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The map label for this gene is rpfG [H]

Identifier: 197117036

GI number: 197117036

Start: 758637

End: 759674

Strand: Reverse

Name: rpfG [H]

Synonym: Gbem_0639

Alternate gene names: 197117036

Gene position: 759674-758637 (Counterclockwise)

Preceding gene: 197117037

Following gene: 197117030

Centisome position: 16.46

GC content: 63.68

Gene sequence:

>1038_bases
ATGGCGGCAGAGATACTCTTTGTGGACGACAACAAACTGGTACTCCAGGCGCTGAGCGACCTACTGAGGTCGCACGGCAT
CGCCCCCCTCGCCGCCAGCGACGCGGAAGAGGCCCTGGAACTCTTCAAAAAGCACGAGATAGCGGTGGTGGTCTCGGACA
ACCAGATGCCGGGGATGTCGGGGCTGGAGTTCCTGCGCGAGCTCAGGAACGTCTCGCCGGACACCGTGAAGGTACTCATC
TCCTCCCACGTCGACCTCCCGACGGCGCTTGCCGCCATCAACAGTTCCGAGGTCTACCGCTGTATGATGAAACCGTGGAA
GGACGAGGAGATCGTCGAGGTGGTGCAGGAGGGGTTGAGGCGCTACCGCACCATTCATGACATACGGCGCGAGGACGAGG
CGGTGCTCCGTTCCCTGGCGCAGACCATTGAGCTCAAGGACCCGAGCACCAAGGGGCACTGCGACCGTGTCGCGGTCTAC
GCGCTTTTGGTCGCCGAGGCGATGGGGATAGCGAAGGAGATGCAGCGGCAGATCAAGTACGGAAGCTGGCTGCACGACTG
CGGCAAGATCGGGGTTTCGGAGTTCATACTGAACGGTCCGGGCAAGTTGAGCGAGGACGAGTTCGAGACCATGAAGATGC
ACACCGACTGGGGGGTGGACGTGGCGGAAAAAGCCGACCTGTCCCAGGTGGCGCGGAACATCATCCACTACCATCACGAG
AAGTACGACGGCAGCGGCTACCCCTGCGGGCTCATGGGGGAGGAGATTCCCATCGAAGCGCGCATCGTTTCGGTGGCCGA
CGTCTACGACGCCCTCACCTCGGACCGGTCCTACCGCAAGGCATATACCTTCGAGAAGGCGCGCCAGATGGTGCGCGAGA
TGGCGGGCACGGCGCTGGACCCGGCGGTGGTCGAGGTGTTCCTGGCCGTGGTCCCCGACGGGCCGCTACCGTCGAGCGAG
GAGTTCCCGGAGTGCGACTCCCACCGAGCGGGCGGGAAGAAGCCCCTCAACCTGGTGCTTCCGGCCGCTACCTGCTGA

Upstream 100 bases:

>100_bases
CAATTTCATGAAAGACTACTATGCCTCAAATTGGTTGCGGCTAACGGACCGAGACGGTTCCGCGCCGCATCAGCGTCAGG
CGCAGACGGGGGCGGTGAAG

Downstream 100 bases:

>100_bases
TTGCCGCGCGGCCCGGCTAGCGAAGCCAGCGGCGCACCACGAAGCTCTTCACCCCGGCAGCCCCCTTCGGATACCGGTCC
GCCGGCTCCCTGGTCCGCAT

Product: response receiver-modulated cyclic diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAAEILFVDDNKLVLQALSDLLRSHGIAPLAASDAEEALELFKKHEIAVVVSDNQMPGMSGLEFLRELRNVSPDTVKVLI
SSHVDLPTALAAINSSEVYRCMMKPWKDEEIVEVVQEGLRRYRTIHDIRREDEAVLRSLAQTIELKDPSTKGHCDRVAVY
ALLVAEAMGIAKEMQRQIKYGSWLHDCGKIGVSEFILNGPGKLSEDEFETMKMHTDWGVDVAEKADLSQVARNIIHYHHE
KYDGSGYPCGLMGEEIPIEARIVSVADVYDALTSDRSYRKAYTFEKARQMVREMAGTALDPAVVEVFLAVVPDGPLPSSE
EFPECDSHRAGGKKPLNLVLPAATC

Sequences:

>Translated_345_residues
MAAEILFVDDNKLVLQALSDLLRSHGIAPLAASDAEEALELFKKHEIAVVVSDNQMPGMSGLEFLRELRNVSPDTVKVLI
SSHVDLPTALAAINSSEVYRCMMKPWKDEEIVEVVQEGLRRYRTIHDIRREDEAVLRSLAQTIELKDPSTKGHCDRVAVY
ALLVAEAMGIAKEMQRQIKYGSWLHDCGKIGVSEFILNGPGKLSEDEFETMKMHTDWGVDVAEKADLSQVARNIIHYHHE
KYDGSGYPCGLMGEEIPIEARIVSVADVYDALTSDRSYRKAYTFEKARQMVREMAGTALDPAVVEVFLAVVPDGPLPSSE
EFPECDSHRAGGKKPLNLVLPAATC
>Mature_344_residues
AAEILFVDDNKLVLQALSDLLRSHGIAPLAASDAEEALELFKKHEIAVVVSDNQMPGMSGLEFLRELRNVSPDTVKVLIS
SHVDLPTALAAINSSEVYRCMMKPWKDEEIVEVVQEGLRRYRTIHDIRREDEAVLRSLAQTIELKDPSTKGHCDRVAVYA
LLVAEAMGIAKEMQRQIKYGSWLHDCGKIGVSEFILNGPGKLSEDEFETMKMHTDWGVDVAEKADLSQVARNIIHYHHEK
YDGSGYPCGLMGEEIPIEARIVSVADVYDALTSDRSYRKAYTFEKARQMVREMAGTALDPAVVEVFLAVVPDGPLPSSEE
FPECDSHRAGGKKPLNLVLPAATC

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38279; Mature: 38148

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEILFVDDNKLVLQALSDLLRSHGIAPLAASDAEEALELFKKHEIAVVVSDNQMPGMS
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHH
GLEFLRELRNVSPDTVKVLISSHVDLPTALAAINSSEVYRCMMKPWKDEEIVEVVQEGLR
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
RYRTIHDIRREDEAVLRSLAQTIELKDPSTKGHCDRVAVYALLVAEAMGIAKEMQRQIKY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSWLHDCGKIGVSEFILNGPGKLSEDEFETMKMHTDWGVDVAEKADLSQVARNIIHYHHE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHH
KYDGSGYPCGLMGEEIPIEARIVSVADVYDALTSDRSYRKAYTFEKARQMVREMAGTALD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
PAVVEVFLAVVPDGPLPSSEEFPECDSHRAGGKKPLNLVLPAATC
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure 
AAEILFVDDNKLVLQALSDLLRSHGIAPLAASDAEEALELFKKHEIAVVVSDNQMPGMS
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHH
GLEFLRELRNVSPDTVKVLISSHVDLPTALAAINSSEVYRCMMKPWKDEEIVEVVQEGLR
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
RYRTIHDIRREDEAVLRSLAQTIELKDPSTKGHCDRVAVYALLVAEAMGIAKEMQRQIKY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSWLHDCGKIGVSEFILNGPGKLSEDEFETMKMHTDWGVDVAEKADLSQVARNIIHYHHE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHH
KYDGSGYPCGLMGEEIPIEARIVSVADVYDALTSDRSYRKAYTFEKARQMVREMAGTALD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
PAVVEVFLAVVPDGPLPSSEEFPECDSHRAGGKKPLNLVLPAATC
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]