| Definition | Geobacter bemidjiensis Bem chromosome, complete genome. |
|---|---|
| Accession | NC_011146 |
| Length | 4,615,150 |
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The map label for this gene is polA [H]
Identifier: 197116765
GI number: 197116765
Start: 441203
End: 443881
Strand: Direct
Name: polA [H]
Synonym: Gbem_0365
Alternate gene names: 197116765
Gene position: 441203-443881 (Clockwise)
Preceding gene: 197116764
Following gene: 197116766
Centisome position: 9.56
GC content: 64.43
Gene sequence:
>2679_bases GTGACGACTGAAACAGAGACGCTGTACCTTCTGGACGGCTCTTCCTACATATACCGCGCCTATTTCGCCATCAGGCACCT CTCCTCCCCCAGCGGTTTTCCCACCAACGCGCTCTACGGCTTCACCCAGATGCTTCTTAAGGTGATGAAGGACCGGGCTC CCGCCCACGTCGCCGTCATCTTCGACGCCGGCAAGATCACCTTCAGAAACGAGCTCTTCCCCGCCTACAAGGCGACCAGG AGCGCCATGCCGGAGGACCTGGCCCAGCAGATCGAGCCGATCAAGCAGATGGTGCGCGCCTTCAACATCCCGGCGCTGGA GCTGCCGGGGTTCGAGGCGGACGACATCATCGGGACCATCGCCAAGAAGTGCGAGGCTCAAGGGATGGCCTGCGTCGTTG TGACCGGCGACAAGGACCTGATGCAGATCGTCTCCGACCGGGTCACCCTGCTCGACACCATGAAGGAGAAGAGCTTCGGC ATAGCCGACGTGTACGAGAAGTTCGGGGTGGCGCCGGAGCGTGTGGTGGACGTGCTGGCGCTTTGGGGTGACGCTTCCGA CAACATCCCCGGCGTCCCCGGCATCGGCGAGGTGACGGCCAAGAAGCTCTTGCAGGAATTCGGCTCTCTGGACGAGCTTC TGGCCCGCGCCGGCGAGGTGAAGGGGAAAAACGGCGAGAGGCTCGTGGAGTTCGCCGACCAGGCGCGCCTATGCCGCACC CTTGCCACCATCGACTGCAACGTCCCCATCGAGTACAGCGTCGACGACTTCGCGGTGACTCCGCCGGACAACCGCCGCCT GGCGGCCCTGTTCCGGGAGTACGGCTTCGTGACCCTCTTGAAGGACCTGACCAGCTCTCCCACCCTCTCCTGCGACCGTT ACTCGCTGGTCCTTACCGAGGAGGAGCTGCGCCGCGTGGTGGCCGAGCTGGAGGCGGCCCCCGCCTTCGCCATCGACCTG GAGACCACGAGCCTGAACCCCAGGGAGGCGCGGGTCGTGGGATACGCGGTCAGCTGCCGGCCGCACGAGGCCCGGTACAT CCCGGTCGGGCACCGCTATCTCGGGGCTCCGGAGCAGCTTCCCGAGGCCCTGGTGCTTGAGATCCTCGGGCCGCTTCTGA AGGACCCGGCGCGCCGCAAGATCGGCCAGAACCTGAAGTACGACTACCAGGTGCTCCGGATGGCGGGGTTCGAAATGGAG GGGATCTGGTGCGACACCATGCTGGCCGCCTACCTGGTCAACCCGGCGCGCAACAGCCAGGGGCTGGACGCCCTGGCCTT GGAGTACCTGGACCACCGCATGATCTCGTACGCGGAGGTGGCGGGGAGCGGCAAATGCGAGCTGAACTTCTCCGAGGTGG ATTTGGACCGTGCTGGCCCCTACTCCTGCGAGGACGCCGACGCCACGTATCTCTTGCACGAGATACTGCTCCCCAAGGTG CGGGAGCAGGGGATGGAAGAGCTCCTGTTCGACTTGGAGATGCCGCTGATGCGGATTCTGGCCGATATGGAGCTCCACGG GGTGAAGCTGGACGTTGACCTCATGAAGGAGCTTTCCGCCGGTTTCGGCAAACAGCTGATCGAGCTGGAGGCGCAGATCC ACGAGCACTGCGGCGGCCCCTTCAACATCAACTCCCCCAAACAGCTGGGGGAGATGCTTTTCGAGCGTATGGGGCTTGCC GTCGGGAAAAAAACCAAGGGGAAGACCGGCTGGTCCACCAACGTGGAGGAGCTGGAGCGGCTGGCCGAGGAGCACGAGGT GGCGCGGCTCCTTTTGCAGTACCGAAGCATCTCGAAGCTGAAGTCGACCTATACCGACGCGCTCCCCAAACTGGTCGATC CTGCCACCGGCCGCGTGCACACCTCCTATAACCAGGCGGTGACCAGCACCGGCAGGCTCTCCTCCAGCGACCCCAACCTC CAGAACATACCGATCCGGGGCGAAGAGGGGCGCGGCATCAGGCGCGCCTTCATCGCGGAGCCGGGGAGCCTCATGCTTTC CGCCGATTACTCCCAGATCGAGCTCAGGGTGCTGGCGCACCTCTCCGGCGACCGTGTCCTCTGCGAGGCCTTCGCCGCCG GCGAGGACATCCATCGCCGCACCGCGTCCGAGGTGTTCGGCATGTTCCCGGAGCTGGTGACCTCCGAGATGAGGCGCCAG GCCAAGGTGATCAACTTCGGCGTCATCTACGGCCAGGGGGCCTTCAGCCTCGCCAAGGAGCTGGGGGTGACCCCCAAGCA GGCAAAGGCTTTCATCGACAGCTACTTCGAGCGCCACAGCGGCGCGCGGAGCTTTCTGGACAGCTGCATCAGGGAAGCGG AGGTCTGCGGCTTCGTCACCACCATAATGGGGAGACGGCTCTGCATCCCCGAGATAGCCAGCAAAAACGGCAACGTGCGC GCCTTCGCCCAGAGAAACGCGGTTAACTACCCGATCCAGGGATCGGCCGCCGACATCATCAAGGCAGCCATGCTGAGGGT GATCCGCCGCATGCAGCAGGAGCGGGTGGCGAGCCGGCTCATCATGCAGGTCCACGACGAACTGGTTTTCGAAGTTCCCG AGGCGGAGCGCGATCTCATGGAGGAACTGGTGCGGACCGAGATGGAGGGAGCCCTGCCGCTGTCGGTGCCGCTCAAGGTG GACCTCAATTTCGGGCTTAACTGGAGCGAGGCGCACTAG
Upstream 100 bases:
>100_bases CCCAGAAGGGATGGAAATTCGACGCCCTGCAGTTCGCCATGCGGGACGGAAGCCGCCGCCCCGCCATGGCCTTTGTAGTA TTCACACGCGAGGAGCCCTA
Downstream 100 bases:
>100_bases ATCAGGGAGCGGGGTGGCCAGCGATTAGACTGGTCAGGTTGATATTAAAGGCTTTCCGGAGAGGCGATTATGACCGAGCA GTTGGAGCAGTACCAAGCGC
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 892; Mature: 891
Protein sequence:
>892_residues MTTETETLYLLDGSSYIYRAYFAIRHLSSPSGFPTNALYGFTQMLLKVMKDRAPAHVAVIFDAGKITFRNELFPAYKATR SAMPEDLAQQIEPIKQMVRAFNIPALELPGFEADDIIGTIAKKCEAQGMACVVVTGDKDLMQIVSDRVTLLDTMKEKSFG IADVYEKFGVAPERVVDVLALWGDASDNIPGVPGIGEVTAKKLLQEFGSLDELLARAGEVKGKNGERLVEFADQARLCRT LATIDCNVPIEYSVDDFAVTPPDNRRLAALFREYGFVTLLKDLTSSPTLSCDRYSLVLTEEELRRVVAELEAAPAFAIDL ETTSLNPREARVVGYAVSCRPHEARYIPVGHRYLGAPEQLPEALVLEILGPLLKDPARRKIGQNLKYDYQVLRMAGFEME GIWCDTMLAAYLVNPARNSQGLDALALEYLDHRMISYAEVAGSGKCELNFSEVDLDRAGPYSCEDADATYLLHEILLPKV REQGMEELLFDLEMPLMRILADMELHGVKLDVDLMKELSAGFGKQLIELEAQIHEHCGGPFNINSPKQLGEMLFERMGLA VGKKTKGKTGWSTNVEELERLAEEHEVARLLLQYRSISKLKSTYTDALPKLVDPATGRVHTSYNQAVTSTGRLSSSDPNL QNIPIRGEEGRGIRRAFIAEPGSLMLSADYSQIELRVLAHLSGDRVLCEAFAAGEDIHRRTASEVFGMFPELVTSEMRRQ AKVINFGVIYGQGAFSLAKELGVTPKQAKAFIDSYFERHSGARSFLDSCIREAEVCGFVTTIMGRRLCIPEIASKNGNVR AFAQRNAVNYPIQGSAADIIKAAMLRVIRRMQQERVASRLIMQVHDELVFEVPEAERDLMEELVRTEMEGALPLSVPLKV DLNFGLNWSEAH
Sequences:
>Translated_892_residues MTTETETLYLLDGSSYIYRAYFAIRHLSSPSGFPTNALYGFTQMLLKVMKDRAPAHVAVIFDAGKITFRNELFPAYKATR SAMPEDLAQQIEPIKQMVRAFNIPALELPGFEADDIIGTIAKKCEAQGMACVVVTGDKDLMQIVSDRVTLLDTMKEKSFG IADVYEKFGVAPERVVDVLALWGDASDNIPGVPGIGEVTAKKLLQEFGSLDELLARAGEVKGKNGERLVEFADQARLCRT LATIDCNVPIEYSVDDFAVTPPDNRRLAALFREYGFVTLLKDLTSSPTLSCDRYSLVLTEEELRRVVAELEAAPAFAIDL ETTSLNPREARVVGYAVSCRPHEARYIPVGHRYLGAPEQLPEALVLEILGPLLKDPARRKIGQNLKYDYQVLRMAGFEME GIWCDTMLAAYLVNPARNSQGLDALALEYLDHRMISYAEVAGSGKCELNFSEVDLDRAGPYSCEDADATYLLHEILLPKV REQGMEELLFDLEMPLMRILADMELHGVKLDVDLMKELSAGFGKQLIELEAQIHEHCGGPFNINSPKQLGEMLFERMGLA VGKKTKGKTGWSTNVEELERLAEEHEVARLLLQYRSISKLKSTYTDALPKLVDPATGRVHTSYNQAVTSTGRLSSSDPNL QNIPIRGEEGRGIRRAFIAEPGSLMLSADYSQIELRVLAHLSGDRVLCEAFAAGEDIHRRTASEVFGMFPELVTSEMRRQ AKVINFGVIYGQGAFSLAKELGVTPKQAKAFIDSYFERHSGARSFLDSCIREAEVCGFVTTIMGRRLCIPEIASKNGNVR AFAQRNAVNYPIQGSAADIIKAAMLRVIRRMQQERVASRLIMQVHDELVFEVPEAERDLMEELVRTEMEGALPLSVPLKV DLNFGLNWSEAH >Mature_891_residues TTETETLYLLDGSSYIYRAYFAIRHLSSPSGFPTNALYGFTQMLLKVMKDRAPAHVAVIFDAGKITFRNELFPAYKATRS AMPEDLAQQIEPIKQMVRAFNIPALELPGFEADDIIGTIAKKCEAQGMACVVVTGDKDLMQIVSDRVTLLDTMKEKSFGI ADVYEKFGVAPERVVDVLALWGDASDNIPGVPGIGEVTAKKLLQEFGSLDELLARAGEVKGKNGERLVEFADQARLCRTL ATIDCNVPIEYSVDDFAVTPPDNRRLAALFREYGFVTLLKDLTSSPTLSCDRYSLVLTEEELRRVVAELEAAPAFAIDLE TTSLNPREARVVGYAVSCRPHEARYIPVGHRYLGAPEQLPEALVLEILGPLLKDPARRKIGQNLKYDYQVLRMAGFEMEG IWCDTMLAAYLVNPARNSQGLDALALEYLDHRMISYAEVAGSGKCELNFSEVDLDRAGPYSCEDADATYLLHEILLPKVR EQGMEELLFDLEMPLMRILADMELHGVKLDVDLMKELSAGFGKQLIELEAQIHEHCGGPFNINSPKQLGEMLFERMGLAV GKKTKGKTGWSTNVEELERLAEEHEVARLLLQYRSISKLKSTYTDALPKLVDPATGRVHTSYNQAVTSTGRLSSSDPNLQ NIPIRGEEGRGIRRAFIAEPGSLMLSADYSQIELRVLAHLSGDRVLCEAFAAGEDIHRRTASEVFGMFPELVTSEMRRQA KVINFGVIYGQGAFSLAKELGVTPKQAKAFIDSYFERHSGARSFLDSCIREAEVCGFVTTIMGRRLCIPEIASKNGNVRA FAQRNAVNYPIQGSAADIIKAAMLRVIRRMQQERVASRLIMQVHDELVFEVPEAERDLMEELVRTEMEGALPLSVPLKVD LNFGLNWSEAH
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=540, Percent_Identity=32.962962962963, Blast_Score=254, Evalue=3e-67, Organism=Homo sapiens, GI139394648, Length=687, Percent_Identity=26.783114992722, Blast_Score=221, Evalue=2e-57, Organism=Escherichia coli, GI1790294, Length=921, Percent_Identity=45.385450597177, Blast_Score=778, Evalue=0.0, Organism=Escherichia coli, GI226510970, Length=239, Percent_Identity=28.0334728033473, Blast_Score=106, Evalue=7e-24, Organism=Caenorhabditis elegans, GI86563272, Length=409, Percent_Identity=31.7848410757946, Blast_Score=187, Evalue=2e-47, Organism=Drosophila melanogaster, GI17933644, Length=424, Percent_Identity=31.3679245283019, Blast_Score=198, Evalue=1e-50,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 99144; Mature: 99013
Theoretical pI: Translated: 5.03; Mature: 5.03
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTETETLYLLDGSSYIYRAYFAIRHLSSPSGFPTNALYGFTQMLLKVMKDRAPAHVAVI CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEE FDAGKITFRNELFPAYKATRSAMPEDLAQQIEPIKQMVRAFNIPALELPGFEADDIIGTI EECCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHH AKKCEAQGMACVVVTGDKDLMQIVSDRVTLLDTMKEKSFGIADVYEKFGVAPERVVDVLA HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH LWGDASDNIPGVPGIGEVTAKKLLQEFGSLDELLARAGEVKGKNGERLVEFADQARLCRT HHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH LATIDCNVPIEYSVDDFAVTPPDNRRLAALFREYGFVTLLKDLTSSPTLSCDRYSLVLTE HHHEECCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECH EELRRVVAELEAAPAFAIDLETTSLNPREARVVGYAVSCRPHEARYIPVGHRYLGAPEQL HHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCEEEECCCHHCCCHHHH PEALVLEILGPLLKDPARRKIGQNLKYDYQVLRMAGFEMEGIWCDTMLAAYLVNPARNSQ HHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC GLDALALEYLDHRMISYAEVAGSGKCELNFSEVDLDRAGPYSCEDADATYLLHEILLPKV CHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH REQGMEELLFDLEMPLMRILADMELHGVKLDVDLMKELSAGFGKQLIELEAQIHEHCGGP HHHHHHHHHHHHHHHHHHHHHCCHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCC FNINSPKQLGEMLFERMGLAVGKKTKGKTGWSTNVEELERLAEEHEVARLLLQYRSISKL CCCCCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH KSTYTDALPKLVDPATGRVHTSYNQAVTSTGRLSSSDPNLQNIPIRGEEGRGIRRAFIAE HHHHHHHHHHHCCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC PGSLMLSADYSQIELRVLAHLSGDRVLCEAFAAGEDIHRRTASEVFGMFPELVTSEMRRQ CCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH AKVINFGVIYGQGAFSLAKELGVTPKQAKAFIDSYFERHSGARSFLDSCIREAEVCGFVT HHHEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH TIMGRRLCIPEIASKNGNVRAFAQRNAVNYPIQGSAADIIKAAMLRVIRRMQQERVASRL HHHCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH IMQVHDELVFEVPEAERDLMEELVRTEMEGALPLSVPLKVDLNFGLNWSEAH HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCC >Mature Secondary Structure TTETETLYLLDGSSYIYRAYFAIRHLSSPSGFPTNALYGFTQMLLKVMKDRAPAHVAVI CCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEE FDAGKITFRNELFPAYKATRSAMPEDLAQQIEPIKQMVRAFNIPALELPGFEADDIIGTI EECCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHH AKKCEAQGMACVVVTGDKDLMQIVSDRVTLLDTMKEKSFGIADVYEKFGVAPERVVDVLA HHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH LWGDASDNIPGVPGIGEVTAKKLLQEFGSLDELLARAGEVKGKNGERLVEFADQARLCRT HHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH LATIDCNVPIEYSVDDFAVTPPDNRRLAALFREYGFVTLLKDLTSSPTLSCDRYSLVLTE HHHEECCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECH EELRRVVAELEAAPAFAIDLETTSLNPREARVVGYAVSCRPHEARYIPVGHRYLGAPEQL HHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCEEEECCCHHCCCHHHH PEALVLEILGPLLKDPARRKIGQNLKYDYQVLRMAGFEMEGIWCDTMLAAYLVNPARNSQ HHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC GLDALALEYLDHRMISYAEVAGSGKCELNFSEVDLDRAGPYSCEDADATYLLHEILLPKV CHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH REQGMEELLFDLEMPLMRILADMELHGVKLDVDLMKELSAGFGKQLIELEAQIHEHCGGP HHHHHHHHHHHHHHHHHHHHHCCHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCC FNINSPKQLGEMLFERMGLAVGKKTKGKTGWSTNVEELERLAEEHEVARLLLQYRSISKL CCCCCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH KSTYTDALPKLVDPATGRVHTSYNQAVTSTGRLSSSDPNLQNIPIRGEEGRGIRRAFIAE HHHHHHHHHHHCCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCC PGSLMLSADYSQIELRVLAHLSGDRVLCEAFAAGEDIHRRTASEVFGMFPELVTSEMRRQ CCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH AKVINFGVIYGQGAFSLAKELGVTPKQAKAFIDSYFERHSGARSFLDSCIREAEVCGFVT HHHEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH TIMGRRLCIPEIASKNGNVRAFAQRNAVNYPIQGSAADIIKAAMLRVIRRMQQERVASRL HHHCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH IMQVHDELVFEVPEAERDLMEELVRTEMEGALPLSVPLKVDLNFGLNWSEAH HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]