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The map label for this gene is tgt-1 [H]

Identifier: 197119699

GI number: 197119699

Start: 3831068

End: 3832174

Strand: Direct

Name: tgt-1 [H]

Synonym: Gbem_3335

Alternate gene names: 197119699

Gene position: 3831068-3832174 (Clockwise)

Preceding gene: 197119690

Following gene: 197119700

Centisome position: 83.01

GC content: 61.97

Gene sequence:

>1107_bases
ATGATTTTCAAACTGCTTAAGAAAGATCCCAAGTCGGGAGCGCGCCGCGGCGTGGTGACCACCGCCCACGGAGAGATCCA
GACCCCGATTTTCATGCCTGTTGCGACCCACGCCGCCATGAAGGCCATGACCCCCGCGCAGGTGCACGACACCGGCGCGC
AGATCATCCTCTCCAACACCTACCATCTGCACCTGCACCCCGGCGAGGCCCTGGTGCAAAAAGCAGGCGGGCTGCACCAG
TTCATGGGGTGGGACGGCCCCATCCTCACCGACTCCGGCGGATTCCAGGTCTTCTCGCTCCCCAAGAAGCGGATCACCGA
GGAGGGGGTTTTCTTCCGCCACGAGGACACCGGCGAAGAGGTCTTCCTCGACCCGGCCAAGGCGGTCGCCATCCAGGAGG
CCTTGGGCGCCGACATCATCATGAACTTCGACGAGTGCATCCCGTATCCCTGCGAGAAATCCTACGCCGAGCGCTCCACC
GAGAAGACCATCCGCTGGGCCAAGCAGTGCCGGGAGGCGCACAAAAGGGAGAAGCAGTATCTCTTCGGCATCGTGCAGGG
GAGCGTGTTCGAGGACCTGAGGAAGCGCTGCGCCAAGGAGCTGGTGAAGATGGACTTCCCCGGCTACGCCATCGGCGGCG
TTTCGGTAGGCGAGGGGCTTGAGCTTTTGAAGCAGGTGGTGGGGTACACCGCACCGCACCTTCCCGAGGACAAGCCGCGC
TACCTGATGGGGGTCGGCCTTCCCGAGGACATACTGGAGAGCGTCGAGCGCGGCATCGACATGTTCGACTGCGTGATACC
GACCCGCTACGCCAGGAGCGCCACGCTCTTCACCAATCGCGGCCGTATCCGGCTCACCAACAAGAACTACCGGCGCGACT
TCTACCCTATCGAGCCCAACTGCACCTGCTACACCTGCCGCAACTTCACCCGCGCCTACCTGCACCACCTCTTCACGGCG
AACGAGGTGCTCTCGGCGATACTGGCAAGCATCCACAACGTGCATTTCTACCTGAACATGATGGCCGAGATCCGCAACTC
CATCGAGGAGGGGCGCTTCAAGGAGTACAAAGAAACGTTCCTGGCGTCCTATCTCAAGGGGAAATAG

Upstream 100 bases:

>100_bases
AAATTCCGCTTACGCCCCCTTGCTGTTGCTTTTTCCCACCCGCTCTGCTAATTAGGATAGTTACCGCAAGGAAAAATTCA
GAAAATAGAACGGAAGGTCG

Downstream 100 bases:

>100_bases
CCGTTATGAACCGAAACCTGCACCTGATACTGCTCCTGCTTCTCGCCCTTCTTGCCTCCTCCTGCTCCAAGGGGAAACAA
CAACCCCCTGAAGGGAGAAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIFKLLKKDPKSGARRGVVTTAHGEIQTPIFMPVATHAAMKAMTPAQVHDTGAQIILSNTYHLHLHPGEALVQKAGGLHQ
FMGWDGPILTDSGGFQVFSLPKKRITEEGVFFRHEDTGEEVFLDPAKAVAIQEALGADIIMNFDECIPYPCEKSYAERST
EKTIRWAKQCREAHKREKQYLFGIVQGSVFEDLRKRCAKELVKMDFPGYAIGGVSVGEGLELLKQVVGYTAPHLPEDKPR
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTNRGRIRLTNKNYRRDFYPIEPNCTCYTCRNFTRAYLHHLFTA
NEVLSAILASIHNVHFYLNMMAEIRNSIEEGRFKEYKETFLASYLKGK

Sequences:

