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The map label for this gene is ruvA

Identifier: 197119680

GI number: 197119680

Start: 3811654

End: 3812253

Strand: Direct

Name: ruvA

Synonym: Gbem_3315

Alternate gene names: 197119680

Gene position: 3811654-3812253 (Clockwise)

Preceding gene: 197119679

Following gene: 197119681

Centisome position: 82.59

GC content: 60.83

Gene sequence:

>600_bases
ATGATCGCGCTCCTTACCGGAAAGCTCGCCTACAAATCCCCCGAGTTCATCATCCTGGACGTCAACGGCGTGGGATACCA
GGTGCACATCCCCTTCTCCACCTACTACACCCTCCCCGTCGAAGGGGGCGCGCTGTCGCTGCAGATCCACACCTCGGTGA
AGGAAGACGCCATCAACCTGTACGGCTTCCGAACCCAGCAGGAGAAGGAACTGTTCCAGCTCCTGATCGGCGTCTCAGGC
GTGGGGCCGAAGCTCGCCACCGGCATACTTTCCAACAGCGAGCCTTCCGAGCTTGCCGATTCGCTCGTCAACGGCAACCT
GGCCCGGCTCTCCGCCATTCCCGGCATCGGCAAGAAAACAGCGGAGCGCCTGGTGCTGGAACTTAAGGAAAAGATGAAGA
AGCTGGGCCTCGCGCAGCCGCAGGCAGGCGGCACCACCGCTCCGGCCAAACAGGAGATCAGGGACGACGTGCTCTCCGCG
CTCATCAACCTGGGGTACAAGGAAGCCGTGGTGCAAAAGGCACTGGCAGAGTTGAAGGTCACGGAAGACGCGACCGTCGA
ACTCGTGCTCAAGCAGGCGCTGAAGATACTGATGAAGTAG

Upstream 100 bases:

>100_bases
CAGGCATCAATGCCCTTTTTAAAAACGTCAGGTAGCCACTAATGTTTCTTCACCGTCAATTGTCAATTGTCCATTGCCAA
TTGGTTCAGGAGAGTTTTTT

Downstream 100 bases:

>100_bases
GCGACGCAGCAGGCGTATCCCTCCGGCATGCCCCTTGACCTTTATCTCCAACTGTTATAATTTGGCCCCTTGCATTTTGC
AGGATTGCCGCAGCAAGGAG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIALLTGKLAYKSPEFIILDVNGVGYQVHIPFSTYYTLPVEGGALSLQIHTSVKEDAINLYGFRTQQEKELFQLLIGVSG
VGPKLATGILSNSEPSELADSLVNGNLARLSAIPGIGKKTAERLVLELKEKMKKLGLAQPQAGGTTAPAKQEIRDDVLSA
LINLGYKEAVVQKALAELKVTEDATVELVLKQALKILMK

Sequences:

>Translated_199_residues
MIALLTGKLAYKSPEFIILDVNGVGYQVHIPFSTYYTLPVEGGALSLQIHTSVKEDAINLYGFRTQQEKELFQLLIGVSG
VGPKLATGILSNSEPSELADSLVNGNLARLSAIPGIGKKTAERLVLELKEKMKKLGLAQPQAGGTTAPAKQEIRDDVLSA
LINLGYKEAVVQKALAELKVTEDATVELVLKQALKILMK
>Mature_199_residues
MIALLTGKLAYKSPEFIILDVNGVGYQVHIPFSTYYTLPVEGGALSLQIHTSVKEDAINLYGFRTQQEKELFQLLIGVSG
VGPKLATGILSNSEPSELADSLVNGNLARLSAIPGIGKKTAERLVLELKEKMKKLGLAQPQAGGTTAPAKQEIRDDVLSA
LINLGYKEAVVQKALAELKVTEDATVELVLKQALKILMK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=201, Percent_Identity=37.3134328358209, Blast_Score=134, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_GEOBB (B5EAH2)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002140107.1
- ProteinModelPortal:   B5EAH2
- SMR:   B5EAH2
- GeneID:   6780173
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3315
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21412; Mature: 21412

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALLTGKLAYKSPEFIILDVNGVGYQVHIPFSTYYTLPVEGGALSLQIHTSVKEDAINL
CEEEEECCHHCCCCCEEEEEECCCEEEEEECCEEEEEEEECCCEEEEEEECCCCHHHHHH
YGFRTQQEKELFQLLIGVSGVGPKLATGILSNSEPSELADSLVNGNLARLSAIPGIGKKT
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHH
AERLVLELKEKMKKLGLAQPQAGGTTAPAKQEIRDDVLSALINLGYKEAVVQKALAELKV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
TEDATVELVLKQALKILMK
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIALLTGKLAYKSPEFIILDVNGVGYQVHIPFSTYYTLPVEGGALSLQIHTSVKEDAINL
CEEEEECCHHCCCCCEEEEEECCCEEEEEECCEEEEEEEECCCEEEEEEECCCCHHHHHH
YGFRTQQEKELFQLLIGVSGVGPKLATGILSNSEPSELADSLVNGNLARLSAIPGIGKKT
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHH
AERLVLELKEKMKKLGLAQPQAGGTTAPAKQEIRDDVLSALINLGYKEAVVQKALAELKV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
TEDATVELVLKQALKILMK
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA