| Definition | Geobacter bemidjiensis Bem chromosome, complete genome. |
|---|---|
| Accession | NC_011146 |
| Length | 4,615,150 |
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The map label for this gene is anfA [H]
Identifier: 197116830
GI number: 197116830
Start: 511796
End: 514378
Strand: Reverse
Name: anfA [H]
Synonym: Gbem_0432
Alternate gene names: 197116830
Gene position: 514378-511796 (Counterclockwise)
Preceding gene: 197116831
Following gene: 197116829
Centisome position: 11.15
GC content: 66.55
Gene sequence:
>2583_bases GTGGCGGCAATAAGCTCTGAAACCATTTCCGGCATCCGCCTTTTCAGCACACTCAACCGGGAGCAGCTGGACCGCATCGC CCGACACCTGCAGCTGCGCGAGTTCGCGCCGGGTCAGATCATCCTTTCCAGGAACGAGCCGGCGCTGGAACTCTACGTCA TCCTCGCCGGAAGGATCAGGGTGGAACTCCTGGACGAAGGGGGGCAGGTGCTGACCCTCACCGAACTCGGCATCGGCAAC GTCATCGGGGAGCGCGCCATCCTGACCGACGAGAAGCGCTCCGCCGACGTGAGGGCGATCACCGAGGTCCAGGCGGCACG GCTCTCCCGGGAGGATTTCGAAGAGCTTCTGGACCAAATCCCCGCGCTCTACGCCAACCTGAGCCGGATCTTCGCGGCGC AGCTGGGTAGCTGGGCGCACCGCCACCAGCGCGAGGAAAGCGAACACCGCGAGGTGATCACCAACATCATCGGCTGGCAG TTGCTTCCCGAGTTCGGCCAGTTCCCCGGCGCCTCGCACTGGGTGCGCCTTTTAAACCAACGCCTGCAGCAGCTGGGCGG CACCCGCAGCCACGTCCTCATCCTGGGCGAGCCCGGCACCTGGAAGGATCTCGCCGCCCGGCTGATCCACTTCCACAGCG AAGCCGACCGCCCCGTCCTTTTCCTGGACTGCGCCTCCCCCCCGCCGGTACTGGAAGAGGAAAACGAGCCAGCCGAGGGC GCCTCCCAAAGGGGTGTGCTGCTAGGTCTCGCCCAGGAGGCGGCGCTTTTCGGCCATGCTTCGCAGGGGGCGGTCTATGC GCGGCGGGTGAGAAGGGGGATGATCGAGCTTGCCGCAGGGGGCGACATGATCCTGCGCAACGTCGACTGCCTGGCCCTTG CGGTGCAGGAGGAGCTGGTCGATTTCCTCGACACCGGGCACTTCACCAGAAGGGGCGAGACCAAACTACGCAGCGCCCGG GTGAGGATCATAGCCACCAGCGGCAAGGCCCTGGAGCCCCTGATCGACAGCGGCAAGTTCAACGGAGAGCTGTACCGAAA GCTCTGCGGCGAAACCGTGGAGTTAGCCCCGCTGCGCGAGCGCAAGAAGGACATCCCCGTCATCGCGAAGAGCCTCCTCG CCTCTCTCAACGCCAAGCACAACCGCAACGTGCGCCGGCTCTCCCAAGACGCCCTGAACCGCCTGGTAGACCACGACTGG CCCCTCAACGCCACCGAACTGTACCAGGTCGTGAGCCGCGCCGTGGTGGTCTGCAGCGACACGGAGATTCAGCCCGAGCA CATCTCCCTCCAGGGGCACCCCTTCGAGGACGGCCGCTTCAACCTCTTGACGCTTCCCTCCCTGGAGCGGCTCGCCTGGA ACCCGCGTTTCCCGAAGGTGCTGCGCTGGCTGACCGTACCGGCGTTCCTGCTGATCACCCTGTACACCCTGCTCGGCCCC GCTTCGGACAACGCGGCGAACCTTGCGGCCTGGACCCTGGGATGGCCCGCGCTGATACTCACCGCTTTCCTCTTCGCCCG CGGCTGGTGCAGCTTCTGTCCGATGGAAGCGATCGGCGAACGGCTTGGGGTCACCAGCCGTGTGGTGCGCGACCCGGCAC CGTGGCTGCGCAGTTTCGGGCCGTCGCTCAGTTTCGCCGCACTGGTGCTGATCCTCCTGATGGAGCAGGCGACCGGGATG TTCTCCCACGCAGCGGCCACGGGGCTGTTGCTGAGCGGCATGCTGACGGCGACGGTGAGCGCCGACCTGGTGATCGGCCG TCGCGGCTGGTGCAAGTTCCTCTGCCCGCTGGGGCGCATCGTGAGCCTCGTATCGCGCATCTCGCCGCTGGAGATGCACA