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The map label for this gene is lipA

Identifier: 197116798

GI number: 197116798

Start: 475972

End: 476847

Strand: Direct

Name: lipA

Synonym: Gbem_0400

Alternate gene names: 197116798

Gene position: 475972-476847 (Clockwise)

Preceding gene: 197116797

Following gene: 197116803

Centisome position: 10.31

GC content: 63.7

Gene sequence:

>876_bases
ATGGATCCGATCAGAAAACCTGCTTGGCTGCAAAAGAAGATCATCCCCGCGGCCCATGCGGAGATGGAGGGGCTTTTAAA
GGAGCTGCGCCTGAACACGGTGTGCCAGCAGGCGCGCTGCCCCAACATCACCGAATGCTTCGGAAAGCGCCAGGCGACCT
TCCTCATCCTCGGGCGCATCTGCACGCGTCTCTGCTCATTTTGTTCCGTCTCCAAGGAGACCCCGCTTCCCTTGGAACCG
GGGGAAGCGGCTTCGGTCGCTGAAGCGGTCAAGCGGCTGGGGCTCTCCCACGTGGTTATTACCAGCCCCACCCGCGACGA
TCTTCCCGACGGCGGCGCCTCCGTCTATGCCGAAACCGTGGCGCGGATCCGGAGCGTTTCCCCTGCGACGAAGGTCGAGC
TGCTGATCCCCGACTTCCGTGGTGATTGGGCCGCCCTGGCCGCCGTGGTCGAGAGCGCGCCCGACATCCTGGGGCATAAC
CTGGAGACGGTGCCGCGGCTTTACTCCATCCGCAGCGGCGCGGACTACAGAAGGTCTCTGGATTTGCTGGCACAGGCGCG
CCGGATGGCCCCCGGCCTCAACACCAAGTCCGGCCTCATGCTGGGCCTCGGGGAAGAGGAAGCGGAACTGTTCGCCGTTA
TGGAAGACCTGCTGAAGGCGGGATGCGGGTACCTGAGCCTGGGGCAATATCTTGCGCCCAGCCGGATGCATCACCCGGTG
CAGCGGTACGTCGAGCCCGAACTGTTCGAACGTTACAAGGAGAGGGCGCTGACGATGGGATTCGAGCACGTCGAGAGCGC
CCCCTACGTTAGAAGCAGCTACCACGCGGAGAATTATCTGGAGTCCAAGTTCCCGCCCCCGGAGGGGGAGGGTTAG

Upstream 100 bases:

>100_bases
ACCTCGAGGGGCGGACTTAGTAGAACTGCAAAAAAGGGGGACAGGCTACTTTTTGAAATGAAAAAGTAGCCTGTCCCCCT
TTTCCAAAGTTGAGGTGCAC

Downstream 100 bases:

>100_bases
GGAGGGGGCAGCTGGACCAAGGGGGACAGGCACCTTGCGGAGCCAGTCCCCCTCCGCCGTTGCAAAGGTTCGTCGAGGAA
CACCGCAGAGGGGTTGTGTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDPIRKPAWLQKKIIPAAHAEMEGLLKELRLNTVCQQARCPNITECFGKRQATFLILGRICTRLCSFCSVSKETPLPLEP
GEAASVAEAVKRLGLSHVVITSPTRDDLPDGGASVYAETVARIRSVSPATKVELLIPDFRGDWAALAAVVESAPDILGHN
LETVPRLYSIRSGADYRRSLDLLAQARRMAPGLNTKSGLMLGLGEEEAELFAVMEDLLKAGCGYLSLGQYLAPSRMHHPV
QRYVEPELFERYKERALTMGFEHVESAPYVRSSYHAENYLESKFPPPEGEG

Sequences:

>Translated_291_residues
MDPIRKPAWLQKKIIPAAHAEMEGLLKELRLNTVCQQARCPNITECFGKRQATFLILGRICTRLCSFCSVSKETPLPLEP
GEAASVAEAVKRLGLSHVVITSPTRDDLPDGGASVYAETVARIRSVSPATKVELLIPDFRGDWAALAAVVESAPDILGHN
LETVPRLYSIRSGADYRRSLDLLAQARRMAPGLNTKSGLMLGLGEEEAELFAVMEDLLKAGCGYLSLGQYLAPSRMHHPV
QRYVEPELFERYKERALTMGFEHVESAPYVRSSYHAENYLESKFPPPEGEG
>Mature_291_residues
MDPIRKPAWLQKKIIPAAHAEMEGLLKELRLNTVCQQARCPNITECFGKRQATFLILGRICTRLCSFCSVSKETPLPLEP
GEAASVAEAVKRLGLSHVVITSPTRDDLPDGGASVYAETVARIRSVSPATKVELLIPDFRGDWAALAAVVESAPDILGHN
LETVPRLYSIRSGADYRRSLDLLAQARRMAPGLNTKSGLMLGLGEEEAELFAVMEDLLKAGCGYLSLGQYLAPSRMHHPV
QRYVEPELFERYKERALTMGFEHVESAPYVRSSYHAENYLESKFPPPEGEG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=40.9090909090909, Blast_Score=226, Evalue=1e-59,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=41.7004048582996, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=48.0286738351254, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=41.4383561643836, Blast_Score=210, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=45.1048951048951, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=41.958041958042, Blast_Score=234, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_GEOBB (B5EAX0)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137225.1
- GeneID:   6783226
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0400
- HOGENOM:   HBG284542
- OMA:   ARCPNIT
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32136; Mature: 32136

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIRKPAWLQKKIIPAAHAEMEGLLKELRLNTVCQQARCPNITECFGKRQATFLILGRI
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHH
CTRLCSFCSVSKETPLPLEPGEAASVAEAVKRLGLSHVVITSPTRDDLPDGGASVYAETV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
ARIRSVSPATKVELLIPDFRGDWAALAAVVESAPDILGHNLETVPRLYSIRSGADYRRSL
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
DLLAQARRMAPGLNTKSGLMLGLGEEEAELFAVMEDLLKAGCGYLSLGQYLAPSRMHHPV
HHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHH
QRYVEPELFERYKERALTMGFEHVESAPYVRSSYHAENYLESKFPPPEGEG
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDPIRKPAWLQKKIIPAAHAEMEGLLKELRLNTVCQQARCPNITECFGKRQATFLILGRI
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHH
CTRLCSFCSVSKETPLPLEPGEAASVAEAVKRLGLSHVVITSPTRDDLPDGGASVYAETV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
ARIRSVSPATKVELLIPDFRGDWAALAAVVESAPDILGHNLETVPRLYSIRSGADYRRSL
HHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHH
DLLAQARRMAPGLNTKSGLMLGLGEEEAELFAVMEDLLKAGCGYLSLGQYLAPSRMHHPV
HHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHH
QRYVEPELFERYKERALTMGFEHVESAPYVRSSYHAENYLESKFPPPEGEG
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA