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The map label for this gene is bioH [H]

Identifier: 197117224

GI number: 197117224

Start: 983303

End: 984103

Strand: Direct

Name: bioH [H]

Synonym: Gbem_0832

Alternate gene names: 197117224

Gene position: 983303-984103 (Clockwise)

Preceding gene: 197117223

Following gene: 197117225

Centisome position: 21.31

GC content: 65.42

Gene sequence:

>801_bases
ATGCCCTTCATCGAAACATCATCAGGACTCGCCCTTCACTACCAGGAAACAGGAGCCGGACGCCCTGTGGTATTCCTGCA
CGGCTGGGCCATGTCGGGGCGCGTCTGGCGCTTCCAGCATCCTCTGGACGACGCCTATCGGCTCATCTTCTTCGACCAGC
GCGGCCATGGACAGTCGGCTGCCGCCGAGGGCTATGCCATAGACGACTACGCGGGGGACGTGGCGGCGCTCTTTAGCCGG
CTCGCTCTGGAAGATGCCGTCCTCATCGGCTGGTCCCTAGGGGTGCAGGTGGCGTTGCAGGCCTTTCCATCTGTCAGGGA
GCGGCTCGCCGGGCTGGTGCTGGTGGGGGGCACCGCTCGTTTCACCACGGCGGAGGATTACCCGCACGGCAAACCCCCGG
TCGACGTGAAGGGGCTGAGCCTCAAGCTGCGGCGCGACTACCAAAAGACCATGGGGGACTTCTTCAAGGGGATGTTCGCG
GAAGGGGAGATGGACCAGGCGCAGTACCAGCGGATCGTGATGGGCGGCCGCTCCCCCAACACCTGTGCGGCGAAGGAGTC
GCTGAACATCCTGGCGACGGTGGACCAGAGGGACCGGCTGGCCCAGGTGGACCGCCCGGTACTGCTGGTGCACGGCGAGC
TGGATACCATCTGCCCCGCCGCGGCATCGGCATACATGGCAAAGCGGATGCCGCAGGCGAGGCTGGAGCTGGTCCCCGGA
TGCGGACACGCGCCTTTCATGACGCGGCCGGAGGGGTTCAACGCGCTGGTCAGGAATTTCATCGAGGGGTTGCGGAAGTA
A

Upstream 100 bases:

>100_bases
AAGCACAACTTCAGGGGAAACCTGAACGACGGGAAAACCCTCAGTGACCTCCGCGACCTCTGTGTTCCGCCTTTTGGTTT
TGAATTAGATTGGACAAGCT

Downstream 100 bases:

>100_bases
AGGCATCCCCCTCCCTGTCCCTCCCCCGCTGGGGGGGAGGGGACGTTGAGCAACTGCTCTTTCTCCAACTGGAGGGGGAC
GTTGAGCAACTGCTCTTTTC

Product: biotin biosynthesis carboxylesterase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPFIETSSGLALHYQETGAGRPVVFLHGWAMSGRVWRFQHPLDDAYRLIFFDQRGHGQSAAAEGYAIDDYAGDVAALFSR
LALEDAVLIGWSLGVQVALQAFPSVRERLAGLVLVGGTARFTTAEDYPHGKPPVDVKGLSLKLRRDYQKTMGDFFKGMFA
EGEMDQAQYQRIVMGGRSPNTCAAKESLNILATVDQRDRLAQVDRPVLLVHGELDTICPAAASAYMAKRMPQARLELVPG
CGHAPFMTRPEGFNALVRNFIEGLRK

Sequences:

>Translated_266_residues
MPFIETSSGLALHYQETGAGRPVVFLHGWAMSGRVWRFQHPLDDAYRLIFFDQRGHGQSAAAEGYAIDDYAGDVAALFSR
LALEDAVLIGWSLGVQVALQAFPSVRERLAGLVLVGGTARFTTAEDYPHGKPPVDVKGLSLKLRRDYQKTMGDFFKGMFA
EGEMDQAQYQRIVMGGRSPNTCAAKESLNILATVDQRDRLAQVDRPVLLVHGELDTICPAAASAYMAKRMPQARLELVPG
CGHAPFMTRPEGFNALVRNFIEGLRK
>Mature_265_residues
PFIETSSGLALHYQETGAGRPVVFLHGWAMSGRVWRFQHPLDDAYRLIFFDQRGHGQSAAAEGYAIDDYAGDVAALFSRL
ALEDAVLIGWSLGVQVALQAFPSVRERLAGLVLVGGTARFTTAEDYPHGKPPVDVKGLSLKLRRDYQKTMGDFFKGMFAE
GEMDQAQYQRIVMGGRSPNTCAAKESLNILATVDQRDRLAQVDRPVLLVHGELDTICPAAASAYMAKRMPQARLELVPGC
GHAPFMTRPEGFNALVRNFIEGLRK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=249, Percent_Identity=30.1204819277108, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 29271; Mature: 29140

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFIETSSGLALHYQETGAGRPVVFLHGWAMSGRVWRFQHPLDDAYRLIFFDQRGHGQSA
CCCEECCCCEEEEEECCCCCCCEEEEEEEEECCEEEEECCCCCCCEEEEEEECCCCCCCC
AAEGYAIDDYAGDVAALFSRLALEDAVLIGWSLGVQVALQAFPSVRERLAGLVLVGGTAR
CCCCEEECCCCCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCEE
FTTAEDYPHGKPPVDVKGLSLKLRRDYQKTMGDFFKGMFAEGEMDQAQYQRIVMGGRSPN
EEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
TCAAKESLNILATVDQRDRLAQVDRPVLLVHGELDTICPAAASAYMAKRMPQARLELVPG
CCHHHCCCEEEEECCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHEECCC
CGHAPFMTRPEGFNALVRNFIEGLRK
CCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFIETSSGLALHYQETGAGRPVVFLHGWAMSGRVWRFQHPLDDAYRLIFFDQRGHGQSA
CCEECCCCEEEEEECCCCCCCEEEEEEEEECCEEEEECCCCCCCEEEEEEECCCCCCCC
AAEGYAIDDYAGDVAALFSRLALEDAVLIGWSLGVQVALQAFPSVRERLAGLVLVGGTAR
CCCCEEECCCCCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCEE
FTTAEDYPHGKPPVDVKGLSLKLRRDYQKTMGDFFKGMFAEGEMDQAQYQRIVMGGRSPN
EEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
TCAAKESLNILATVDQRDRLAQVDRPVLLVHGELDTICPAAASAYMAKRMPQARLELVPG
CCHHHCCCEEEEECCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHEECCC
CGHAPFMTRPEGFNALVRNFIEGLRK
CCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]