>Translated_368_residues
MIFKLLKKDPKSGARRGVVTTAHGEIQTPIFMPVATHAAMKAMTPAQVHDTGAQIILSNTYHLHLHPGEALVQKAGGLHQ
FMGWDGPILTDSGGFQVFSLPKKRITEEGVFFRHEDTGEEVFLDPAKAVAIQEALGADIIMNFDECIPYPCEKSYAERST
EKTIRWAKQCREAHKREKQYLFGIVQGSVFEDLRKRCAKELVKMDFPGYAIGGVSVGEGLELLKQVVGYTAPHLPEDKPR
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTNRGRIRLTNKNYRRDFYPIEPNCTCYTCRNFTRAYLHHLFTA
NEVLSAILASIHNVHFYLNMMAEIRNSIEEGRFKEYKETFLASYLKGK
>Mature_368_residues
MIFKLLKKDPKSGARRGVVTTAHGEIQTPIFMPVATHAAMKAMTPAQVHDTGAQIILSNTYHLHLHPGEALVQKAGGLHQ
FMGWDGPILTDSGGFQVFSLPKKRITEEGVFFRHEDTGEEVFLDPAKAVAIQEALGADIIMNFDECIPYPCEKSYAERST
EKTIRWAKQCREAHKREKQYLFGIVQGSVFEDLRKRCAKELVKMDFPGYAIGGVSVGEGLELLKQVVGYTAPHLPEDKPR
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTNRGRIRLTNKNYRRDFYPIEPNCTCYTCRNFTRAYLHHLFTA
NEVLSAILASIHNVHFYLNMMAEIRNSIEEGRFKEYKETFLASYLKGK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=44.2307692307692, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI13375872, Length=359, Percent_Identity=25.6267409470752, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1786607, Length=363, Percent_Identity=52.0661157024793, Blast_Score=395, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17544680, Length=353, Percent_Identity=42.7762039660057, Blast_Score=296, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI25146502, Length=258, Percent_Identity=26.7441860465116, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920510, Length=370, Percent_Identity=42.972972972973, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21355311, Length=167, Percent_Identity=33.5329341317365, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41704; Mature: 41704

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKLLKKDPKSGARRGVVTTAHGEIQTPIFMPVATHAAMKAMTPAQVHDTGAQIILSNT
CCCHHHHCCCCCHHHCCCEEECCCCCCCCEEEHHHHHHHHHHCCCHHHHCCCCEEEEECE
YHLHLHPGEALVQKAGGLHQFMGWDGPILTDSGGFQVFSLPKKRITEEGVFFRHEDTGEE
EEEEECCHHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHCCCCCEEEECCCCCE
VFLDPAKAVAIQEALGADIIMNFDECIPYPCEKSYAERSTEKTIRWAKQCREAHKREKQY
EEECHHHHHHHHHHHCCHHEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGIVQGSVFEDLRKRCAKELVKMDFPGYAIGGVSVGEGLELLKQVVGYTAPHLPEDKPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTNRGRIRLTNKNYRRDFYPIEPN
EEEECCCCHHHHHHHHHCCHHHHHHCCHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCC
CTCYTCRNFTRAYLHHLFTANEVLSAILASIHNVHFYLNMMAEIRNSIEEGRFKEYKETF
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LASYLKGK
HHHHHCCC
>Mature Secondary Structure
MIFKLLKKDPKSGARRGVVTTAHGEIQTPIFMPVATHAAMKAMTPAQVHDTGAQIILSNT
CCCHHHHCCCCCHHHCCCEEECCCCCCCCEEEHHHHHHHHHHCCCHHHHCCCCEEEEECE
YHLHLHPGEALVQKAGGLHQFMGWDGPILTDSGGFQVFSLPKKRITEEGVFFRHEDTGEE
EEEEECCHHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHCCCCCEEEECCCCCE
VFLDPAKAVAIQEALGADIIMNFDECIPYPCEKSYAERSTEKTIRWAKQCREAHKREKQY
EEECHHHHHHHHHHHCCHHEECHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGIVQGSVFEDLRKRCAKELVKMDFPGYAIGGVSVGEGLELLKQVVGYTAPHLPEDKPR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
YLMGVGLPEDILESVERGIDMFDCVIPTRYARSATLFTNRGRIRLTNKNYRRDFYPIEPN
EEEECCCCHHHHHHHHHCCHHHHHHCCHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCC
CTCYTCRNFTRAYLHHLFTANEVLSAILASIHNVHFYLNMMAEIRNSIEEGRFKEYKETF
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LASYLKGK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA