Click here to switch to the map view.

The map label for this gene is fmt [H]

Identifier: 209693698

GI number: 209693698

Start: 77686

End: 78651

Strand: Reverse

Name: fmt [H]

Synonym: VSAL_I0074

Alternate gene names: 209693698

Gene position: 78651-77686 (Counterclockwise)

Preceding gene: 209693699

Following gene: 209693697

Centisome position: 2.37

GC content: 42.96

Gene sequence:

>966_bases
ATGATGACCGAGGTTAACTTGAACAAACCCTTACGCATTATATTTGCAGGTACGCCTGATTTCGCGGCTCGCCATTTAGC
CGCTCTACTTGATTCTCATCACGAGATTATTGGTGTTTATACACAGCCAGATCGCCCTGCGGGTCGAGGGAAGAAACTCA
CGGCAAGTCCTGTGAAAGAATTGGCACTAAAACATGCGATTCCAGTTTTCCAACCAGAGAACTTTAAAAGCGATGACGCG
AAACAAGAATTAGCTGACCAAAATGCAGATTTAATGGTTGTGGTTGCTTATGGTTTATTATTACCACAAGCAGTGCTTGA
TACACCAAAACTCGGTTGTATTAATGTTCACGGTTCTATTTTACCTCGCTGGCGTGGTGCCGCTCCTATCCAACGTTCAA
TTTGGGCTGGCGACGCAGAAACTGGCGTGACAATTATGCAAATGGACATCGGTTTAGATACTGGTGACATGCTAAATATC
ACCACACTGCCAATAGAAGCGACCGATACAAGCGCATCGATGTACAACAAACTTGCAGAGCTTGGTCCTATTGCTTTAGT
TAATTGCTTATCAGATATTGCAAACGGCAATGCCCTTGCAGAAAAACAAAATGATGAAAAAGCCAACTACGCAAAAAAAT
TAAGTAAAGAAGAAGCTAAAATCGATTGGACAATGGATGCAGAGACGATTGAACGTTGTGTTCGAGCGTTTAATCCTTGG
CCGATGAGTCACTTCTCTGTGGACGATAAAGCCATCAAAGTATGGCAGTCCCGTGTTGAAACCTACGAAGGTGATGCAAC
GCCAGGTACGATCATTCAGGCAGATAAAACGGGTATTTATGTAGCAACAAGCTCAAATGCGATCGTATTTGAACAACTGC
AAGTACCCGGAAAGAAAGCAATGGGCGTTCAAGATATTTTGAATTCTCGTAAAGAATGGTTCGAAGTGGGTAATACACTG
AACTAA

Upstream 100 bases:

>100_bases
TTGAAGGTAAATTATTCGTTGATTACTTGTCGCCATTAAAGCGCAAACGCATCAAAGAGAAGCTAGAAAAAATCAAAAAA
TTCAACGAAAAAAATTAGTC

Downstream 100 bases:

>100_bases
CTCTTCAAAACATAAAAAAGGTGGCATAATCGCCACCTTACTTTTTAAATGGCAAATATCGTATTTGCCATTTTATTTTG
CCAATCATATTTAGGTGATG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MMTEVNLNKPLRIIFAGTPDFAARHLAALLDSHHEIIGVYTQPDRPAGRGKKLTASPVKELALKHAIPVFQPENFKSDDA
KQELADQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLNI
TTLPIEATDTSASMYNKLAELGPIALVNCLSDIANGNALAEKQNDEKANYAKKLSKEEAKIDWTMDAETIERCVRAFNPW
PMSHFSVDDKAIKVWQSRVETYEGDATPGTIIQADKTGIYVATSSNAIVFEQLQVPGKKAMGVQDILNSRKEWFEVGNTL
N

Sequences:

>Translated_321_residues
MMTEVNLNKPLRIIFAGTPDFAARHLAALLDSHHEIIGVYTQPDRPAGRGKKLTASPVKELALKHAIPVFQPENFKSDDA
KQELADQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLNI
TTLPIEATDTSASMYNKLAELGPIALVNCLSDIANGNALAEKQNDEKANYAKKLSKEEAKIDWTMDAETIERCVRAFNPW
PMSHFSVDDKAIKVWQSRVETYEGDATPGTIIQADKTGIYVATSSNAIVFEQLQVPGKKAMGVQDILNSRKEWFEVGNTL
N
>Mature_321_residues
MMTEVNLNKPLRIIFAGTPDFAARHLAALLDSHHEIIGVYTQPDRPAGRGKKLTASPVKELALKHAIPVFQPENFKSDDA
KQELADQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLNI
TTLPIEATDTSASMYNKLAELGPIALVNCLSDIANGNALAEKQNDEKANYAKKLSKEEAKIDWTMDAETIERCVRAFNPW
PMSHFSVDDKAIKVWQSRVETYEGDATPGTIIQADKTGIYVATSSNAIVFEQLQVPGKKAMGVQDILNSRKEWFEVGNTL
N

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI21614513, Length=290, Percent_Identity=30, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI238814322, Length=259, Percent_Identity=29.7297297297297, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI164663775, Length=245, Percent_Identity=32.2448979591837, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=60.828025477707, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1788589, Length=261, Percent_Identity=29.1187739463602, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=313, Percent_Identity=24.6006389776358, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550868, Length=304, Percent_Identity=29.2763157894737, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=31.25, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585660, Length=239, Percent_Identity=33.4728033472803, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 35170; Mature: 35170

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTEVNLNKPLRIIFAGTPDFAARHLAALLDSHHEIIGVYTQPDRPAGRGKKLTASPVKE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHH
LALKHAIPVFQPENFKSDDAKQELADQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSI
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEEECCCCC
LPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLNITTLPIEATDTSASMYNKLAE
CCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHH
LGPIALVNCLSDIANGNALAEKQNDEKANYAKKLSKEEAKIDWTMDAETIERCVRAFNPW
HCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCC
PMSHFSVDDKAIKVWQSRVETYEGDATPGTIIQADKTGIYVATSSNAIVFEQLQVPGKKA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCEEEEEEECCCCHHC
MGVQDILNSRKEWFEVGNTLN
CCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMTEVNLNKPLRIIFAGTPDFAARHLAALLDSHHEIIGVYTQPDRPAGRGKKLTASPVKE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHH
LALKHAIPVFQPENFKSDDAKQELADQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSI
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEEECCCCC
LPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLNITTLPIEATDTSASMYNKLAE
CCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHH
LGPIALVNCLSDIANGNALAEKQNDEKANYAKKLSKEEAKIDWTMDAETIERCVRAFNPW
HCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHCCCC
PMSHFSVDDKAIKVWQSRVETYEGDATPGTIIQADKTGIYVATSSNAIVFEQLQVPGKKA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCEEEEEEECCCCHHC
MGVQDILNSRKEWFEVGNTLN
CCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA