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The map label for this gene is folK [H]

Identifier: 197117005

GI number: 197117005

Start: 729656

End: 730144

Strand: Direct

Name: folK [H]

Synonym: Gbem_0608

Alternate gene names: 197117005

Gene position: 729656-730144 (Clockwise)

Preceding gene: 197117002

Following gene: 197117006

Centisome position: 15.81

GC content: 64.83

Gene sequence:

>489_bases
GTGGAAGAGCGCGCCTACATAGGGTTGGGGAGCAACCTCGGCGACCGCGAGCTGAAGCTACTGATGGCCGTCGCCGAACT
GGGGAAGCTCCCCGGAACGCGGGTCACCGGCGTGTCCCAGTTCTACGAGACCGAGCCCGTGGGGGGCGTTCCCCAGGACA
ACTTCTACAACGCCGTGGCGCGCATTGAAACGACACTCTCTCCTTTCCTGCTCCTGGACGAGTTGAAGCGGGTGGAGACC
GAAGTCTTCCAGCGCAAGCCTTCAGAGCGCTGGGGCGCCCGCAGCATGGACCTGGACATCCTCCTCTACGGGGAGCTGGT
GCTCGACTCGGACCGGCTCACCATTCCGCACCCGCGCCTGGCGGAGCGGCGTTTCGCGCTGCAGCCGCTGGCCGACCTCG
CCCCCGACCTAGTGTATCCCGTCACAGGCAAAACCGTCTCGCAGCTTTTGGCTGCACTTACCGCCACCGAGAAGGTGGTC
AAGCTTTAG

Upstream 100 bases:

>100_bases
AAAACAGCACTGGCGCGGGGTCGCGCCGGGCCCGGCGCGCTTTTAGCTCATGAGCGGCCGGGGTTGAAAAAAATAAGGAA
TTGTGCTCTAATCCCACGCT

Downstream 100 bases:

>100_bases
GGGGAAGGTGTGAAGATACTGATCTGGGTGCTCCTTGGCTACCTCGCCTACCGGATGCTCAAGGGGAAGTCCGAGGCGAA
GATCGCCAAAAAGGAACCGG

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MEERAYIGLGSNLGDRELKLLMAVAELGKLPGTRVTGVSQFYETEPVGGVPQDNFYNAVARIETTLSPFLLLDELKRVET
EVFQRKPSERWGARSMDLDILLYGELVLDSDRLTIPHPRLAERRFALQPLADLAPDLVYPVTGKTVSQLLAALTATEKVV
KL

Sequences:

>Translated_162_residues
MEERAYIGLGSNLGDRELKLLMAVAELGKLPGTRVTGVSQFYETEPVGGVPQDNFYNAVARIETTLSPFLLLDELKRVET
EVFQRKPSERWGARSMDLDILLYGELVLDSDRLTIPHPRLAERRFALQPLADLAPDLVYPVTGKTVSQLLAALTATEKVV
KL
>Mature_162_residues
MEERAYIGLGSNLGDRELKLLMAVAELGKLPGTRVTGVSQFYETEPVGGVPQDNFYNAVARIETTLSPFLLLDELKRVET
EVFQRKPSERWGARSMDLDILLYGELVLDSDRLTIPHPRLAERRFALQPLADLAPDLVYPVTGKTVSQLLAALTATEKVV
KL

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=146, Percent_Identity=40.4109589041096, Blast_Score=119, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324073, Length=157, Percent_Identity=35.6687898089172, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18025; Mature: 18025

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERAYIGLGSNLGDRELKLLMAVAELGKLPGTRVTGVSQFYETEPVGGVPQDNFYNAVA
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
RIETTLSPFLLLDELKRVETEVFQRKPSERWGARSMDLDILLYGELVLDSDRLTIPHPRL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEEEEEECCCCCCCCCCHH
AERRFALQPLADLAPDLVYPVTGKTVSQLLAALTATEKVVKL
HHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEERAYIGLGSNLGDRELKLLMAVAELGKLPGTRVTGVSQFYETEPVGGVPQDNFYNAVA
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
RIETTLSPFLLLDELKRVETEVFQRKPSERWGARSMDLDILLYGELVLDSDRLTIPHPRL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEEEEEECCCCCCCCCCHH
AERRFALQPLADLAPDLVYPVTGKTVSQLLAALTATEKVVKL
HHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10080886 [H]