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The map label for this gene is merA [H]

Identifier: 197117037

GI number: 197117037

Start: 759821

End: 761227

Strand: Reverse

Name: merA [H]

Synonym: Gbem_0640

Alternate gene names: 197117037

Gene position: 761227-759821 (Counterclockwise)

Preceding gene: 197117047

Following gene: 197117036

Centisome position: 16.49

GC content: 67.66

Gene sequence:

>1407_bases
ATGAACCAGCAACCCGAAATCATCATCATAGGCTCCGGCTCCACCGCCTTTGCCGCAGCGCTCAGGGCGCAGGAGCGGGG
TGCAGCCTCCATCATGATCGAGCGAAGCGCCCTCGGCGGGACCTGCATCAACTGGGGGTGCATCCCCAGCAAGACCCTGA
TCCACGCGGCGCTCTTCCGGCACGAGGCGACGCTTGGCGAGCGGCTCGGGCTGGGGGCGCTGAGCCAGGCGCTCGACTTC
CCGCTTTTGGACTCCCACAAGCTGGACGTGGTGCAAAGGCTGAGAACGACCAAGTACCTGGACGTGCTCAAAAACGTCCC
GGGGCTCACCCTGGTCAAGGGGATGGCGGTCTTCACCTCCCCGGACACCGTCCGGGTCGGGGAGCGGCGCCTCACCGGGA
GCCGGTTCCTCATCGCCGCCGGGGGCGTCCCCCGCGTCCCCCCCATCGCAGGACTCGACGAGACCCCCTTCCTGACCAGC
AAGAGCGCGCTGATGCTGAAAAAAATCCCCGTATCGCTCACCATCATCGGCGGCGGCGTCATCGCCCTGGAACTGGGACA
GATGTTCCTTAGGCTGGGGGTAAAGGTAACCGTCCTTGAGCACGGGCAGAGGGTGCTTCCGGCGATCGAGGCGGAGCCCG
CCCTGGCGCTGCAGGACGTCCTCGCCTCCGAGGGGATGCGCATCATCCTGAACGCCTCGGTGCTGAGCGCCTGCCGGCAT
GGCGACGGCGTGAGGGTCGAGGCGCTGGTCGGGGGGGAACGCACCTGCCTTGATTCGCAGCAGCTCCTCGTGGCCGTCGG
CACCGCCCCCGCCACCGAAGGGCTTGGCCTGGAGCAGGCCGGGGTGCAGGTGGACCAAAGGGGGTTCATCGTGGTGGACG
AGCAGATGCGCACCTCCTCCCCCGGGATCTGGGCCGCAGGCGACGTCACCGGCCGGATGCAGATCGCCACCGTCGGCGCC
CGCGAGGGGATCGCCGCCGTGGACAACATGCTGGAGACCGGCTGCAACTGCGCCCTCGACTACCAGACCCTCCCTATGGC
CATCTTCACCGACCCGGAGGTCGGAACCGTCGGCTACACAGAAGAGGGAGCGCGCCGGGCCGGGTTCGAGGTAGAAAGCC
ACACCATCCCCGCCTCCGCCATCGCAAAGGCGCACGTCACCGGGGCGCTCGCCGGCGCCGTCAAGATCGTCGCCGAGACA
GGAACCGGAAGGATTCTGGGCGTGCACCTCTGCCTGCACCGCGGGGCCGACATCATCAACGAGGCCGCACTCGCCATCCG
TTGCCGCATGACCGTGGCGGAGCTTGCCGACACCCTCCACGTCTACCCCTCGATGGGTGAAGGGTTGCGGCTCTGCGCCC
AGGCCTTTAGCAGAAACCTGGACCATCTCTCCTGCTGCGCCGAATAG

Upstream 100 bases:

>100_bases
CTTTTACCCCGGTCAATGGGGAAAGGGTGCAAAGGTCTCTTTATTCTTCCCGTTTACCGAAACTGGCGTTCAAGGTACAC
TGAGGTGGGGGTAATTTCAG

Downstream 100 bases:

>100_bases
CCCAAGGCTCCCCCGCCCCCGCTTCCCATTAGTTTAAAATTGACAACAATTTCATGAAAGACTACTATGCCTCAAATTGG
TTGCGGCTAACGGACCGAGA

Product: dihydrolipoamide dehydrogenase-like protein

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNQQPEIIIIGSGSTAFAAALRAQERGAASIMIERSALGGTCINWGCIPSKTLIHAALFRHEATLGERLGLGALSQALDF
PLLDSHKLDVVQRLRTTKYLDVLKNVPGLTLVKGMAVFTSPDTVRVGERRLTGSRFLIAAGGVPRVPPIAGLDETPFLTS
KSALMLKKIPVSLTIIGGGVIALELGQMFLRLGVKVTVLEHGQRVLPAIEAEPALALQDVLASEGMRIILNASVLSACRH
GDGVRVEALVGGERTCLDSQQLLVAVGTAPATEGLGLEQAGVQVDQRGFIVVDEQMRTSSPGIWAAGDVTGRMQIATVGA
REGIAAVDNMLETGCNCALDYQTLPMAIFTDPEVGTVGYTEEGARRAGFEVESHTIPASAIAKAHVTGALAGAVKIVAET
GTGRILGVHLCLHRGADIINEAALAIRCRMTVAELADTLHVYPSMGEGLRLCAQAFSRNLDHLSCCAE

Sequences:

>Translated_468_residues
MNQQPEIIIIGSGSTAFAAALRAQERGAASIMIERSALGGTCINWGCIPSKTLIHAALFRHEATLGERLGLGALSQALDF
PLLDSHKLDVVQRLRTTKYLDVLKNVPGLTLVKGMAVFTSPDTVRVGERRLTGSRFLIAAGGVPRVPPIAGLDETPFLTS
KSALMLKKIPVSLTIIGGGVIALELGQMFLRLGVKVTVLEHGQRVLPAIEAEPALALQDVLASEGMRIILNASVLSACRH
GDGVRVEALVGGERTCLDSQQLLVAVGTAPATEGLGLEQAGVQVDQRGFIVVDEQMRTSSPGIWAAGDVTGRMQIATVGA
REGIAAVDNMLETGCNCALDYQTLPMAIFTDPEVGTVGYTEEGARRAGFEVESHTIPASAIAKAHVTGALAGAVKIVAET
GTGRILGVHLCLHRGADIINEAALAIRCRMTVAELADTLHVYPSMGEGLRLCAQAFSRNLDHLSCCAE
>Mature_468_residues
MNQQPEIIIIGSGSTAFAAALRAQERGAASIMIERSALGGTCINWGCIPSKTLIHAALFRHEATLGERLGLGALSQALDF
PLLDSHKLDVVQRLRTTKYLDVLKNVPGLTLVKGMAVFTSPDTVRVGERRLTGSRFLIAAGGVPRVPPIAGLDETPFLTS
KSALMLKKIPVSLTIIGGGVIALELGQMFLRLGVKVTVLEHGQRVLPAIEAEPALALQDVLASEGMRIILNASVLSACRH
GDGVRVEALVGGERTCLDSQQLLVAVGTAPATEGLGLEQAGVQVDQRGFIVVDEQMRTSSPGIWAAGDVTGRMQIATVGA
REGIAAVDNMLETGCNCALDYQTLPMAIFTDPEVGTVGYTEEGARRAGFEVESHTIPASAIAKAHVTGALAGAVKIVAET
GTGRILGVHLCLHRGADIINEAALAIRCRMTVAELADTLHVYPSMGEGLRLCAQAFSRNLDHLSCCAE

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=32.6271186440678, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=27.4891774891775, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=23.9316239316239, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI22035672, Length=438, Percent_Identity=27.1689497716895, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=23.9316239316239, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=23.9316239316239, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=23.9316239316239, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=23.9316239316239, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045266, Length=430, Percent_Identity=22.7906976744186, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=33.2603938730853, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=30.5555555555556, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=28.6046511627907, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87082354, Length=479, Percent_Identity=26.9311064718163, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=32.244008714597, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71983419, Length=346, Percent_Identity=29.4797687861272, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=346, Percent_Identity=29.4797687861272, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=24.2677824267782, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=34.5415778251599, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=25.6900212314225, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=24.8380129589633, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=33.8235294117647, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24640549, Length=501, Percent_Identity=26.3473053892216, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640553, Length=501, Percent_Identity=26.3473053892216, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640551, Length=501, Percent_Identity=26.3473053892216, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17737741, Length=507, Percent_Identity=25.6410256410256, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49228; Mature: 49228

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQPEIIIIGSGSTAFAAALRAQERGAASIMIERSALGGTCINWGCIPSKTLIHAALFR
CCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHH
HEATLGERLGLGALSQALDFPLLDSHKLDVVQRLRTTKYLDVLKNVPGLTLVKGMAVFTS
HHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHCEEEEEC
PDTVRVGERRLTGSRFLIAAGGVPRVPPIAGLDETPFLTSKSALMLKKIPVSLTIIGGGV
CCCEECCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCE
IALELGQMFLRLGVKVTVLEHGQRVLPAIEAEPALALQDVLASEGMRIILNASVLSACRH
EEHHHHHHHHHHCCEEEEEECCCEECCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHC
GDGVRVEALVGGERTCLDSQQLLVAVGTAPATEGLGLEQAGVQVDQRGFIVVDEQMRTSS
CCCCEEEEEECCCCEECCCCEEEEEEECCCCCCCCCCHHCCCEECCCCEEEEECCCCCCC
PGIWAAGDVTGRMQIATVGAREGIAAVDNMLETGCNCALDYQTLPMAIFTDPEVGTVGYT
CCEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCCC
EEGARRAGFEVESHTIPASAIAKAHVTGALAGAVKIVAETGTGRILGVHLCLHRGADIIN
CCCHHHCCCEEECCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEHHHHHCCHHHHH
EAALAIRCRMTVAELADTLHVYPSMGEGLRLCAQAFSRNLDHLSCCAE
HHHEEEEEHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MNQQPEIIIIGSGSTAFAAALRAQERGAASIMIERSALGGTCINWGCIPSKTLIHAALFR
CCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHH
HEATLGERLGLGALSQALDFPLLDSHKLDVVQRLRTTKYLDVLKNVPGLTLVKGMAVFTS
HHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHCEEEEEC
PDTVRVGERRLTGSRFLIAAGGVPRVPPIAGLDETPFLTSKSALMLKKIPVSLTIIGGGV
CCCEECCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCE
IALELGQMFLRLGVKVTVLEHGQRVLPAIEAEPALALQDVLASEGMRIILNASVLSACRH
EEHHHHHHHHHHCCEEEEEECCCEECCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHC
GDGVRVEALVGGERTCLDSQQLLVAVGTAPATEGLGLEQAGVQVDQRGFIVVDEQMRTSS
CCCCEEEEEECCCCEECCCCEEEEEEECCCCCCCCCCHHCCCEECCCCEEEEECCCCCCC
PGIWAAGDVTGRMQIATVGAREGIAAVDNMLETGCNCALDYQTLPMAIFTDPEVGTVGYT
CCEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCCC
EEGARRAGFEVESHTIPASAIAKAHVTGALAGAVKIVAETGTGRILGVHLCLHRGADIIN
CCCHHHCCCEEECCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEHHHHHCCHHHHH
EAALAIRCRMTVAELADTLHVYPSMGEGLRLCAQAFSRNLDHLSCCAE
HHHEEEEEHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]