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The map label for this gene is rimO

Identifier: 197117015

GI number: 197117015

Start: 738968

End: 740314

Strand: Reverse

Name: rimO

Synonym: Gbem_0618

Alternate gene names: 197117015

Gene position: 740314-738968 (Counterclockwise)

Preceding gene: 197117016

Following gene: 197117014

Centisome position: 16.04

GC content: 59.02

Gene sequence:

>1347_bases
TTGAGCGAGAAGACTAAGGAAAAAGTGAGCCTGGTGAGCCTGGGCTGCCCGAAGAACCTGGTGGATGCCGAGGTGATGCT
CGGCTACCTGTCGCAGGACGAGTACGAGGTTACCACCGACGAGACCCAGGCGGATATCATCGTGGTCAACACCTGCTCCT
TCATAAAAGAGGCGAAACAGGAGAGCATCGACACCATCCTGGACCTGGCCGACAGGAAGCATGACGCACGCTGCAAGCTG
CTCATCGTCACCGGCTGCCTCCCCCAGCGCTACCAGCAGGAACTCGCCAAGGAGCTCCCCGAGGTCGACATCTTCATCGG
TACCGGCGACTACCCGCGCATCGCGGAGATCATCGCCGAGAAGAAGCAGACCGAGGAGCAGCTCTGCTACACCGGGGATC
CCAACTTCGTCTACAACGACGAGGTCCCGCGGCTTCAGTCGTCGCCGCACTACACGGCCTATCTGAAGATAGCCGAGGGA
TGCTCCAACAACTGCTCTTACTGCGTCATCCCGTCTCTGCGCGGCGGCCACCGCTCGCGCCCGCTGGCGACGCTTCTGGC
CGAGGCGAAGGCGCTGGTCGAGGGAGGGGTCAAGGAGCTGAACCTGATCGCCCAGGACATCACCGCCTACGGCCGCGACC
TGGAGGGAAAACCGAGCCTGGAGCTTCTGATCAAGGAACTGGTCAAGATGGAGAAGCTCAAGTGGATCAGGCTTTTGTAC
GCCTATCCCGACGGGGTGACCGACTCGCTGATCCAGCTCATCAAGGAGGAGCCCAAGGTCTGCAAGTACCTCGACCTCCC
CATCCAGCACATCAGCGACCCGGTGCTCTCCAACATGAAACGCAGAAGCGGCGAGGCCGAGATCAGGACCCTGATCTCCA
AGCTGCGCCGCGAGATACCGGACATCGCCATCAGGACCTCGCTCATCGTCGGCTTCCCGGGCGAGACCACCGACGACTTC
AAGAAGCTCTTGCAGTTCGTCGAGCAGACCCGCTTCGATCGCCTCGGGGTCTTCTGCTACTCCAGGGAGGATGGGACCCC
TGCGGCGGAGATGCCGGACCAGGTGTCCGAGCGGATCAAGAGGGAGCGCCACAAGAAGCTGATGCGGACCCAGGCACGCG
TTTCCTTCAAGCACAATAGGACCTTGGTTGACAGTGAAGAGGACGTATTAGTTGAAGGTTACAGCGAAGAAACTGAGCTC
TTGCTCAAAGGTCGTTCCTCACGGCAAGCACCTGATGTCGATGGTCTGGTGTATATCACTGCAGGTAATGCCAACGTCGG
TGACATAGTGAGATTGAAGATTACTGATTCCTCTGATTACGACTTGATAGGTGAAATCGTAGACTAG

Upstream 100 bases:

>100_bases
ACGACCTGCAGGAGGTGATCCGGGTGCTTAAAGGGAAGGACCTCGACATCGATATGCAGTTCGTGAACTTCAGAAGCTAG
AAAAGCAAGAGAGGTTTTTT

Downstream 100 bases:

>100_bases
GCTTTCTACTTGACATGAACCCCTATTGCTGTATTATTGCGTCGCTTTGAGGGACACCAACAGTACGGAGTGTATTTTTA
CATGACAGAAAAACCGGAAG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSEKTKEKVSLVSLGCPKNLVDAEVMLGYLSQDEYEVTTDETQADIIVVNTCSFIKEAKQESIDTILDLADRKHDARCKL
LIVTGCLPQRYQQELAKELPEVDIFIGTGDYPRIAEIIAEKKQTEEQLCYTGDPNFVYNDEVPRLQSSPHYTAYLKIAEG
CSNNCSYCVIPSLRGGHRSRPLATLLAEAKALVEGGVKELNLIAQDITAYGRDLEGKPSLELLIKELVKMEKLKWIRLLY
AYPDGVTDSLIQLIKEEPKVCKYLDLPIQHISDPVLSNMKRRSGEAEIRTLISKLRREIPDIAIRTSLIVGFPGETTDDF
KKLLQFVEQTRFDRLGVFCYSREDGTPAAEMPDQVSERIKRERHKKLMRTQARVSFKHNRTLVDSEEDVLVEGYSEETEL
LLKGRSSRQAPDVDGLVYITAGNANVGDIVRLKITDSSDYDLIGEIVD

Sequences:

>Translated_448_residues
MSEKTKEKVSLVSLGCPKNLVDAEVMLGYLSQDEYEVTTDETQADIIVVNTCSFIKEAKQESIDTILDLADRKHDARCKL
LIVTGCLPQRYQQELAKELPEVDIFIGTGDYPRIAEIIAEKKQTEEQLCYTGDPNFVYNDEVPRLQSSPHYTAYLKIAEG
CSNNCSYCVIPSLRGGHRSRPLATLLAEAKALVEGGVKELNLIAQDITAYGRDLEGKPSLELLIKELVKMEKLKWIRLLY
AYPDGVTDSLIQLIKEEPKVCKYLDLPIQHISDPVLSNMKRRSGEAEIRTLISKLRREIPDIAIRTSLIVGFPGETTDDF
KKLLQFVEQTRFDRLGVFCYSREDGTPAAEMPDQVSERIKRERHKKLMRTQARVSFKHNRTLVDSEEDVLVEGYSEETEL
LLKGRSSRQAPDVDGLVYITAGNANVGDIVRLKITDSSDYDLIGEIVD
>Mature_447_residues
SEKTKEKVSLVSLGCPKNLVDAEVMLGYLSQDEYEVTTDETQADIIVVNTCSFIKEAKQESIDTILDLADRKHDARCKLL
IVTGCLPQRYQQELAKELPEVDIFIGTGDYPRIAEIIAEKKQTEEQLCYTGDPNFVYNDEVPRLQSSPHYTAYLKIAEGC
SNNCSYCVIPSLRGGHRSRPLATLLAEAKALVEGGVKELNLIAQDITAYGRDLEGKPSLELLIKELVKMEKLKWIRLLYA
YPDGVTDSLIQLIKEEPKVCKYLDLPIQHISDPVLSNMKRRSGEAEIRTLISKLRREIPDIAIRTSLIVGFPGETTDDFK
KLLQFVEQTRFDRLGVFCYSREDGTPAAEMPDQVSERIKRERHKKLMRTQARVSFKHNRTLVDSEEDVLVEGYSEETELL
LKGRSSRQAPDVDGLVYITAGNANVGDIVRLKITDSSDYDLIGEIVD

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=28.9583333333333, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=28.9583333333333, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI93277076, Length=450, Percent_Identity=28.2222222222222, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1787057, Length=458, Percent_Identity=37.7729257641921, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=30.561797752809, Blast_Score=205, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=25.4777070063694, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71996771, Length=300, Percent_Identity=32.6666666666667, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=27.1398747390397, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=26.9146608315098, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_GEOBB (B5ED60)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137442.1
- ProteinModelPortal:   B5ED60
- SMR:   B5ED60
- GeneID:   6783451
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0618
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK829158
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50553; Mature: 50422

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTKEKVSLVSLGCPKNLVDAEVMLGYLSQDEYEVTTDETQADIIVVNTCSFIKEAKQ
CCCHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECHHHHHHHHH
ESIDTILDLADRKHDARCKLLIVTGCLPQRYQQELAKELPEVDIFIGTGDYPRIAEIIAE
HHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
KKQTEEQLCYTGDPNFVYNDEVPRLQSSPHYTAYLKIAEGCSNNCSYCVIPSLRGGHRSR
HHHHHHCCEECCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCC
PLATLLAEAKALVEGGVKELNLIAQDITAYGRDLEGKPSLELLIKELVKMEKLKWIRLLY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHE
AYPDGVTDSLIQLIKEEPKVCKYLDLPIQHISDPVLSNMKRRSGEAEIRTLISKLRREIP
ECCCCHHHHHHHHHHHCCHHHHHHCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
DIAIRTSLIVGFPGETTDDFKKLLQFVEQTRFDRLGVFCYSREDGTPAAEMPDQVSERIK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHEEEEEECCCCCCHHCCCHHHHHHHH
RERHKKLMRTQARVSFKHNRTLVDSEEDVLVEGYSEETELLLKGRSSRQAPDVDGLVYIT
HHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCEEEEE
AGNANVGDIVRLKITDSSDYDLIGEIVD
ECCCCCCCEEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
SEKTKEKVSLVSLGCPKNLVDAEVMLGYLSQDEYEVTTDETQADIIVVNTCSFIKEAKQ
CCHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECHHHHHHHHH
ESIDTILDLADRKHDARCKLLIVTGCLPQRYQQELAKELPEVDIFIGTGDYPRIAEIIAE
HHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
KKQTEEQLCYTGDPNFVYNDEVPRLQSSPHYTAYLKIAEGCSNNCSYCVIPSLRGGHRSR
HHHHHHCCEECCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCC
PLATLLAEAKALVEGGVKELNLIAQDITAYGRDLEGKPSLELLIKELVKMEKLKWIRLLY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHE
AYPDGVTDSLIQLIKEEPKVCKYLDLPIQHISDPVLSNMKRRSGEAEIRTLISKLRREIP
ECCCCHHHHHHHHHHHCCHHHHHHCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCC
DIAIRTSLIVGFPGETTDDFKKLLQFVEQTRFDRLGVFCYSREDGTPAAEMPDQVSERIK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHEEEEEECCCCCCHHCCCHHHHHHHH
RERHKKLMRTQARVSFKHNRTLVDSEEDVLVEGYSEETELLLKGRSSRQAPDVDGLVYIT
HHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCEEEEE
AGNANVGDIVRLKITDSSDYDLIGEIVD
ECCCCCCCEEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA