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The map label for this gene is bioF

Identifier: 197117223

GI number: 197117223

Start: 981964

End: 983133

Strand: Direct

Name: bioF

Synonym: Gbem_0831

Alternate gene names: 197117223

Gene position: 981964-983133 (Clockwise)

Preceding gene: 197117219

Following gene: 197117224

Centisome position: 21.28

GC content: 64.96

Gene sequence:

>1170_bases
GTGCAGACATTTGCTGAAGAGCTTGAAGCCCTGCGGGCGGAGGGACTGTACCGCAGCATGAGGGTCATCAAAGGCGCACA
GGGAAGCCGGGTGGAGCTCGACGGGAAGCAGGTTCTCATGCTCTGCTCCAACAACTACCTGGGGCTTGCCGATCACCCCG
AGCTGAGAAGCGCCGCAGTATTCGGTGTGGCCTTCGGCGTCGGCAGCGGCGCCTCGCGGCTGGTTTCCGGCACCATGGAG
CTGCACGAAAAGCTTGAGGAGCGGATCGCCGCCTTCAAGGGGACCGAGAAGGCCCTGGTGTTCAACTCCGGCTACGCCGC
CAATACCGGTATCGTTTCCGCCCTCGTCGGGCGTGGCGACGCCATCTTCTCGGACCGGCTCAATCACGCCAGCATCATCG
ACGGCGCTCTTCTCTCCCGGGCCGATTTGCACCGCTACCCGCACCGTGACATGGCGGCCCTGGAGCGGCTGCTGCAGGAC
AAAGGCGGCAACGGCCGGCGCCTCATCGTCACCGACGGGGTGTTCAGCATGGACGGCGACATCGCCCCGCTGCAGGACCT
CGTGCGGCTGGCCAAGAAGTACGGCGCCCTGCTCATGGTCGACGACGCCCACGGCACAGGGGTACTAGGCCCGACCGGGC
GCGGCAGCGGCGAACTGCTGGGCGTCATGGACGGGATCGACATCCACATGGGAACCCTCGGCAAGGGGCTCGGAAGCTTC
GGCGCCTACGCGGCTGCGTCCGCAACCATCTGCGATTACCTGGTGAACAAGGCGCGCAGCTTCATCTTCTCTACCTCGCT
CCCCCCGGCCGTGCTGGCCGCCTCCATTGCGGCCATCGAACTGGTGGATTCGCCGGAAGGAAAAGAGCTGAGGGAGAAGC
TTGCGGCGAACGTCGCGCTCTTCAAGGAGAAGCTGGCGCAGGCGGGTTTCGACACCATGGGGAGCGAGACGCAGATAGTT
CCCATCTTCGTGGGACCGGCCGACGCCACCATGGAATTCAGCAAGGTACTCCTGGAGCAGGGGATCTTCGTACAGGGGAT
CAGGCCGCCCACGGTCCCGTCGGGGAGCTGCCGGCTGCGCTGTACCATCATGGCGACGCACGAACCGGCCGAACTGGAAG
AAGCCGCCGGAATCATCGAGCAGGTGGGGAAGAAGCTCGGGGTGGTTTAA

Upstream 100 bases:

>100_bases
GTCAACCTGTGTTGGTTGTCAGGTTGACGAACCCGGAGGTCCTGTGTTAATAACAATGGTCGGCTGGGAACCGGGGAATC
TGTCATAGGAGGGAGAGGCT

Downstream 100 bases:

>100_bases
ACAAAACTTCACTAAACCAAAGCTCACCACAGAGGTTCACAGAGGAAAAGACAAAGCGGACACAGAGGAAAGCACAACTT
CAGGGGAAACCTGAACGACG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MQTFAEELEALRAEGLYRSMRVIKGAQGSRVELDGKQVLMLCSNNYLGLADHPELRSAAVFGVAFGVGSGASRLVSGTME
LHEKLEERIAAFKGTEKALVFNSGYAANTGIVSALVGRGDAIFSDRLNHASIIDGALLSRADLHRYPHRDMAALERLLQD
KGGNGRRLIVTDGVFSMDGDIAPLQDLVRLAKKYGALLMVDDAHGTGVLGPTGRGSGELLGVMDGIDIHMGTLGKGLGSF
GAYAAASATICDYLVNKARSFIFSTSLPPAVLAASIAAIELVDSPEGKELREKLAANVALFKEKLAQAGFDTMGSETQIV
PIFVGPADATMEFSKVLLEQGIFVQGIRPPTVPSGSCRLRCTIMATHEPAELEEAAGIIEQVGKKLGVV

Sequences:

>Translated_389_residues
MQTFAEELEALRAEGLYRSMRVIKGAQGSRVELDGKQVLMLCSNNYLGLADHPELRSAAVFGVAFGVGSGASRLVSGTME
LHEKLEERIAAFKGTEKALVFNSGYAANTGIVSALVGRGDAIFSDRLNHASIIDGALLSRADLHRYPHRDMAALERLLQD
KGGNGRRLIVTDGVFSMDGDIAPLQDLVRLAKKYGALLMVDDAHGTGVLGPTGRGSGELLGVMDGIDIHMGTLGKGLGSF
GAYAAASATICDYLVNKARSFIFSTSLPPAVLAASIAAIELVDSPEGKELREKLAANVALFKEKLAQAGFDTMGSETQIV
PIFVGPADATMEFSKVLLEQGIFVQGIRPPTVPSGSCRLRCTIMATHEPAELEEAAGIIEQVGKKLGVV
>Mature_389_residues
MQTFAEELEALRAEGLYRSMRVIKGAQGSRVELDGKQVLMLCSNNYLGLADHPELRSAAVFGVAFGVGSGASRLVSGTME
LHEKLEERIAAFKGTEKALVFNSGYAANTGIVSALVGRGDAIFSDRLNHASIIDGALLSRADLHRYPHRDMAALERLLQD
KGGNGRRLIVTDGVFSMDGDIAPLQDLVRLAKKYGALLMVDDAHGTGVLGPTGRGSGELLGVMDGIDIHMGTLGKGLGSF
GAYAAASATICDYLVNKARSFIFSTSLPPAVLAASIAAIELVDSPEGKELREKLAANVALFKEKLAQAGFDTMGSETQIV
PIFVGPADATMEFSKVLLEQGIFVQGIRPPTVPSGSCRLRCTIMATHEPAELEEAAGIIEQVGKKLGVV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=387, Percent_Identity=42.1188630490956, Blast_Score=301, Evalue=9e-82,
Organism=Homo sapiens, GI284448556, Length=413, Percent_Identity=39.4673123486683, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI83977440, Length=385, Percent_Identity=34.025974025974, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI83977442, Length=385, Percent_Identity=34.025974025974, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI83977444, Length=385, Percent_Identity=34.025974025974, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI4502025, Length=359, Percent_Identity=36.2116991643454, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI40316939, Length=359, Percent_Identity=36.2116991643454, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4758668, Length=368, Percent_Identity=35.3260869565217, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI119220554, Length=368, Percent_Identity=33.1521739130435, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI5454084, Length=393, Percent_Identity=30.7888040712468, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1790046, Length=393, Percent_Identity=44.0203562340967, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=42.4929178470255, Blast_Score=254, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI71994529, Length=390, Percent_Identity=40, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32566772, Length=426, Percent_Identity=29.81220657277, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=30.4945054945055, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560914, Length=364, Percent_Identity=30.4945054945055, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71982625, Length=271, Percent_Identity=29.520295202952, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982617, Length=271, Percent_Identity=29.520295202952, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320438, Length=391, Percent_Identity=34.0153452685422, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320267, Length=368, Percent_Identity=33.1521739130435, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323954, Length=261, Percent_Identity=31.4176245210728, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24662918, Length=388, Percent_Identity=39.6907216494845, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI17137420, Length=369, Percent_Identity=34.4173441734417, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI17136286, Length=375, Percent_Identity=32.2666666666667, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24653280, Length=393, Percent_Identity=29.5165394402036, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24653276, Length=393, Percent_Identity=29.5165394402036, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24653278, Length=393, Percent_Identity=29.5165394402036, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_GEOBB (B5EEV8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137650.1
- ProteinModelPortal:   B5EEV8
- GeneID:   6783669
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0831
- HOGENOM:   HBG649839
- OMA:   QGIRPPT
- ProtClustDB:   CLSK828920
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41130; Mature: 41130

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 18-18 BINDING 129-129 BINDING 176-176 BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTFAEELEALRAEGLYRSMRVIKGAQGSRVELDGKQVLMLCSNNYLGLADHPELRSAAV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCEECCCCCCCHHHHHH
FGVAFGVGSGASRLVSGTMELHEKLEERIAAFKGTEKALVFNSGYAANTGIVSALVGRGD
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCC
AIFSDRLNHASIIDGALLSRADLHRYPHRDMAALERLLQDKGGNGRRLIVTDGVFSMDGD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCEEECCCC
IAPLQDLVRLAKKYGALLMVDDAHGTGVLGPTGRGSGELLGVMDGIDIHMGTLGKGLGSF
CHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHCCHHHH
GAYAAASATICDYLVNKARSFIFSTSLPPAVLAASIAAIELVDSPEGKELREKLAANVAL
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHH
FKEKLAQAGFDTMGSETQIVPIFVGPADATMEFSKVLLEQGIFVQGIRPPTVPSGSCRLR
HHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
CTIMATHEPAELEEAAGIIEQVGKKLGVV
EEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTFAEELEALRAEGLYRSMRVIKGAQGSRVELDGKQVLMLCSNNYLGLADHPELRSAAV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCEECCCCCCCHHHHHH
FGVAFGVGSGASRLVSGTMELHEKLEERIAAFKGTEKALVFNSGYAANTGIVSALVGRGD
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCC
AIFSDRLNHASIIDGALLSRADLHRYPHRDMAALERLLQDKGGNGRRLIVTDGVFSMDGD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCEEECCCC
IAPLQDLVRLAKKYGALLMVDDAHGTGVLGPTGRGSGELLGVMDGIDIHMGTLGKGLGSF
CHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHCCHHHH
GAYAAASATICDYLVNKARSFIFSTSLPPAVLAASIAAIELVDSPEGKELREKLAANVAL
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHH
FKEKLAQAGFDTMGSETQIVPIFVGPADATMEFSKVLLEQGIFVQGIRPPTVPSGSCRLR
HHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
CTIMATHEPAELEEAAGIIEQVGKKLGVV
EEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA