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The map label for this gene is mceE [H]

Identifier: 197116956

GI number: 197116956

Start: 671567

End: 671980

Strand: Reverse

Name: mceE [H]

Synonym: Gbem_0558

Alternate gene names: 197116956

Gene position: 671980-671567 (Counterclockwise)

Preceding gene: 197116958

Following gene: 197116955

Centisome position: 14.56

GC content: 57.0

Gene sequence:

>414_bases
ATGTTATCTAAGATAAATCATGTCGGCGTTGCAGTCGCTTCTCTTGAGGAAGCACTTCCTTTTTATCGCGATTCACTGGG
CATGACTTTCTCTGGTACAGAAGAGGTCCCAAGTCAACTAGTACGGGTAGCTTTTCTGTCAGTAGGCGAATCGAAGATCG
AACTTCTGGAGCCGACCTCCCCCGAGAGTCCGGTGACCAAGTTCCTGGAGAAGAACGGCCCGGGGGTGCACCACGTGGCT
TACGGGGTTGACGATATCGAGGCGGCTATCGCAGCGCTGGTGGCCAAAGGGACCCGCATGATCGACGCCGTACCCCGCAA
CGGCGCCCACGGCGCGCGTATCGCCTTCATCCATCCCAAGAGCAGCGGCGGCGTGCTGACGGAACTGTGTGAAGTGCACG
ACCACAGTCATTGA

Upstream 100 bases:

>100_bases
ACAACTCTGACAAATTTTGCTCAGCTACTTGCAATTTGATTTTTTCCAGTTATTATCTACTTCCGTCAGTCTATGACCGA
CGATCTGTAAAGGAATATTA

Downstream 100 bases:

>100_bases
GACATTCAAATTTCGGAATCTAATGAGTCTGGATTTTTTTATTGACTTTTGTTTGAATAATAATATAGTGGCGTCAGTTT
TCTATAACAGTGGTTTGCTC

Product: methylmalonyl-CoA epimerase

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MLSKINHVGVAVASLEEALPFYRDSLGMTFSGTEEVPSQLVRVAFLSVGESKIELLEPTSPESPVTKFLEKNGPGVHHVA
YGVDDIEAAIAALVAKGTRMIDAVPRNGAHGARIAFIHPKSSGGVLTELCEVHDHSH

Sequences:

>Translated_137_residues
MLSKINHVGVAVASLEEALPFYRDSLGMTFSGTEEVPSQLVRVAFLSVGESKIELLEPTSPESPVTKFLEKNGPGVHHVA
YGVDDIEAAIAALVAKGTRMIDAVPRNGAHGARIAFIHPKSSGGVLTELCEVHDHSH
>Mature_137_residues
MLSKINHVGVAVASLEEALPFYRDSLGMTFSGTEEVPSQLVRVAFLSVGESKIELLEPTSPESPVTKFLEKNGPGVHHVA
YGVDDIEAAIAALVAKGTRMIDAVPRNGAHGARIAFIHPKSSGGVLTELCEVHDHSH

Specific function: Unknown

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI188035928, Length=129, Percent_Identity=42.6356589147287, Blast_Score=105, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17506387, Length=130, Percent_Identity=41.5384615384615, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR018146
- InterPro:   IPR017515 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14565; Mature: 14565

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKINHVGVAVASLEEALPFYRDSLGMTFSGTEEVPSQLVRVAFLSVGESKIELLEPTS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
PESPVTKFLEKNGPGVHHVAYGVDDIEAAIAALVAKGTRMIDAVPRNGAHGARIAFIHPK
CCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCHHEECCCCCCCCCCEEEEEECC
SSGGVLTELCEVHDHSH
CCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLSKINHVGVAVASLEEALPFYRDSLGMTFSGTEEVPSQLVRVAFLSVGESKIELLEPTS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
PESPVTKFLEKNGPGVHHVAYGVDDIEAAIAALVAKGTRMIDAVPRNGAHGARIAFIHPK
CCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHCCCHHEECCCCCCCCCCEEEEEECC
SSGGVLTELCEVHDHSH
CCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]