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The map label for this gene is gspG [H]

Identifier: 197116989

GI number: 197116989

Start: 710480

End: 710923

Strand: Reverse

Name: gspG [H]

Synonym: Gbem_0592

Alternate gene names: 197116989

Gene position: 710923-710480 (Counterclockwise)

Preceding gene: 197116990

Following gene: 308535176

Centisome position: 15.4

GC content: 61.26

Gene sequence:

>444_bases
ATGTTCAAGACACTGAAAGACAGCCGCGGCTTCACACTGATCGAACTGATGGTGGTCATCGTCATCCTGAGCATCCTGGC
CGTCATGGTCGGGCCGAAGATCATCGGCCGCAGCGACGACGCAAAGATCGCCGACGCGAAGGTGCAGATCCGCAACATCG
AGACGGCGCTCAAGCTATACAAGCTCGACAGCGGCAGCTACCCCACAACGGAGCAGGGGCTGCAGGCGCTGGTGACTAAG
CCGACGACCGGGAAGATCCCCAACAACTACAAAGCGGAAGGGTACCTGGAGAACAAGAACGTTCCCAAGGATCCCTGGGG
CGGCGACTACGTCTACCTCTCCCCCGGCGAGCACGGCGACTACGACCTCTCCTCCTTCGGCGCCGACGGCGCGCGCGGCG
GCGAAGGGAACAACGCCGACATCCAGAGCTGGAATATGAAGTAG

Upstream 100 bases:

>100_bases
GGTGCTGCTCCCCATCATGCAGCTCAATCAGCTGGTCAAATGAGCAACGGGATACCATCGCTTAAATACAATCCGACTTT
CTGATATTGGAGGTTTTGCA

Downstream 100 bases:

>100_bases
AGGCGGCGACAACGGTATGGCAACGCCCTCACCCCGACCCTCTCCCAAGGGGAGAGGGAGGATAGACGGGAGCATCGGGT
CTTTGGCGGATAAGAGTACG

Product: type II secretion system pseudopilin GspG

Products: NA

Alternate protein names: Cholera toxin secretion protein epsG [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MFKTLKDSRGFTLIELMVVIVILSILAVMVGPKIIGRSDDAKIADAKVQIRNIETALKLYKLDSGSYPTTEQGLQALVTK
PTTGKIPNNYKAEGYLENKNVPKDPWGGDYVYLSPGEHGDYDLSSFGADGARGGEGNNADIQSWNMK

Sequences:

>Translated_147_residues
MFKTLKDSRGFTLIELMVVIVILSILAVMVGPKIIGRSDDAKIADAKVQIRNIETALKLYKLDSGSYPTTEQGLQALVTK
PTTGKIPNNYKAEGYLENKNVPKDPWGGDYVYLSPGEHGDYDLSSFGADGARGGEGNNADIQSWNMK
>Mature_147_residues
MFKTLKDSRGFTLIELMVVIVILSILAVMVGPKIIGRSDDAKIADAKVQIRNIETALKLYKLDSGSYPTTEQGLQALVTK
PTTGKIPNNYKAEGYLENKNVPKDPWGGDYVYLSPGEHGDYDLSSFGADGARGGEGNNADIQSWNMK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP G family [H]

Homologues:

Organism=Escherichia coli, GI1789725, Length=139, Percent_Identity=49.6402877697842, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000983
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR013545
- InterPro:   IPR010054 [H]

Pfam domain/function: PF08334 GSPII_G; PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 16026; Mature: 16026

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKTLKDSRGFTLIELMVVIVILSILAVMVGPKIIGRSDDAKIADAKVQIRNIETALKLY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEECCEEEEHHHHHHHHEE
KLDSGSYPTTEQGLQALVTKPTTGKIPNNYKAEGYLENKNVPKDPWGGDYVYLSPGEHGD
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCC
YDLSSFGADGARGGEGNNADIQSWNMK
CCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKTLKDSRGFTLIELMVVIVILSILAVMVGPKIIGRSDDAKIADAKVQIRNIETALKLY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEECCEEEEHHHHHHHHEE
KLDSGSYPTTEQGLQALVTKPTTGKIPNNYKAEGYLENKNVPKDPWGGDYVYLSPGEHGD
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCC
YDLSSFGADGARGGEGNNADIQSWNMK
CCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]