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The map label for this gene is rsmB [H]

Identifier: 209693697

GI number: 209693697

Start: 76303

End: 77637

Strand: Reverse

Name: rsmB [H]

Synonym: VSAL_I0073

Alternate gene names: 209693697

Gene position: 77637-76303 (Counterclockwise)

Preceding gene: 209693698

Following gene: 209693696

Centisome position: 2.33

GC content: 43.0

Gene sequence:

>1335_bases
ATGGCAAATATCGTATTTGCCATTTTATTTTGCCAATCATATTTAGGTGATGACATGAACGTTCGTGCTGCGGCAGCCAA
AGTTATTTATCAAGTTGTCGATCAGGGTCATTCTCTCTCTTCAGCGCTTCCAAAGGCTCAATCTGAGATCAAGCCTCGTG
ACCAAGCCTTATTGCAAGAAATTTGCTACGGCGTACTGCGCTTTCTTCCTCGTTTAGAGTCGGTTGCTCAATCCTTAATG
GATAAACCATTAAAAGGAAAGCAACGTGTTTTCCATCATCTGATCCTAGTTGGTATTTATCAACTTAGTTTCATGCGTAT
TCCTGCACACGCTGCGGTAGGCGAAACCGTTGAAGGCACTAAAAACCTAAAAGGTCCAAAACTGCGTGGTTTGATCAACG
GTGTACTTCGAAGCTACCAACGTAGCCAAGAAGAATTAGATGCAAAATCAATCAGTCACGATTCAGGTAAATACGGTCAC
CCTGGTTGGTTGCTAAAAATGCTAAAAGAAGCGTACCCAACACAATGGGAATCGATTGTTGAAGCAAACAACAGCAAAGC
ACCTATGTGGTTACGAGTTAATAGCCAACACCACACTCGTGATGAATACATTGCATTACTTGAAAAAGAAGAGATTGCAG
TAACCCCTCACAGCCAAGCAACAGACGCTTTGTTATTAGATAAAGCGCGTGATGTATTTACATTGCCAGGCTTCGAAAAA
GGGTGGGTATCCGTACAAGATGCCGCTGCACAGCTATCTGTTGAGTATCTACAACCACAAGATGGTGAGCTGATTTTAGA
TTGCTGTGCCGCTCCAGGTGGTAAAACGGCGCACATTCTAGAGCGCACTAAAAACACCAACGTTGTCGCTATTGATTGTG
ATGAAACTCGCTTAACCCGTGTTTACGAAAATCTAGAACGTTTAAATCTTGAAGCTAAAGTGATTTGTGGCGATGCTCGT
CACCCTGAAGAATGGTGGCAAGGTGAACAATTTGATCGCATTTTATTAGATGCGCCTTGTTCTGCAACCGGAGTTATCCG
CCGCCACCCAGATATTAAATGGCTACGTCGTTCAGAAGACATCAAGGCACTTGCAGAATTACAAAGTGAAATCTTTGATG
CAATGTGGCTACAACTAAAATCAGGCGGCACCATGGTGTATGCAACTTGCTCAATCACACCACAAGAAAACGGTGATCAA
GTAAAAGCCTTTTTAGAACGTACTGAAAACGCGTCTCTGGTGGATAATACAGACCCAGCAAAACCTGGACGTCAAATTCT
TCCTGGTGAAGAAAATATGGATGGCTTCTATTACGCCGTTCTACAAAAAGCATAA

Upstream 100 bases:

>100_bases
TATTTTGAATTCTCGTAAAGAATGGTTCGAAGTGGGTAATACACTGAACTAACTCTTCAAAACATAAAAAAGGTGGCATA
ATCGCCACCTTACTTTTTAA

Downstream 100 bases:

>100_bases
TCGACTCACGAGCGAGCAATCGGTATGAAAATTATAATCTTAGGTGCAGGGCAGGTTGGCGGCACATTAGCTGAAAACTT
GGTGGGCGAAAACAACGATA

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MANIVFAILFCQSYLGDDMNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQSLM
DKPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVGETVEGTKNLKGPKLRGLINGVLRSYQRSQEELDAKSISHDSGKYGH
PGWLLKMLKEAYPTQWESIVEANNSKAPMWLRVNSQHHTRDEYIALLEKEEIAVTPHSQATDALLLDKARDVFTLPGFEK
GWVSVQDAAAQLSVEYLQPQDGELILDCCAAPGGKTAHILERTKNTNVVAIDCDETRLTRVYENLERLNLEAKVICGDAR
HPEEWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQLKSGGTMVYATCSITPQENGDQ
VKAFLERTENASLVDNTDPAKPGRQILPGEENMDGFYYAVLQKA

Sequences:

>Translated_444_residues
MANIVFAILFCQSYLGDDMNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQSLM
DKPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVGETVEGTKNLKGPKLRGLINGVLRSYQRSQEELDAKSISHDSGKYGH
PGWLLKMLKEAYPTQWESIVEANNSKAPMWLRVNSQHHTRDEYIALLEKEEIAVTPHSQATDALLLDKARDVFTLPGFEK
GWVSVQDAAAQLSVEYLQPQDGELILDCCAAPGGKTAHILERTKNTNVVAIDCDETRLTRVYENLERLNLEAKVICGDAR
HPEEWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQLKSGGTMVYATCSITPQENGDQ
VKAFLERTENASLVDNTDPAKPGRQILPGEENMDGFYYAVLQKA
>Mature_443_residues
ANIVFAILFCQSYLGDDMNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQSLMD
KPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVGETVEGTKNLKGPKLRGLINGVLRSYQRSQEELDAKSISHDSGKYGHP
GWLLKMLKEAYPTQWESIVEANNSKAPMWLRVNSQHHTRDEYIALLEKEEIAVTPHSQATDALLLDKARDVFTLPGFEKG
WVSVQDAAAQLSVEYLQPQDGELILDCCAAPGGKTAHILERTKNTNVVAIDCDETRLTRVYENLERLNLEAKVICGDARH
PEEWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQLKSGGTMVYATCSITPQENGDQV
KAFLERTENASLVDNTDPAKPGRQILPGEENMDGFYYAVLQKA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=344, Percent_Identity=32.5581395348837, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI76150623, Length=344, Percent_Identity=32.5581395348837, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI32698918, Length=186, Percent_Identity=33.8709677419355, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI301336155, Length=319, Percent_Identity=26.0188087774295, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270288818, Length=301, Percent_Identity=26.2458471760797, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI8922322, Length=301, Percent_Identity=26.2458471760797, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI23199998, Length=301, Percent_Identity=26.2458471760797, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI270288816, Length=301, Percent_Identity=26.2458471760797, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI40316918, Length=175, Percent_Identity=32, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI39995082, Length=215, Percent_Identity=30.2325581395349, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI11545785, Length=190, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI2367212, Length=424, Percent_Identity=58.0188679245283, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI87081985, Length=255, Percent_Identity=27.4509803921569, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=312, Percent_Identity=31.4102564102564, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25143471, Length=200, Percent_Identity=33.5, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71998419, Length=300, Percent_Identity=25.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=263, Percent_Identity=25.8555133079848, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=312, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319447, Length=199, Percent_Identity=32.1608040201005, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI22024126, Length=312, Percent_Identity=30.1282051282051, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19920494, Length=176, Percent_Identity=30.6818181818182, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356513, Length=235, Percent_Identity=30.6382978723404, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24668781, Length=235, Percent_Identity=30.6382978723404, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356579, Length=219, Percent_Identity=26.4840182648402, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49778; Mature: 49647

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIVFAILFCQSYLGDDMNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQE
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHH
ICYGVLRFLPRLESVAQSLMDKPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVGETVEGT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
KNLKGPKLRGLINGVLRSYQRSQEELDAKSISHDSGKYGHPGWLLKMLKEAYPTQWESIV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
EANNSKAPMWLRVNSQHHTRDEYIALLEKEEIAVTPHSQATDALLLDKARDVFTLPGFEK
HCCCCCCCEEEEECCCCCCHHHHHHHEECCCEEECCCCCCHHHHHHHCCCCEEECCCCCC
GWVSVQDAAAQLSVEYLQPQDGELILDCCAAPGGKTAHILERTKNTNVVAIDCDETRLTR
CCEEHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEECCHHHHHH
VYENLERLNLEAKVICGDARHPEEWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRSED
HHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHCCHH
IKALAELQSEIFDAMWLQLKSGGTMVYATCSITPQENGDQVKAFLERTENASLVDNTDPA
HHHHHHHHHHHHHHHHHHEECCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCC
KPGRQILPGEENMDGFYYAVLQKA
CCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
ANIVFAILFCQSYLGDDMNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHH
ICYGVLRFLPRLESVAQSLMDKPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVGETVEGT
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
KNLKGPKLRGLINGVLRSYQRSQEELDAKSISHDSGKYGHPGWLLKMLKEAYPTQWESIV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHH
EANNSKAPMWLRVNSQHHTRDEYIALLEKEEIAVTPHSQATDALLLDKARDVFTLPGFEK
HCCCCCCCEEEEECCCCCCHHHHHHHEECCCEEECCCCCCHHHHHHHCCCCEEECCCCCC
GWVSVQDAAAQLSVEYLQPQDGELILDCCAAPGGKTAHILERTKNTNVVAIDCDETRLTR
CCEEHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEECCHHHHHH
VYENLERLNLEAKVICGDARHPEEWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRSED
HHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHCCHH
IKALAELQSEIFDAMWLQLKSGGTMVYATCSITPQENGDQVKAFLERTENASLVDNTDPA
HHHHHHHHHHHHHHHHHHEECCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCC
KPGRQILPGEENMDGFYYAVLQKA
CCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]