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The map label for this gene is pleD [H]

Identifier: 197117191

GI number: 197117191

Start: 939011

End: 939688

Strand: Reverse

Name: pleD [H]

Synonym: Gbem_0799

Alternate gene names: 197117191

Gene position: 939688-939011 (Counterclockwise)

Preceding gene: 197117192

Following gene: 308535194

Centisome position: 20.36

GC content: 60.32

Gene sequence:

>678_bases
ATGACTAGAGCATCGAGGATACTGATAGTGGATGACGAGCCGATCAACCTGCGGCTGGTATCGGTCTCTCTGGGCAACGA
CTACGAGGTGGTGACCGCGCTCAACGGGCACGAGGCTATCAGCAGGCTGAAGGAACAGCAGATCGACCTGATCCTGCTGG
ACGTGATGATGCCCGACATCAGCGGTTTCGACGTCTGCAAGATCATACAGGCCAATCCCGACTTCGTAGACATTCCGGTC
ATCTTCCTGACCGCCATGGACACCCCCCAGGGGGCGCGCCAGGGGCTCGCGGTCGGGGCCATCGACTACCTCACCAAGCC
GGTCGACCTGGAGCTTTTGAAGCTGCGGGTCCGCAACCTGGTCTCGCTCAAACAGCGCACCGACCTGGTGCGGGAGCAGC
GCGACCTGCTGGCACGGCAGAAGGAGGAACTGGAGGCCGCGCTGGCACGGGTGAGACAGCTGGAAGGGATCATCCCCATC
TGCATGTTCTGCAAGAAGATCAAGGACGACCAGGAGAGCTGGCGGCAACTGGAGGACTACGTAAGCCAGCACTCGGAGGT
GATGTTCAGCCACAGCGCCTGTCCTGAATGCTTTGAAAAAGAGATGAACTCTATCAAGGAGGAACTGTCAGCGTTAGGCC
TCCCCCCGGATCCCGGGTCTAAGCCTGCGCCCCCCTAG

Upstream 100 bases:

>100_bases
TTCAGGGAGGAGGGGTTTGACGGCTACCTCTCCAAGCCGCTGGAGACCGGCGAGCTGATCCAGGAATTGCAGCGGGTCCT
GTACGCCGTGGAGGTGAGTC

Downstream 100 bases:

>100_bases
CATAAGACAACGGCAGAGAGCTTCAGTCGATGCTGCCTCCCGGCACTGCCACGGCCGCCCCTCTCAGATGCAGGACAAAT
CGAAGCTCCCTTGCCGAAAA

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTRASRILIVDDEPINLRLVSVSLGNDYEVVTALNGHEAISRLKEQQIDLILLDVMMPDISGFDVCKIIQANPDFVDIPV
IFLTAMDTPQGARQGLAVGAIDYLTKPVDLELLKLRVRNLVSLKQRTDLVREQRDLLARQKEELEAALARVRQLEGIIPI
CMFCKKIKDDQESWRQLEDYVSQHSEVMFSHSACPECFEKEMNSIKEELSALGLPPDPGSKPAPP

Sequences:

>Translated_225_residues
MTRASRILIVDDEPINLRLVSVSLGNDYEVVTALNGHEAISRLKEQQIDLILLDVMMPDISGFDVCKIIQANPDFVDIPV
IFLTAMDTPQGARQGLAVGAIDYLTKPVDLELLKLRVRNLVSLKQRTDLVREQRDLLARQKEELEAALARVRQLEGIIPI
CMFCKKIKDDQESWRQLEDYVSQHSEVMFSHSACPECFEKEMNSIKEELSALGLPPDPGSKPAPP
>Mature_224_residues
TRASRILIVDDEPINLRLVSVSLGNDYEVVTALNGHEAISRLKEQQIDLILLDVMMPDISGFDVCKIIQANPDFVDIPVI
FLTAMDTPQGARQGLAVGAIDYLTKPVDLELLKLRVRNLVSLKQRTDLVREQRDLLARQKEELEAALARVRQLEGIIPIC
MFCKKIKDDQESWRQLEDYVSQHSEVMFSHSACPECFEKEMNSIKEELSALGLPPDPGSKPAPP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=119, Percent_Identity=36.1344537815126, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=139, Percent_Identity=32.3741007194245, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=38.1818181818182, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=37.8151260504202, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790863, Length=131, Percent_Identity=34.3511450381679, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=130, Percent_Identity=34.6153846153846, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=35.5932203389831, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=127, Percent_Identity=33.8582677165354, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 25308; Mature: 25176

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRASRILIVDDEPINLRLVSVSLGNDYEVVTALNGHEAISRLKEQQIDLILLDVMMPDI
CCCCCEEEEECCCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCEEEHHCCCC
SGFDVCKIIQANPDFVDIPVIFLTAMDTPQGARQGLAVGAIDYLTKPVDLELLKLRVRNL
CCCCCEEEEECCCCEEEHHEEEEEECCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
VSLKQRTDLVREQRDLLARQKEELEAALARVRQLEGIIPICMFCKKIKDDQESWRQLEDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
VSQHSEVMFSHSACPECFEKEMNSIKEELSALGLPPDPGSKPAPP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRASRILIVDDEPINLRLVSVSLGNDYEVVTALNGHEAISRLKEQQIDLILLDVMMPDI
CCCCEEEEECCCCCEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCEEEHHCCCC
SGFDVCKIIQANPDFVDIPVIFLTAMDTPQGARQGLAVGAIDYLTKPVDLELLKLRVRNL
CCCCCEEEEECCCCEEEHHEEEEEECCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
VSLKQRTDLVREQRDLLARQKEELEAALARVRQLEGIIPICMFCKKIKDDQESWRQLEDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
VSQHSEVMFSHSACPECFEKEMNSIKEELSALGLPPDPGSKPAPP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA