Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 197117039

GI number: 197117039

Start: 762351

End: 764414

Strand: Direct

Name: glyS

Synonym: Gbem_0642

Alternate gene names: 197117039

Gene position: 762351-764414 (Clockwise)

Preceding gene: 308535180

Following gene: 197117040

Centisome position: 16.52

GC content: 64.87

Gene sequence:

>2064_bases
ATGGCTAAGGACCTGTTTCTGGAGATAGGTTGCGAAGAGATCCCGGCCGGCTTCGTCCCCAAGGCGATGGCCGACATGGA
GCTTTTGATCAAGAAAGAGTTCGACAGCGCCCGCATCGAGTACGGCGAGATCGTGACGCTCGGTACCCCGCGCCGCCTGG
TGCTGGCCGTTAAGGGGGTCGCCGAGAGGCAGCCTGACGCGGAACTGACCGCGATGGGGCCGGCCAAAAGCCACGCCTAC
GACGCCGACGGGAACCCCACCAAGGCAGCCCAGGGCTTCGCCCGCGGCCAGGGAATCGATGTCTCGCAGCTGAAGCTCGT
GACCACGGAGAAAGGCGAGTATCTCGCCGCCGTGAAAAGCGAGATAGGCCGCGCTACGGCAGAACTCCTCCCCGAACTCC
TGCCGCGCCTGATCGGGAACATCCCCTTCAAGAAGTCGATGCGCTGGGCCGACTTCGACGTCCGCTTCGCCCGCCCCATC
CACTGGATCGTGGCGCTCTTCGACGGCAAGGTGGTTCCCTTCTCCTTCGGCAACATCGACAGCGGAAGCGCCTCGCGCGG
CCACCGCTTCATGGCCAATACCTCCTTCCCGGTGCGCGACCTGGCCCACTACTTAGAGGAGTGCGAGCGCCATTTCGTCA
TCCCCGACCCGAATAAGCGCAAAGAGATCATCCGCGCCGAGATCGAGCGGGTGGCCAAGCAGGCCAAGGGGAACGTCCTT
CCCGACGAGGCTCTCCTGGAGCAGGTGAGCTACCTGGTCGAGTACCCGAGCGCGGTGCACGGCACCTTCTCACCCGATTT
CCTGGTGGTGCCGCGCGAGGTGCTGATCACCTCCATGCGCGAGCACCAGCGCTACTTCTCCCTGGTGGACGATGAGGGGA
AACTCCTCCCCGGCTTCATCACCATCAACAACACCATCACCGAGGACCCGCAGGTGGTGGTCAAGGGGAACGAGAGGGTG
CTCCGCGCCCGCCTCTCCGACGCCCGCTTCTTCTTCGACGAGGACCACAAGGTGAGGCTCGAAGCCCGCGTCGAGTCCCT
GAAGAGCGTGGTGTACCAGGCGAAGCTCGGCACCTCCTACGAGAAGATGGAGCGTTTCCGCGAGCTGGGCAAACGCCTGG
CCCAGAGGCTGAACCCCGCCGTGACCAAAGAGGTCGAGCGCGCGGCGACGCTTTGCAAGGCGGATCTCGTCTCCGGCATG
GTCGGGGAATTCCCCGAGGTGCAGGGGATCATGGGGCGCGAGTATGCGCTCCACGACGGCGAGGAGAAGGCTGTGGCCAA
CGCCATCGCCGAGCACTACCTCCCGACCCAGGCAGGGGGCGAGCTTCCCGCCTCCGACATCGGCGCCTTCGTCTCCTTGG
CCGACAAGATGGACACCATCTGCGGCTGCTTCTCGGTTGGGCTCATCCCCACCGGTTCCGCCGACCCCTACGCCCTCAGG
CGTTCGGCGCTCGGCATCATCAACATCATTCTGGACAAGGGGTACCGCGAGCCGCTCTCCGACTTCGTCAAGGCGTCGCT
CGATCTCCTCTCCGCCAAGGCGACCCGGCCGCTTGCCGAGGTGCAAAAGGACGTCCTCGACTTCTTCCGCGGGCGCTTCG
TGAACCTGATGGCGGACCGCTTCCCCTCCGACGCGGTGGAGGCGGTGGTGTCGGTCTCCTTCGACGACCTGGTCGAGGCG
GCAGCGAAGATCGAGGCACTGGCAGGCTTCCGTAACCGCGACGACTTCGGCCCCCTGGCCGTCGCCTTCAAAAGGGTCTG
CAACATCGTCAAGGACGGGGTGGATACCCCGGTCTCGACCGAACTCTTCCAGGACGCCGCCGAAGGTGAACTGCACCAGG
CGCTGACGCAGGTCTCCGGCAAAGTGGCGGCAGCGCTCAAAAAGGCCGACTACCTGGCGGCCCTGACCGAGATAGCGACG
CTGAAGCCCGCCGTCGACCTCTTCTTCGAAAAGGTCATGGTCATGGCCGAGGACGAACGGGTGCGCCAGAATCGCCTGGC
GCTTCTCACCGGCATAGCGAGGCTCTTCGGAAGCCTGGCGGACTTCTCGAGGTTGTCGCCCTGA

Upstream 100 bases:

>100_bases
CCTACATCGGCAAGGTGAGAAACGTGGCCAGGCTCTGCGCCGAGGGTTACCTGCAGATGCGCGAGCGGCTCGGTTTCCCG
CTGCTGAAAGGAGGGCTCTA

Downstream 100 bases:

>100_bases
TGATGAAGCTGCGGCAAAAGCAGATGAACGGGAACATGAAGACCACATCCGCAGCACAGAAGACCAAAAATAACGCTCAC
GTAAACAGCCGTACGCGCCT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MAKDLFLEIGCEEIPAGFVPKAMADMELLIKKEFDSARIEYGEIVTLGTPRRLVLAVKGVAERQPDAELTAMGPAKSHAY
DADGNPTKAAQGFARGQGIDVSQLKLVTTEKGEYLAAVKSEIGRATAELLPELLPRLIGNIPFKKSMRWADFDVRFARPI
HWIVALFDGKVVPFSFGNIDSGSASRGHRFMANTSFPVRDLAHYLEECERHFVIPDPNKRKEIIRAEIERVAKQAKGNVL
PDEALLEQVSYLVEYPSAVHGTFSPDFLVVPREVLITSMREHQRYFSLVDDEGKLLPGFITINNTITEDPQVVVKGNERV
LRARLSDARFFFDEDHKVRLEARVESLKSVVYQAKLGTSYEKMERFRELGKRLAQRLNPAVTKEVERAATLCKADLVSGM
VGEFPEVQGIMGREYALHDGEEKAVANAIAEHYLPTQAGGELPASDIGAFVSLADKMDTICGCFSVGLIPTGSADPYALR
RSALGIINIILDKGYREPLSDFVKASLDLLSAKATRPLAEVQKDVLDFFRGRFVNLMADRFPSDAVEAVVSVSFDDLVEA
AAKIEALAGFRNRDDFGPLAVAFKRVCNIVKDGVDTPVSTELFQDAAEGELHQALTQVSGKVAAALKKADYLAALTEIAT
LKPAVDLFFEKVMVMAEDERVRQNRLALLTGIARLFGSLADFSRLSP

Sequences:

>Translated_687_residues
MAKDLFLEIGCEEIPAGFVPKAMADMELLIKKEFDSARIEYGEIVTLGTPRRLVLAVKGVAERQPDAELTAMGPAKSHAY
DADGNPTKAAQGFARGQGIDVSQLKLVTTEKGEYLAAVKSEIGRATAELLPELLPRLIGNIPFKKSMRWADFDVRFARPI
HWIVALFDGKVVPFSFGNIDSGSASRGHRFMANTSFPVRDLAHYLEECERHFVIPDPNKRKEIIRAEIERVAKQAKGNVL
PDEALLEQVSYLVEYPSAVHGTFSPDFLVVPREVLITSMREHQRYFSLVDDEGKLLPGFITINNTITEDPQVVVKGNERV
LRARLSDARFFFDEDHKVRLEARVESLKSVVYQAKLGTSYEKMERFRELGKRLAQRLNPAVTKEVERAATLCKADLVSGM
VGEFPEVQGIMGREYALHDGEEKAVANAIAEHYLPTQAGGELPASDIGAFVSLADKMDTICGCFSVGLIPTGSADPYALR
RSALGIINIILDKGYREPLSDFVKASLDLLSAKATRPLAEVQKDVLDFFRGRFVNLMADRFPSDAVEAVVSVSFDDLVEA
AAKIEALAGFRNRDDFGPLAVAFKRVCNIVKDGVDTPVSTELFQDAAEGELHQALTQVSGKVAAALKKADYLAALTEIAT
LKPAVDLFFEKVMVMAEDERVRQNRLALLTGIARLFGSLADFSRLSP
>Mature_686_residues
AKDLFLEIGCEEIPAGFVPKAMADMELLIKKEFDSARIEYGEIVTLGTPRRLVLAVKGVAERQPDAELTAMGPAKSHAYD
ADGNPTKAAQGFARGQGIDVSQLKLVTTEKGEYLAAVKSEIGRATAELLPELLPRLIGNIPFKKSMRWADFDVRFARPIH
WIVALFDGKVVPFSFGNIDSGSASRGHRFMANTSFPVRDLAHYLEECERHFVIPDPNKRKEIIRAEIERVAKQAKGNVLP
DEALLEQVSYLVEYPSAVHGTFSPDFLVVPREVLITSMREHQRYFSLVDDEGKLLPGFITINNTITEDPQVVVKGNERVL
RARLSDARFFFDEDHKVRLEARVESLKSVVYQAKLGTSYEKMERFRELGKRLAQRLNPAVTKEVERAATLCKADLVSGMV
GEFPEVQGIMGREYALHDGEEKAVANAIAEHYLPTQAGGELPASDIGAFVSLADKMDTICGCFSVGLIPTGSADPYALRR
SALGIINIILDKGYREPLSDFVKASLDLLSAKATRPLAEVQKDVLDFFRGRFVNLMADRFPSDAVEAVVSVSFDDLVEAA
AKIEALAGFRNRDDFGPLAVAFKRVCNIVKDGVDTPVSTELFQDAAEGELHQALTQVSGKVAAALKKADYLAALTEIATL
KPAVDLFFEKVMVMAEDERVRQNRLALLTGIARLFGSLADFSRLSP

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=38.4615384615385, Blast_Score=458, Evalue=1e-130,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_GEOBB (B5ED84)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137466.1
- GeneID:   6783475
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0642
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75711; Mature: 75580

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDLFLEIGCEEIPAGFVPKAMADMELLIKKEFDSARIEYGEIVTLGTPRRLVLAVKGV
CCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEHEEHHH
AERQPDAELTAMGPAKSHAYDADGNPTKAAQGFARGQGIDVSQLKLVTTEKGEYLAAVKS
HCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHEEEEEECCCHHHHHHHH
EIGRATAELLPELLPRLIGNIPFKKSMRWADFDVRFARPIHWIVALFDGKVVPFSFGNID
HHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCC
SGSASRGHRFMANTSFPVRDLAHYLEECERHFVIPDPNKRKEIIRAEIERVAKQAKGNVL
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PDEALLEQVSYLVEYPSAVHGTFSPDFLVVPREVLITSMREHQRYFSLVDDEGKLLPGFI
CHHHHHHHHHHHHHCCCHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEECEEE
TINNTITEDPQVVVKGNERVLRARLSDARFFFDEDHKVRLEARVESLKSVVYQAKLGTSY
EECCCCCCCCEEEEECCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCH
EKMERFRELGKRLAQRLNPAVTKEVERAATLCKADLVSGMVGEFPEVQGIMGREYALHDG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
EEKAVANAIAEHYLPTQAGGELPASDIGAFVSLADKMDTICGCFSVGLIPTGSADPYALR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RSALGIINIILDKGYREPLSDFVKASLDLLSAKATRPLAEVQKDVLDFFRGRFVNLMADR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FPSDAVEAVVSVSFDDLVEAAAKIEALAGFRNRDDFGPLAVAFKRVCNIVKDGVDTPVST
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
ELFQDAAEGELHQALTQVSGKVAAALKKADYLAALTEIATLKPAVDLFFEKVMVMAEDER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRQNRLALLTGIARLFGSLADFSRLSP
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKDLFLEIGCEEIPAGFVPKAMADMELLIKKEFDSARIEYGEIVTLGTPRRLVLAVKGV
CCCCEEECCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEHEEHHH
AERQPDAELTAMGPAKSHAYDADGNPTKAAQGFARGQGIDVSQLKLVTTEKGEYLAAVKS
HCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHEEEEEECCCHHHHHHHH
EIGRATAELLPELLPRLIGNIPFKKSMRWADFDVRFARPIHWIVALFDGKVVPFSFGNID
HHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCC
SGSASRGHRFMANTSFPVRDLAHYLEECERHFVIPDPNKRKEIIRAEIERVAKQAKGNVL
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PDEALLEQVSYLVEYPSAVHGTFSPDFLVVPREVLITSMREHQRYFSLVDDEGKLLPGFI
CHHHHHHHHHHHHHCCCHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEECEEE
TINNTITEDPQVVVKGNERVLRARLSDARFFFDEDHKVRLEARVESLKSVVYQAKLGTSY
EECCCCCCCCEEEEECCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCH
EKMERFRELGKRLAQRLNPAVTKEVERAATLCKADLVSGMVGEFPEVQGIMGREYALHDG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
EEKAVANAIAEHYLPTQAGGELPASDIGAFVSLADKMDTICGCFSVGLIPTGSADPYALR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RSALGIINIILDKGYREPLSDFVKASLDLLSAKATRPLAEVQKDVLDFFRGRFVNLMADR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FPSDAVEAVVSVSFDDLVEAAAKIEALAGFRNRDDFGPLAVAFKRVCNIVKDGVDTPVST
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHH
ELFQDAAEGELHQALTQVSGKVAAALKKADYLAALTEIATLKPAVDLFFEKVMVMAEDER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRQNRLALLTGIARLFGSLADFSRLSP
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA