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The map label for this gene is ubiE

Identifier: 197117284

GI number: 197117284

Start: 1048157

End: 1048864

Strand: Reverse

Name: ubiE

Synonym: Gbem_0894

Alternate gene names: 197117284

Gene position: 1048864-1048157 (Counterclockwise)

Preceding gene: 197117288

Following gene: 197117282

Centisome position: 22.73

GC content: 61.3

Gene sequence:

>708_bases
ATGTACGCATTGTCGGAAAAGGGTGAACGTATACGCGCCATGTTCGGGAGCATCGCCCCGCGTTACGATCTTTTGAACAG
ACTTCTTTCCCTGGGTATCGACCGCCGCTGGCGCCGCTTCGCCGTAAAGAAGATCGGGCTTAACGGGTCCGGGCGCGTGC
TCGACGTCGCCACCGGCACCGGGGACGTGGCGCTGGAGATCGCTTCGCAGACCCCCGCCTCGGTCAGCATCGTCGGCATC
GACTTCACCCCCGAGATGATCGAACTGGGGCGCGTGAAGGTAAAGGATTCGCGCCATTGCGGCCGCATTACGCTGCAGGT
GGCGCCTTGCGAAGAGATCCCCTTCGACGACGGGAGCTTCGACGCAGCCACCATCTCCTTCGGGATCAGGAACGTGGTGG
ACCGGATCAAGGGACTCGCCGAGATGCACCGGGTGCTGAAAAACGACGGCAAGATCGTCATCCTGGAGTTCTCCACCCCC
ACCCTCCCCGTGTTCAAGGATCTGTACCACTTCTACTTCCTGAAGGTGCTCCCGAAGATCGGCGGCGCCTTCTCCAGGTT
CAGCGCCTACCAATACCTGCCGGATTCCGTCCTGGAGTTCCCCTCCAGGGAGGTGTTCAAAGGGATGATGACTCAGGTGG
GATTCAAGGACGTGCGGCACTTCGACCTGACCGGCGGCATCGCCACCGTCTACGTCGGCACGAAGTAG

Upstream 100 bases:

>100_bases
AGGGGGCGCTTTATGTTAAAAATCAAAGCTCGCTAACCCTGTCCGCTAGGGGAAGGGGCTAAAATTTATAGCATTTCACG
CACCAAAAAGGTAGGAAAAT

Downstream 100 bases:

>100_bases
ATAATAGATAAGGTAGATAATCGTTCCATGGGTTGAGGCCGGGAGCTGCATGCTCCCACTCAACCCTTTTTTTACAGCCG
TTTTTGAAACAGCACCCGCG

Product: ubiquinone/menaquionone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MYALSEKGERIRAMFGSIAPRYDLLNRLLSLGIDRRWRRFAVKKIGLNGSGRVLDVATGTGDVALEIASQTPASVSIVGI
DFTPEMIELGRVKVKDSRHCGRITLQVAPCEEIPFDDGSFDAATISFGIRNVVDRIKGLAEMHRVLKNDGKIVILEFSTP
TLPVFKDLYHFYFLKVLPKIGGAFSRFSAYQYLPDSVLEFPSREVFKGMMTQVGFKDVRHFDLTGGIATVYVGTK

Sequences:

>Translated_235_residues
MYALSEKGERIRAMFGSIAPRYDLLNRLLSLGIDRRWRRFAVKKIGLNGSGRVLDVATGTGDVALEIASQTPASVSIVGI
DFTPEMIELGRVKVKDSRHCGRITLQVAPCEEIPFDDGSFDAATISFGIRNVVDRIKGLAEMHRVLKNDGKIVILEFSTP
TLPVFKDLYHFYFLKVLPKIGGAFSRFSAYQYLPDSVLEFPSREVFKGMMTQVGFKDVRHFDLTGGIATVYVGTK
>Mature_235_residues
MYALSEKGERIRAMFGSIAPRYDLLNRLLSLGIDRRWRRFAVKKIGLNGSGRVLDVATGTGDVALEIASQTPASVSIVGI
DFTPEMIELGRVKVKDSRHCGRITLQVAPCEEIPFDDGSFDAATISFGIRNVVDRIKGLAEMHRVLKNDGKIVILEFSTP
TLPVFKDLYHFYFLKVLPKIGGAFSRFSAYQYLPDSVLEFPSREVFKGMMTQVGFKDVRHFDLTGGIATVYVGTK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=265, Percent_Identity=33.9622641509434, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI48994983, Length=234, Percent_Identity=35.8974358974359, Blast_Score=167, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17557039, Length=234, Percent_Identity=32.9059829059829, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323526, Length=249, Percent_Identity=34.136546184739, Blast_Score=121, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24641702, Length=247, Percent_Identity=35.2226720647773, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_GEOBB (B5EFL1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137711.1
- ProteinModelPortal:   B5EFL1
- GeneID:   6783731
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0894
- HOGENOM:   HBG559580
- OMA:   GGAISKN
- ProtClustDB:   CLSK828068
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 26159; Mature: 26159

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYALSEKGERIRAMFGSIAPRYDLLNRLLSLGIDRRWRRFAVKKIGLNGSGRVLDVATGT
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
GDVALEIASQTPASVSIVGIDFTPEMIELGRVKVKDSRHCGRITLQVAPCEEIPFDDGSF
CCEEEEECCCCCCEEEEEEECCCHHHHHHCCEEEECCCCCCEEEEEEECCCCCCCCCCCC
DAATISFGIRNVVDRIKGLAEMHRVLKNDGKIVILEFSTPTLPVFKDLYHFYFLKVLPKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GGAFSRFSAYQYLPDSVLEFPSREVFKGMMTQVGFKDVRHFDLTGGIATVYVGTK
CCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCHHHHHEEECCCCEEEEEEECC
>Mature Secondary Structure
MYALSEKGERIRAMFGSIAPRYDLLNRLLSLGIDRRWRRFAVKKIGLNGSGRVLDVATGT
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
GDVALEIASQTPASVSIVGIDFTPEMIELGRVKVKDSRHCGRITLQVAPCEEIPFDDGSF
CCEEEEECCCCCCEEEEEEECCCHHHHHHCCEEEECCCCCCEEEEEEECCCCCCCCCCCC
DAATISFGIRNVVDRIKGLAEMHRVLKNDGKIVILEFSTPTLPVFKDLYHFYFLKVLPKI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GGAFSRFSAYQYLPDSVLEFPSREVFKGMMTQVGFKDVRHFDLTGGIATVYVGTK
CCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHCHHHHHEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA