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The map label for this gene is zraS [H]

Identifier: 197118191

GI number: 197118191

Start: 2089667

End: 2091739

Strand: Direct

Name: zraS [H]

Synonym: Gbem_1806

Alternate gene names: 197118191

Gene position: 2089667-2091739 (Clockwise)

Preceding gene: 197118190

Following gene: 197118192

Centisome position: 45.28

GC content: 62.9

Gene sequence:

>2073_bases
ATGGCGCTTTCGCTTGTTGCGATCATCTTGCTTTTGTTTGCCATGCTGAGGATAATCCGGCTGGAGCGCAAAAGCGCCTG
CCGCACCTTATTGGCCATCCCCTTTTGCGCCGTCGCCTTCCTGGAGTTCTTCGACTTTGCGGCGCTGTACCAGTTCTTCC
CCGAGGTGGACTGGAAGCGGTTGTCGCTTGGGGTCGAGGCGCTGCTCCCGGCGCTTTGGCTGCTGGTGAGCCTCACCTAT
GCCCGGGACCTGCCCGAACGCGGGCTTTCGCGCTCGACCCGCCTGATGCTCGCCGGCGCCTTCGCTTTGGTCCTGGTCCC
CGTCTTGATGCCGGGGGAGGCTCTTTACTACGCTCCCGACTTCCCTCTGGAGCGGATGCTTTTTCTCACCGACGTCGGGT
ACTACTTCTACGTCGCGATGCTGGTCCTTTTGGTGGTGGCGCTCATGAACCTCGAGTCCACCCTGGTGAACGCCTCCTCC
GAGGCGCTTTGGCGCGTTAAGCTCGACATCGTGGCGCTGGGCTCCGTGCTCGCCGTCTGCGTCTTCTACTACACCAACGC
GCTCTTGTACCGCTCGCTCAACATGGAGCTGGTTCCCCTGCGCTCGCTTGTTTCCATCATCGCCGCGCTGATGATCGTCT
ACTCGCGCACCCACTGGAGGGGAACGGCGCAGGTGAAGGTCTCGCAGGCGGTCCTTTTGAAGTCCGTTGTCCTCACCGTA
ATCGCCGGGTATCTGTTGCTCCTGGGGTCCATCGGTGAGGGGATGAAGTACTTCGGCGCGCTCTTTCCCCGCGTGCTCGC
CCTGTCGCTTGGGTTCATCGCGGGGATGCTCCTGCTCCTTTTGCTCCTCTCCGAGCGCGCCCAAAGGGAGCTGAAGGTCT
TCCTCCATAAGCACTTCTACCAGAGCAAGTACGACTACCGGGCCCAGTGGCTGGGGCTCACCGAACGCCTGGCCAATTTC
GAGAGCGGCGACGGGCTTTTGAGGCTGGTCCTCTCCGCCTACTGCGACATCTTCGGAGTGAGGGGGGGGGCGCTGTTCCT
GCACCAGGACGGGTGCGGCTGGTTCTGCGCCACTGGGATCCAGGAGCTGGAAGAGGTCAGGGTAACCATCGCCGCCGACA
ACCCGCTCATCGCCTATCTGCGCGACCGCCGCTGGGTCTTTTGCAGCCGGGACGACAACCCGGAGATCCTGGAGGCCAAC
TGCCGCTGGCTTAGGGAACTGAAGGTGAGCTTCGCCATCCCTCTTTTCGAGGGGGAGGCGCTAACCGGTTTCATCGTTTT
GGGCGAGCAGGTGGTTCCGGACGAAGAGTACCGCTACGAGGATTACGACCTGATGAAGGCGATCGCGCGCCAGGCCTCGG
TGGCGATACAGCACCAGCGCCTCTCCGAGCAACTCACCCAGGCGAAAGCGATGGAGGCGGTGGGGAATCTGGCCACTTTC
GTGGTGCACGACCTGAAAAACCTGGCGGCCACCGTATCGCTCGTGGTGGAAAACGCCCGGGAGCACCTGGACAACCCCGA
GTTCCAAAAGGACATGCTCTCAAGCCTCGGCAACACCACGAGGAAGATGCACACGCTCATAGGCCGCCTGCGAAACCTCG
GCGAGAGCGAACTCTTGCACATGCGCCCCGTAGACCTGCTGGCGTTGGCCCGGCACTCGGCGCGCCAGGTGCAGGGGAGG
GCGGTCACGGTGCAGGGGACGCCGGAGAAGGTCATAGGCGACGAGGAAGAATTGGAGAAGGTGCTTTTGAACCTCTTCCT
GAACGCGGTGGAGGCTTCCGAGCCGGGGACCCAGATCGTGGCGGAGGTGGGATGCGCGGACTCCCCCTACCTCAAGGTGT
CGGACAGCGGCTGCGGCATGTCGCCGCTTTTCATACGGAACGAACTTTTTGCACCGTTCAAGACCACGAAGCAGGCGGGG
CTCGGCATCGGGCTCTACCAGTGCCGGCAGATCGTGGCGGCGCACGGCGGCAGGATCGAGGTATGCAGCGTAGAGGGGGA
GGGGACCGTGTTCACGGTATGGTTCCCGTCCCCTGCGCTAGGCGCGGAACGGGTCATCACACAGCCGGCATGA

