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The map label for this gene is murD

Identifier: 197116887

GI number: 197116887

Start: 590464

End: 591822

Strand: Direct

Name: murD

Synonym: Gbem_0489

Alternate gene names: 197116887

Gene position: 590464-591822 (Clockwise)

Preceding gene: 197116886

Following gene: 197116888

Centisome position: 12.79

GC content: 63.5

Gene sequence:

>1359_bases
ATGGAATTAAAGGACAAGAAAATACTGGTGGTGGGACTGGCGAAGACCGGCGTGGCGGTGACCCGCTTCCTGGCGCAAGC
CGGTGCCTTCGTCACCGTTACCGACATGCGGGAGGAGGAGGCGCTCTCCGACGTTCTGGCCGAGCTCTCCGACCTGGATA
TTACCTACGAACTCGGGCGCCACGTCCCCTACAGCTTCCTTATGGCGGATCTGATCGTGGTCTCACCCGGCGTCCCCATG
GACATAAAGCCGCTGGAGATGGCCCGCTCGCAGAAGCGGCGCGTGGTGAGCGAGGTGGAACTCGCCTCCTGGTTCATCAA
GGCCCCCATGGTCGCCATCACCGGGACCAACGGCAAGACCACGACGACGACCCTTACCGGCGAGATCTTCAAGGCCTGCG
GCTTCGAGACCTTCGTGGGGGGGAACATCGGCAATCCCCTGATCGAGCTGGCCGAAAGCGGCCAGGAGGTATCAAGGGTG
GTGGTGGAACTCTCCTCCTTCCAGCTGGAAGGGGTGGAAAGCTTCCGGCCGGACGTGGCGGTGCTTTTGAACATCACCGA
GGACCACCTGGACCGCTACCACAGCTTCCAGGAGTACATCGACGCGAAGCTCCGCATCTTCGAGAACCAGACCGCGGACG
ACTTCGCCGTCCTCAACATCGACGACCCGCTGGTCGCTGCCTGCGCCTCCAAGCTCAAGGCGCAGCTCTTCCCCATGAGC
CGCCTGCACGAGCTGGAAGAAGGGATCAGCTACCGCGACGGCTTCATCACCTTCTCCCACAAGGGGAAAGTGCTCCGCTT
CGGCACCGAGGGGTTCAAGCTGAAGGGGGTGCACAACCTGGACAACATCATGGCGAGCCTTGCCTCCACCCTCCTCATGC
GCTGCGACGGCGACTGCGCCTACGAGGCGGTGAAGAACTTCAAGGGGCTGCCGCACCGGATGGAACTGGTGGAAGAGATC
GACGGCGTCGCCTACTACGAGGACAGCAAAGGGACCAACGTCGGGAGCGTGGTGAAGTCGCTGGAGAGCTTCGACTCCGG
CATCACCCTCATCGCCGGCGGCAAGGACAAGGGAGGCTCCTATGAGCCCCTGGCCCCCCTGGTCGAGAGCCGAGTGAGCC
ACCTGGTGCTGATCGGCGAGGCGAAGGCACGCATGAACGAGGCGCTAGGCTCCTTGACCGATACCCATCTCGCCGAAACC
CTCGAGGAGGCGGTGGAGATCTCGCGCAGGCTGACCAAGCCGGGCGGGGTGGTGCTTTTCTCGCCGGCCTGCTCCAGCTT
CGACATGTTCAAGAACTACGAGGAGCGCGCCCAGCGCTTCAAGGCCGCGGTGCGCGCCGGCAAGAAGGGGGAGGCGTGA

Upstream 100 bases:

>100_bases
CACGACCGGCGCCCATGGCAGCTTCACCCACCCCCCGGCCCCCTCCCGTCAAGGGAGGGGGGGATAGCCGGGCGGCAGAT
CGATAACAAAAGGGTCCATC

Downstream 100 bases:

>100_bases
GGAAACTGGAGGGGTACGACATGATCGTGCTGCTGATGGCGGTGATCCTCACCTGTTTCGGTGTGGTCATGGTCTATTCG
GCGTCCTCCGTGATGGCGGC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MELKDKKILVVGLAKTGVAVTRFLAQAGAFVTVTDMREEEALSDVLAELSDLDITYELGRHVPYSFLMADLIVVSPGVPM
DIKPLEMARSQKRRVVSEVELASWFIKAPMVAITGTNGKTTTTTLTGEIFKACGFETFVGGNIGNPLIELAESGQEVSRV
VVELSSFQLEGVESFRPDVAVLLNITEDHLDRYHSFQEYIDAKLRIFENQTADDFAVLNIDDPLVAACASKLKAQLFPMS
RLHELEEGISYRDGFITFSHKGKVLRFGTEGFKLKGVHNLDNIMASLASTLLMRCDGDCAYEAVKNFKGLPHRMELVEEI
DGVAYYEDSKGTNVGSVVKSLESFDSGITLIAGGKDKGGSYEPLAPLVESRVSHLVLIGEAKARMNEALGSLTDTHLAET
LEEAVEISRRLTKPGGVVLFSPACSSFDMFKNYEERAQRFKAAVRAGKKGEA

Sequences:

>Translated_452_residues
MELKDKKILVVGLAKTGVAVTRFLAQAGAFVTVTDMREEEALSDVLAELSDLDITYELGRHVPYSFLMADLIVVSPGVPM
DIKPLEMARSQKRRVVSEVELASWFIKAPMVAITGTNGKTTTTTLTGEIFKACGFETFVGGNIGNPLIELAESGQEVSRV
VVELSSFQLEGVESFRPDVAVLLNITEDHLDRYHSFQEYIDAKLRIFENQTADDFAVLNIDDPLVAACASKLKAQLFPMS
RLHELEEGISYRDGFITFSHKGKVLRFGTEGFKLKGVHNLDNIMASLASTLLMRCDGDCAYEAVKNFKGLPHRMELVEEI
DGVAYYEDSKGTNVGSVVKSLESFDSGITLIAGGKDKGGSYEPLAPLVESRVSHLVLIGEAKARMNEALGSLTDTHLAET
LEEAVEISRRLTKPGGVVLFSPACSSFDMFKNYEERAQRFKAAVRAGKKGEA
>Mature_452_residues
MELKDKKILVVGLAKTGVAVTRFLAQAGAFVTVTDMREEEALSDVLAELSDLDITYELGRHVPYSFLMADLIVVSPGVPM
DIKPLEMARSQKRRVVSEVELASWFIKAPMVAITGTNGKTTTTTLTGEIFKACGFETFVGGNIGNPLIELAESGQEVSRV
VVELSSFQLEGVESFRPDVAVLLNITEDHLDRYHSFQEYIDAKLRIFENQTADDFAVLNIDDPLVAACASKLKAQLFPMS
RLHELEEGISYRDGFITFSHKGKVLRFGTEGFKLKGVHNLDNIMASLASTLLMRCDGDCAYEAVKNFKGLPHRMELVEEI
DGVAYYEDSKGTNVGSVVKSLESFDSGITLIAGGKDKGGSYEPLAPLVESRVSHLVLIGEAKARMNEALGSLTDTHLAET
LEEAVEISRRLTKPGGVVLFSPACSSFDMFKNYEERAQRFKAAVRAGKKGEA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=446, Percent_Identity=35.4260089686099, Blast_Score=221, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_GEOBB (B5EBP9)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137314.1
- ProteinModelPortal:   B5EBP9
- GeneID:   6783318
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0489
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK829090
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49480; Mature: 49480

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKDKKILVVGLAKTGVAVTRFLAQAGAFVTVTDMREEEALSDVLAELSDLDITYELGR
CCCCCCEEEEEEECHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCEEHHHCC
HVPYSFLMADLIVVSPGVPMDIKPLEMARSQKRRVVSEVELASWFIKAPMVAITGTNGKT
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TTTTLTGEIFKACGFETFVGGNIGNPLIELAESGQEVSRVVVELSSFQLEGVESFRPDVA
EEEEEHHHHHHHCCCHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCCCEE
VLLNITEDHLDRYHSFQEYIDAKLRIFENQTADDFAVLNIDDPLVAACASKLKAQLFPMS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHH
RLHELEEGISYRDGFITFSHKGKVLRFGTEGFKLKGVHNLDNIMASLASTLLMRCDGDCA
HHHHHHHCCCCCCCEEEEECCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHH
YEAVKNFKGLPHRMELVEEIDGVAYYEDSKGTNVGSVVKSLESFDSGITLIAGGKDKGGS
HHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCC
YEPLAPLVESRVSHLVLIGEAKARMNEALGSLTDTHLAETLEEAVEISRRLTKPGGVVLF
CCCHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SPACSSFDMFKNYEERAQRFKAAVRAGKKGEA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MELKDKKILVVGLAKTGVAVTRFLAQAGAFVTVTDMREEEALSDVLAELSDLDITYELGR
CCCCCCEEEEEEECHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCEEHHHCC
HVPYSFLMADLIVVSPGVPMDIKPLEMARSQKRRVVSEVELASWFIKAPMVAITGTNGKT
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TTTTLTGEIFKACGFETFVGGNIGNPLIELAESGQEVSRVVVELSSFQLEGVESFRPDVA
EEEEEHHHHHHHCCCHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCCCEE
VLLNITEDHLDRYHSFQEYIDAKLRIFENQTADDFAVLNIDDPLVAACASKLKAQLFPMS
EEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHH
RLHELEEGISYRDGFITFSHKGKVLRFGTEGFKLKGVHNLDNIMASLASTLLMRCDGDCA
HHHHHHHCCCCCCCEEEEECCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHH
YEAVKNFKGLPHRMELVEEIDGVAYYEDSKGTNVGSVVKSLESFDSGITLIAGGKDKGGS
HHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCC
YEPLAPLVESRVSHLVLIGEAKARMNEALGSLTDTHLAETLEEAVEISRRLTKPGGVVLF
CCCHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SPACSSFDMFKNYEERAQRFKAAVRAGKKGEA
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA