Click here to switch to the map view.

The map label for this gene is pncA [C]

Identifier: 197116906

GI number: 197116906

Start: 616644

End: 617297

Strand: Direct

Name: pncA [C]

Synonym: Gbem_0508

Alternate gene names: 197116906

Gene position: 616644-617297 (Clockwise)

Preceding gene: 308535168

Following gene: 197116907

Centisome position: 13.36

GC content: 52.75

Gene sequence:

>654_bases
GTGAAGCGATCTAAAAAAATAATGTTCTGCTCTGCTGGTTCTGTTCTTCTTCTGGTGCTTGTGCTATTCGCTGCGATCTA
CTGGAGTATGCGCCCCACAACGGGAACTCCAATCAACAAATATGCCACTCCACGCGCTGCGCTTCTTATCGTTGACATCC
AAGAAGACTACACTGGCCCGCAAGCAAAGAAACGCTACAGGGACGGTGATCGCATCGTCACTGTCTCTAATGCATTACTG
GCACAGGCCCAAGCAAACGGAGCCGTTGTCGTGTTTGTAAAGAACGTGATCGATAACCCGATCATGTCTGCTCTCATGGG
CGGAATCAACGCCCCAGATTCACCCGGTGCGGAAATGGACCGCCGCCTCATCGAAATTCCCGCGGCGGTTACTTTCCAGA
AGAGCCGGCCGGATGCGTTTTCCACTCCAGAGCTTGATGCCTATCTTAGAGAAAAGCAGGTAGACCAAATATTTATCACC
GGCCTGGACGGCGCCTACTGTGTGAGCGCCACTGCTCGTGGCGCTCTCAATCGCGGCTACAAAGTGACGCTGTTTCAGGA
CGGCGTAGCGACCGAAAGCAGCAAAAACATAGAGGAGTTGGCCAAGAGTTGGCGTCAAGCCGGAGCTCAAGTTAAGACGG
GCTCCGAGATTTAA

Upstream 100 bases:

>100_bases
GTTAGGTAAGAGGCAATATTCCAGGAGGTTCTGAAGGAATGCAAGCGCGCCTCAAGCTCATTCCGCCTCAACTGCGACTG
AATAGGGGTGGGGAGGTGTT

Downstream 100 bases:

>100_bases
TCCGACGTCCAGCCCGAGCGGTGTTATTCCCTGGTTGCACTATTAAAGAGGCGAAATGATAGCCATAACCTACAATGACC
GATTCTTTAAGGCGGTAAGC

Product: cysteine hydrolase family protein

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: Isochorismatase Hydrolase; Cysteine Hydrolase Family Protein

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKRSKKIMFCSAGSVLLLVLVLFAAIYWSMRPTTGTPINKYATPRAALLIVDIQEDYTGPQAKKRYRDGDRIVTVSNALL
AQAQANGAVVVFVKNVIDNPIMSALMGGINAPDSPGAEMDRRLIEIPAAVTFQKSRPDAFSTPELDAYLREKQVDQIFIT
GLDGAYCVSATARGALNRGYKVTLFQDGVATESSKNIEELAKSWRQAGAQVKTGSEI

Sequences:

>Translated_217_residues
MKRSKKIMFCSAGSVLLLVLVLFAAIYWSMRPTTGTPINKYATPRAALLIVDIQEDYTGPQAKKRYRDGDRIVTVSNALL
AQAQANGAVVVFVKNVIDNPIMSALMGGINAPDSPGAEMDRRLIEIPAAVTFQKSRPDAFSTPELDAYLREKQVDQIFIT
GLDGAYCVSATARGALNRGYKVTLFQDGVATESSKNIEELAKSWRQAGAQVKTGSEI
>Mature_217_residues
MKRSKKIMFCSAGSVLLLVLVLFAAIYWSMRPTTGTPINKYATPRAALLIVDIQEDYTGPQAKKRYRDGDRIVTVSNALL
AQAQANGAVVVFVKNVIDNPIMSALMGGINAPDSPGAEMDRRLIEIPAAVTFQKSRPDAFSTPELDAYLREKQVDQIFIT
GLDGAYCVSATARGALNRGYKVTLFQDGVATESSKNIEELAKSWRQAGAQVKTGSEI

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.- [C]

Molecular weight: Translated: 23576; Mature: 23576

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSKKIMFCSAGSVLLLVLVLFAAIYWSMRPTTGTPINKYATPRAALLIVDIQEDYTGP
CCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCH
QAKKRYRDGDRIVTVSNALLAQAQANGAVVVFVKNVIDNPIMSALMGGINAPDSPGAEMD
HHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHCCCCCCCCCCHHHH
RRLIEIPAAVTFQKSRPDAFSTPELDAYLREKQVDQIFITGLDGAYCVSATARGALNRGY
HHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHCCCE
KVTLFQDGVATESSKNIEELAKSWRQAGAQVKTGSEI
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKRSKKIMFCSAGSVLLLVLVLFAAIYWSMRPTTGTPINKYATPRAALLIVDIQEDYTGP
CCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCH
QAKKRYRDGDRIVTVSNALLAQAQANGAVVVFVKNVIDNPIMSALMGGINAPDSPGAEMD
HHHHHHCCCCEEEEEHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHCCCCCCCCCCHHHH
RRLIEIPAAVTFQKSRPDAFSTPELDAYLREKQVDQIFITGLDGAYCVSATARGALNRGY
HHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHCCCE
KVTLFQDGVATESSKNIEELAKSWRQAGAQVKTGSEI
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA