFH
YIPLHSSIAGLQFGRFHAEDKYTTEESERLLRLPMFYNLLESERELVCNELIKILKEIQS
>Mature_380_residues
MIPFNKPPVTDNEISYIDQAIKSGCLAGDGHFSKKCETWFENATGAQKTLLTPSCTHALEMAALLIDIQPGDEVIMPSYT
FVSTANAFVLRGAKIIFVDVHPETMNINENLIEAAITKKTKAIVPVHYAGVACEMDLIMSLAHKYSLFVIEDAAQGVMST
YKGKALGSIGHLGAFSFHETKNYTSGGEGGLLLINDSRFIKRAEILREKGTNRSQFFRGMVDKYSWVDVGSSFLPSEIQS
AYLWAQLGSADKINKKRLSDWLFYFNKLLPFQHEGLIDLPHIPEEVKHNAHMFYIKVENLEVRTQVLNKLKDRGILAVFH
YIPLHSSIAGLQFGRFHAEDKYTTEESERLLRLPMFYNLLESERELVCNELIKILKEIQS

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=372, Percent_Identity=66.3978494623656, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI145693159, Length=382, Percent_Identity=30.8900523560209, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42856; Mature: 42856

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNKPPVTDNEISYIDQAIKSGCLAGDGHFSKKCETWFENATGAQKTLLTPSCTHALE
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCHHHHHHH
MAALLIDIQPGDEVIMPSYTFVSTANAFVLRGAKIIFVDVHPETMNINENLIEAAITKKT
HHHHEEEECCCCCEECCCCHHHHCCCEEEEECCEEEEEEECCCEECCCHHHHHHHHHHCC
KAIVPVHYAGVACEMDLIMSLAHKYSLFVIEDAAQGVMSTYKGKALGSIGHLGAFSFHET
CEEEEEEECCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCCHHCCCCCCCCCEECCC
KNYTSGGEGGLLLINDSRFIKRAEILREKGTNRSQFFRGMVDKYSWVDVGSSFLPSEIQS
CCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHH
AYLWAQLGSADKINKKRLSDWLFYFNKLLPFQHEGLIDLPHIPEEVKHNAHMFYIKVENL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHCCCCEEEEEEECCC
EVRTQVLNKLKDRGILAVFHYIPLHSSIAGLQFGRFHAEDKYTTEESERLLRLPMFYNLL
HHHHHHHHHHHHCCEEEEEEHHHHCCCHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHH
ESERELVCNELIKILKEIQS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPFNKPPVTDNEISYIDQAIKSGCLAGDGHFSKKCETWFENATGAQKTLLTPSCTHALE
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCHHHHHHH
MAALLIDIQPGDEVIMPSYTFVSTANAFVLRGAKIIFVDVHPETMNINENLIEAAITKKT
HHHHEEEECCCCCEECCCCHHHHCCCEEEEECCEEEEEEECCCEECCCHHHHHHHHHHCC
KAIVPVHYAGVACEMDLIMSLAHKYSLFVIEDAAQGVMSTYKGKALGSIGHLGAFSFHET
CEEEEEEECCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHCCCHHCCCCCCCCCEECCC
KNYTSGGEGGLLLINDSRFIKRAEILREKGTNRSQFFRGMVDKYSWVDVGSSFLPSEIQS
CCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCHHHHHH
AYLWAQLGSADKINKKRLSDWLFYFNKLLPFQHEGLIDLPHIPEEVKHNAHMFYIKVENL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHCCCCEEEEEEECCC
EVRTQVLNKLKDRGILAVFHYIPLHSSIAGLQFGRFHAEDKYTTEESERLLRLPMFYNLL
HHHHHHHHHHHHCCEEEEEEHHHHCCCHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHH
ESERELVCNELIKILKEIQS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]