Upstream 100 bases:

>100_bases
CGGTCTTGATCGACGTGAAGAGCATGTTCGACCGGGAGCGGGTTGAAGAGGCCGGTGTCAAGGTCTGGGCGTTATAAAGG
GTTGAATCCCAGAGGGGAAC

Downstream 100 bases:

>100_bases
GGGGGTGGTAGTGAAAAAACTATTGATTGTCGACGACAACGACGATATCAGGCAGCAGTTGAAGTGGGGCCTGAACCAGG
AATTCCAGGTGCTTTTGGCG

Product: GAF domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MALSLVAIILLLFAMLRIIRLERKSACRTLLAIPFCAVAFLEFFDFAALYQFFPEVDWKRLSLGVEALLPALWLLVSLTY
ARDLPERGLSRSTRLMLAGAFALVLVPVLMPGEALYYAPDFPLERMLFLTDVGYYFYVAMLVLLVVALMNLESTLVNASS
EALWRVKLDIVALGSVLAVCVFYYTNALLYRSLNMELVPLRSLVSIIAALMIVYSRTHWRGTAQVKVSQAVLLKSVVLTV
IAGYLLLLGSIGEGMKYFGALFPRVLALSLGFIAGMLLLLLLLSERAQRELKVFLHKHFYQSKYDYRAQWLGLTERLANF
ESGDGLLRLVLSAYCDIFGVRGGALFLHQDGCGWFCATGIQELEEVRVTIAADNPLIAYLRDRRWVFCSRDDNPEILEAN
CRWLRELKVSFAIPLFEGEALTGFIVLGEQVVPDEEYRYEDYDLMKAIARQASVAIQHQRLSEQLTQAKAMEAVGNLATF
VVHDLKNLAATVSLVVENAREHLDNPEFQKDMLSSLGNTTRKMHTLIGRLRNLGESELLHMRPVDLLALARHSARQVQGR
AVTVQGTPEKVIGDEEELEKVLLNLFLNAVEASEPGTQIVAEVGCADSPYLKVSDSGCGMSPLFIRNELFAPFKTTKQAG
LGIGLYQCRQIVAAHGGRIEVCSVEGEGTVFTVWFPSPALGAERVITQPA

Sequences:

>Translated_690_residues
MALSLVAIILLLFAMLRIIRLERKSACRTLLAIPFCAVAFLEFFDFAALYQFFPEVDWKRLSLGVEALLPALWLLVSLTY
ARDLPERGLSRSTRLMLAGAFALVLVPVLMPGEALYYAPDFPLERMLFLTDVGYYFYVAMLVLLVVALMNLESTLVNASS
EALWRVKLDIVALGSVLAVCVFYYTNALLYRSLNMELVPLRSLVSIIAALMIVYSRTHWRGTAQVKVSQAVLLKSVVLTV
IAGYLLLLGSIGEGMKYFGALFPRVLALSLGFIAGMLLLLLLLSERAQRELKVFLHKHFYQSKYDYRAQWLGLTERLANF
ESGDGLLRLVLSAYCDIFGVRGGALFLHQDGCGWFCATGIQELEEVRVTIAADNPLIAYLRDRRWVFCSRDDNPEILEAN
CRWLRELKVSFAIPLFEGEALTGFIVLGEQVVPDEEYRYEDYDLMKAIARQASVAIQHQRLSEQLTQAKAMEAVGNLATF
VVHDLKNLAATVSLVVENAREHLDNPEFQKDMLSSLGNTTRKMHTLIGRLRNLGESELLHMRPVDLLALARHSARQVQGR
AVTVQGTPEKVIGDEEELEKVLLNLFLNAVEASEPGTQIVAEVGCADSPYLKVSDSGCGMSPLFIRNELFAPFKTTKQAG
LGIGLYQCRQIVAAHGGRIEVCSVEGEGTVFTVWFPSPALGAERVITQPA
>Mature_689_residues
ALSLVAIILLLFAMLRIIRLERKSACRTLLAIPFCAVAFLEFFDFAALYQFFPEVDWKRLSLGVEALLPALWLLVSLTYA
RDLPERGLSRSTRLMLAGAFALVLVPVLMPGEALYYAPDFPLERMLFLTDVGYYFYVAMLVLLVVALMNLESTLVNASSE
ALWRVKLDIVALGSVLAVCVFYYTNALLYRSLNMELVPLRSLVSIIAALMIVYSRTHWRGTAQVKVSQAVLLKSVVLTVI
AGYLLLLGSIGEGMKYFGALFPRVLALSLGFIAGMLLLLLLLSERAQRELKVFLHKHFYQSKYDYRAQWLGLTERLANFE
SGDGLLRLVLSAYCDIFGVRGGALFLHQDGCGWFCATGIQELEEVRVTIAADNPLIAYLRDRRWVFCSRDDNPEILEANC
RWLRELKVSFAIPLFEGEALTGFIVLGEQVVPDEEYRYEDYDLMKAIARQASVAIQHQRLSEQLTQAKAMEAVGNLATFV
VHDLKNLAATVSLVVENAREHLDNPEFQKDMLSSLGNTTRKMHTLIGRLRNLGESELLHMRPVDLLALARHSARQVQGRA
VTVQGTPEKVIGDEEELEKVLLNLFLNAVEASEPGTQIVAEVGCADSPYLKVSDSGCGMSPLFIRNELFAPFKTTKQAGL
GIGLYQCRQIVAAHGGRIEVCSVEGEGTVFTVWFPSPALGAERVITQPA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=29.5652173913043, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=28.0172413793103, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76822; Mature: 76691

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSLVAIILLLFAMLRIIRLERKSACRTLLAIPFCAVAFLEFFDFAALYQFFPEVDWKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSLGVEALLPALWLLVSLTYARDLPERGLSRSTRLMLAGAFALVLVPVLMPGEALYYAPD
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
FPLERMLFLTDVGYYFYVAMLVLLVVALMNLESTLVNASSEALWRVKLDIVALGSVLAVC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
VFYYTNALLYRSLNMELVPLRSLVSIIAALMIVYSRTHWRGTAQVKVSQAVLLKSVVLTV
HHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
IAGYLLLLGSIGEGMKYFGALFPRVLALSLGFIAGMLLLLLLLSERAQRELKVFLHKHFY
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSKYDYRAQWLGLTERLANFESGDGLLRLVLSAYCDIFGVRGGALFLHQDGCGWFCATGI
HCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
QELEEVRVTIAADNPLIAYLRDRRWVFCSRDDNPEILEANCRWLRELKVSFAIPLFEGEA
HHHHHCEEEEECCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCC
LTGFIVLGEQVVPDEEYRYEDYDLMKAIARQASVAIQHQRLSEQLTQAKAMEAVGNLATF
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVHDLKNLAATVSLVVENAREHLDNPEFQKDMLSSLGNTTRKMHTLIGRLRNLGESELLH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHEEC
MRPVDLLALARHSARQVQGRAVTVQGTPEKVIGDEEELEKVLLNLFLNAVEASEPGTQIV
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
AEVGCADSPYLKVSDSGCGMSPLFIRNELFAPFKTTKQAGLGIGLYQCRQIVAAHGGRIE
HHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCEEE
VCSVEGEGTVFTVWFPSPALGAERVITQPA
EEEECCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALSLVAIILLLFAMLRIIRLERKSACRTLLAIPFCAVAFLEFFDFAALYQFFPEVDWKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LSLGVEALLPALWLLVSLTYARDLPERGLSRSTRLMLAGAFALVLVPVLMPGEALYYAPD
HHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
FPLERMLFLTDVGYYFYVAMLVLLVVALMNLESTLVNASSEALWRVKLDIVALGSVLAVC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
VFYYTNALLYRSLNMELVPLRSLVSIIAALMIVYSRTHWRGTAQVKVSQAVLLKSVVLTV
HHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
IAGYLLLLGSIGEGMKYFGALFPRVLALSLGFIAGMLLLLLLLSERAQRELKVFLHKHFY
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSKYDYRAQWLGLTERLANFESGDGLLRLVLSAYCDIFGVRGGALFLHQDGCGWFCATGI
HCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
QELEEVRVTIAADNPLIAYLRDRRWVFCSRDDNPEILEANCRWLRELKVSFAIPLFEGEA
HHHHHCEEEEECCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCC
LTGFIVLGEQVVPDEEYRYEDYDLMKAIARQASVAIQHQRLSEQLTQAKAMEAVGNLATF
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVHDLKNLAATVSLVVENAREHLDNPEFQKDMLSSLGNTTRKMHTLIGRLRNLGESELLH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHEEC
MRPVDLLALARHSARQVQGRAVTVQGTPEKVIGDEEELEKVLLNLFLNAVEASEPGTQIV
CCCHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
AEVGCADSPYLKVSDSGCGMSPLFIRNELFAPFKTTKQAGLGIGLYQCRQIVAAHGGRIE
HHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHCCCEEE
VCSVEGEGTVFTVWFPSPALGAERVITQPA
EEEECCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]