GCAACCACAACGTCTGCCTGAGCAGGTGCCGGGTGGACGACTGCATCAAGGAGAAGGCCTGCCCCATGGGGCTGCACCCC TCCGGCGTCGACAGCTCCGACCACTGCGTCCTCTGTCTCAACTGCGTCCGCAACTGTCCGCACCACTCGATGCAGCTCGA CCTGAGGAACCCGACCTGCGGCGTGTTCAACAAGGCCCGGCGCGGCTTCAGGGAGGCGTTTTTCAGCGTCACCCTGCTGG GCGCGGTGATCGCCGCCAAGGGGACCCCGCTTTTGGCCGGGCGGCAACCGGAACTCTTCCCGCGCACCCTCTGGACCCTG GAGGACTACCTGCTGGCGCTTTGCCTCGTCGCCGGGTTCACCTTCCTTGCCCTCATCGCTTCGGTGGCTGTGCGCGGCGC CCGCTGGCGTTCCGTCTTCACCAGCAGCGGGCTCGCCTACCTCCCTCTCGCCGCCGCGGGTCTATTCCTGATCTTCTTCC GTCCGCTGGTGGAGGGGGGCGCCAGGCTGGTCCCGCTCGCCGTTTCGGCCCTGGGAGCGGAAAATTTCCTCGATGCCACG GCGCTCACCCCGGAACTTGGGACGCTGCGCCTGCTCATCTACCCGATTATCCTTTTGGCGGCGCTCTTTTCCTGGGTGGT GCTGGCCCGGCTGCAGCGCCTGGACGGGCTCCCGCCGGCGGCGCTGCTCCGGCACCGGCTGCTCATCCTCGGCGCCACCG CGATACTGATAAAAATCCTTTAG
Upstream 100 bases:
>100_bases CCCCCTCCCCCCCCTGCACGGTATACACCCGGTATACACATTTGAAGCTACAGCGGCTTTTGCGCTACCATCTGCTTCCC TTTCAATCTTAGGAGAACAT
Downstream 100 bases:
>100_bases TTGACATTCCGCGAGGGTCGGTATAAACAGGACGGGTTCCTCGTTACGATGAGGCGTTTGCTCAAACACAGGCTACTGCC CGGAAACGTCGAAAGACGCC
Product: cyclic nucleotide-binding sigma-54-dependent transcriptional regulator
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 860; Mature: 859
Protein sequence:
>860_residues MAAISSETISGIRLFSTLNREQLDRIARHLQLREFAPGQIILSRNEPALELYVILAGRIRVELLDEGGQVLTLTELGIGN VIGERAILTDEKRSADVRAITEVQAARLSREDFEELLDQIPALYANLSRIFAAQLGSWAHRHQREESEHREVITNIIGWQ LLPEFGQFPGASHWVRLLNQRLQQLGGTRSHVLILGEPGTWKDLAARLIHFHSEADRPVLFLDCASPPPVLEEENEPAEG ASQRGVLLGLAQEAALFGHASQGAVYARRVRRGMIELAAGGDMILRNVDCLALAVQEELVDFLDTGHFTRRGETKLRSAR VRIIATSGKALEPLIDSGKFNGELYRKLCGETVELAPLRERKKDIPVIAKSLLASLNAKHNRNVRRLSQDALNRLVDHDW PLNATELYQVVSRAVVVCSDTEIQPEHISLQGHPFEDGRFNLLTLPSLERLAWNPRFPKVLRWLTVPAFLLITLYTLLGP ASDNAANLAAWTLGWPALILTAFLFARGWCSFCPMEAIGERLGVTSRVVRDPAPWLRSFGPSLSFAALVLILLMEQATGM FSHAAATGLLLSGMLTATVSADLVIGRRGWCKFLCPLGRIVSLVSRISPLEMHSNHNVCLSRCRVDDCIKEKACPMGLHP SGVDSSDHCVLCLNCVRNCPHHSMQLDLRNPTCGVFNKARRGFREAFFSVTLLGAVIAAKGTPLLAGRQPELFPRTLWTL EDYLLALCLVAGFTFLALIASVAVRGARWRSVFTSSGLAYLPLAAAGLFLIFFRPLVEGGARLVPLAVSALGAENFLDAT ALTPELGTLRLLIYPIILLAALFSWVVLARLQRLDGLPPAALLRHRLLILGATAILIKIL
Sequences:
>Translated_860_residues MAAISSETISGIRLFSTLNREQLDRIARHLQLREFAPGQIILSRNEPALELYVILAGRIRVELLDEGGQVLTLTELGIGN VIGERAILTDEKRSADVRAITEVQAARLSREDFEELLDQIPALYANLSRIFAAQLGSWAHRHQREESEHREVITNIIGWQ LLPEFGQFPGASHWVRLLNQRLQQLGGTRSHVLILGEPGTWKDLAARLIHFHSEADRPVLFLDCASPPPVLEEENEPAEG ASQRGVLLGLAQEAALFGHASQGAVYARRVRRGMIELAAGGDMILRNVDCLALAVQEELVDFLDTGHFTRRGETKLRSAR VRIIATSGKALEPLIDSGKFNGELYRKLCGETVELAPLRERKKDIPVIAKSLLASLNAKHNRNVRRLSQDALNRLVDHDW PLNATELYQVVSRAVVVCSDTEIQPEHISLQGHPFEDGRFNLLTLPSLERLAWNPRFPKVLRWLTVPAFLLITLYTLLGP ASDNAANLAAWTLGWPALILTAFLFARGWCSFCPMEAIGERLGVTSRVVRDPAPWLRSFGPSLSFAALVLILLMEQATGM FSHAAATGLLLSGMLTATVSADLVIGRRGWCKFLCPLGRIVSLVSRISPLEMHSNHNVCLSRCRVDDCIKEKACPMGLHP SGVDSSDHCVLCLNCVRNCPHHSMQLDLRNPTCGVFNKARRGFREAFFSVTLLGAVIAAKGTPLLAGRQPELFPRTLWTL EDYLLALCLVAGFTFLALIASVAVRGARWRSVFTSSGLAYLPLAAAGLFLIFFRPLVEGGARLVPLAVSALGAENFLDAT ALTPELGTLRLLIYPIILLAALFSWVVLARLQRLDGLPPAALLRHRLLILGATAILIKIL >Mature_859_residues AAISSETISGIRLFSTLNREQLDRIARHLQLREFAPGQIILSRNEPALELYVILAGRIRVELLDEGGQVLTLTELGIGNV IGERAILTDEKRSADVRAITEVQAARLSREDFEELLDQIPALYANLSRIFAAQLGSWAHRHQREESEHREVITNIIGWQL LPEFGQFPGASHWVRLLNQRLQQLGGTRSHVLILGEPGTWKDLAARLIHFHSEADRPVLFLDCASPPPVLEEENEPAEGA SQRGVLLGLAQEAALFGHASQGAVYARRVRRGMIELAAGGDMILRNVDCLALAVQEELVDFLDTGHFTRRGETKLRSARV RIIATSGKALEPLIDSGKFNGELYRKLCGETVELAPLRERKKDIPVIAKSLLASLNAKHNRNVRRLSQDALNRLVDHDWP LNATELYQVVSRAVVVCSDTEIQPEHISLQGHPFEDGRFNLLTLPSLERLAWNPRFPKVLRWLTVPAFLLITLYTLLGPA SDNAANLAAWTLGWPALILTAFLFARGWCSFCPMEAIGERLGVTSRVVRDPAPWLRSFGPSLSFAALVLILLMEQATGMF SHAAATGLLLSGMLTATVSADLVIGRRGWCKFLCPLGRIVSLVSRISPLEMHSNHNVCLSRCRVDDCIKEKACPMGLHPS GVDSSDHCVLCLNCVRNCPHHSMQLDLRNPTCGVFNKARRGFREAFFSVTLLGAVIAAKGTPLLAGRQPELFPRTLWTLE DYLLALCLVAGFTFLALIASVAVRGARWRSVFTSSGLAYLPLAAAGLFLIFFRPLVEGGARLVPLAVSALGAENFLDATA LTPELGTLRLLIYPIILLAALFSWVVLARLQRLDGLPPAALLRHRLLILGATAILIKIL
Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]
COG id: COG3604
COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 sigma-54 factor interaction domain [H]
Homologues:
Organism=Escherichia coli, GI1789087, Length=262, Percent_Identity=30.9160305343511, Blast_Score=119, Evalue=1e-27, Organism=Escherichia coli, GI87082117, Length=240, Percent_Identity=30.8333333333333, Blast_Score=114, Evalue=2e-26, Organism=Escherichia coli, GI1788550, Length=233, Percent_Identity=30.4721030042918, Blast_Score=108, Evalue=1e-24, Organism=Escherichia coli, GI1790437, Length=268, Percent_Identity=27.9850746268657, Blast_Score=104, Evalue=3e-23, Organism=Escherichia coli, GI1787583, Length=275, Percent_Identity=29.0909090909091, Blast_Score=102, Evalue=1e-22, Organism=Escherichia coli, GI87082152, Length=300, Percent_Identity=29, Blast_Score=100, Evalue=7e-22, Organism=Escherichia coli, GI1790299, Length=286, Percent_Identity=29.7202797202797, Blast_Score=96, Evalue=1e-20, Organism=Escherichia coli, GI1788905, Length=229, Percent_Identity=28.82096069869, Blast_Score=91, Evalue=4e-19, Organism=Escherichia coli, GI1786524, Length=411, Percent_Identity=26.0340632603406, Blast_Score=89, Evalue=1e-18, Organism=Escherichia coli, GI87081858, Length=286, Percent_Identity=27.6223776223776, Blast_Score=88, Evalue=2e-18, Organism=Escherichia coli, GI87081872, Length=251, Percent_Identity=27.8884462151394, Blast_Score=87, Evalue=5e-18, Organism=Escherichia coli, GI1789233, Length=263, Percent_Identity=25.4752851711027, Blast_Score=81, Evalue=3e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR003018 - InterPro: IPR020441 - InterPro: IPR009057 - InterPro: IPR002197 - InterPro: IPR002078 [H]
Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]
EC number: NA
Molecular weight: Translated: 94747; Mature: 94615
Theoretical pI: Translated: 8.29; Mature: 8.29
Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00198 4FE4S_FERREDOXIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.1 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 2.1 %Cys (Mature Protein) 1.0 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAAISSETISGIRLFSTLNREQLDRIARHLQLREFAPGQIILSRNEPALELYVILAGRIR CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCEE VELLDEGGQVLTLTELGIGNVIGERAILTDEKRSADVRAITEVQAARLSREDFEELLDQI EEEECCCCCEEEEECCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH PALYANLSRIFAAQLGSWAHRHQREESEHREVITNIIGWQLLPEFGQFPGASHWVRLLNQ HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH RLQQLGGTRSHVLILGEPGTWKDLAARLIHFHSEADRPVLFLDCASPPPVLEEENEPAEG HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCC ASQRGVLLGLAQEAALFGHASQGAVYARRVRRGMIELAAGGDMILRNVDCLALAVQEELV CCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHCHHHHHHHHHHHHH DFLDTGHFTRRGETKLRSARVRIIATSGKALEPLIDSGKFNGELYRKLCGETVELAPLRE HHHHCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHCCCCCHHHHHHHCCCCEEECCHHH RKKDIPVIAKSLLASLNAKHNRNVRRLSQDALNRLVDHDWPLNATELYQVVSRAVVVCSD HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECC TEIQPEHISLQGHPFEDGRFNLLTLPSLERLAWNPRFPKVLRWLTVPAFLLITLYTLLGP CCCCCCEEEECCCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC ASDNAANLAAWTLGWPALILTAFLFARGWCSFCPMEAIGERLGVTSRVVRDPAPWLRSFG CCCCCCCEEEECCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHCCHHHHHCCCHHHHHHCC PSLSFAALVLILLMEQATGMFSHAAATGLLLSGMLTATVSADLVIGRRGWCKFLCPLGRI CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHH VSLVSRISPLEMHSNHNVCLSRCRVDDCIKEKACPMGLHPSGVDSSDHCVLCLNCVRNCP HHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHCCC HHSMQLDLRNPTCGVFNKARRGFREAFFSVTLLGAVIAAKGTPLLAGRQPELFPRTLWTL CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHH EDYLLALCLVAGFTFLALIASVAVRGARWRSVFTSSGLAYLPLAAAGLFLIFFRPLVEGG HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC ARLVPLAVSALGAENFLDATALTPELGTLRLLIYPIILLAALFSWVVLARLQRLDGLPPA CEEHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH ALLRHRLLILGATAILIKIL HHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure AAISSETISGIRLFSTLNREQLDRIARHLQLREFAPGQIILSRNEPALELYVILAGRIR CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCEE VELLDEGGQVLTLTELGIGNVIGERAILTDEKRSADVRAITEVQAARLSREDFEELLDQI EEEECCCCCEEEEECCCCCHHHCCCCEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH PALYANLSRIFAAQLGSWAHRHQREESEHREVITNIIGWQLLPEFGQFPGASHWVRLLNQ HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH RLQQLGGTRSHVLILGEPGTWKDLAARLIHFHSEADRPVLFLDCASPPPVLEEENEPAEG HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCC ASQRGVLLGLAQEAALFGHASQGAVYARRVRRGMIELAAGGDMILRNVDCLALAVQEELV CCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHCHHHHHHHHHHHHH DFLDTGHFTRRGETKLRSARVRIIATSGKALEPLIDSGKFNGELYRKLCGETVELAPLRE HHHHCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHCCCCCHHHHHHHCCCCEEECCHHH RKKDIPVIAKSLLASLNAKHNRNVRRLSQDALNRLVDHDWPLNATELYQVVSRAVVVCSD HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECC TEIQPEHISLQGHPFEDGRFNLLTLPSLERLAWNPRFPKVLRWLTVPAFLLITLYTLLGP CCCCCCEEEECCCCCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC ASDNAANLAAWTLGWPALILTAFLFARGWCSFCPMEAIGERLGVTSRVVRDPAPWLRSFG CCCCCCCEEEECCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHCCHHHHHCCCHHHHHHCC PSLSFAALVLILLMEQATGMFSHAAATGLLLSGMLTATVSADLVIGRRGWCKFLCPLGRI CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHH VSLVSRISPLEMHSNHNVCLSRCRVDDCIKEKACPMGLHPSGVDSSDHCVLCLNCVRNCP HHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHCCC HHSMQLDLRNPTCGVFNKARRGFREAFFSVTLLGAVIAAKGTPLLAGRQPELFPRTLWTL CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHH EDYLLALCLVAGFTFLALIASVAVRGARWRSVFTSSGLAYLPLAAAGLFLIFFRPLVEGG HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC ARLVPLAVSALGAENFLDATALTPELGTLRLLIYPIILLAALFSWVVLARLQRLDGLPPA CEEHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH ALLRHRLLILGATAILIKIL HHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: DNA [C]
Specific reaction: Protein + DNA = Protein-DNA [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2722750 [H]