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The map label for this gene is trmJ [H]

Identifier: 197104457

GI number: 197104457

Start: 1099927

End: 1100712

Strand: Direct

Name: trmJ [H]

Synonym: PHZ_c0991

Alternate gene names: 197104457

Gene position: 1099927-1100712 (Clockwise)

Preceding gene: 197104453

Following gene: 197104458

Centisome position: 27.52

GC content: 71.63

Gene sequence:

>786_bases
ATGTCCGAAGTTTCGTATCCTGCGCCTGTCATTATCCTGAACACGCCCCAGTTGGCGGAAAACATCGGGGCCGTGGCGCG
GGTCATGGCCAACTTCGGCCTGTCGGACCTGCGCCTGGTGAATCCGCGGGACGGCTGGCCCCTTGAACGCGCCTGGGCCT
CGGCTTCCGGCGCGGACTGGCCGCTGAACGCCGCCCGGGTCTACGACAGCGTGGCGGCCGCGATCGCAGACCTGCAGCTC
ATCTATGCGACCACGGCCCGGCCGCGGGAGCTGCAGCTTCCCGTGCTGACGCCCCGCCGGGCGGCGGGCGATCTGCACGC
CGCCGCGGCCGCCGGCCAGCGGGTCGGTCTTCTGTTCGGGGGGGAGCGGGCCGGGCTTGAGACCCAGGACATCGCGCTGT
GCCAGGCGGTGGTGACCATTCCCATCGATCCGCGCTTCCGCTCCCTCAACCTCGCCCAGGCCGTCGCCATCAACGCCTAC
GAGTGGCGGGCGACCGCGGCCGACCGCCCTCCGCCGAACTTCCGCGAGGGGCCGCCGCCGGCCACGGGCGAGGCGCTGCT
GGGCTTCTACGAGCACCTGGAACGCGAACTGGAGACGGCCGGCTTCTTCCACCCGCCGGAGAAGACCCCGAACATGATTC
AGAACCTCCGGAGCGCGCTGGGCCGGGCGCGGTTCACGGACCAGGAGGTCCGCACTCTGCGGGGCGTGGTCACCGCCCTG
TCCCGCGGACGCGGCAAGGTGCTGGAGAAGATCGCCCGCAAGGGCGCGCCCGAGGGCGACGGATGA

Upstream 100 bases:

>100_bases
GCCGGATCCGCAGGCGGCCAGCAGCGTGGCCCCGCCTGCGGCCAGCAGGCCCCGGCGGCAAAGGGTTGCGTTCGTGCGGC
GGGGCTTCTCTAAACGCGGC

Downstream 100 bases:

>100_bases
ACCTGCGCACGCTGCTGGCCGACCTGCCGATCCCGATCCTCCAGGCCCCCATGGTCGGAGCGACGACGGCGGGCATCGCG
CTGGCGGTGAGCCGCGCCGG

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSEVSYPAPVIILNTPQLAENIGAVARVMANFGLSDLRLVNPRDGWPLERAWASASGADWPLNAARVYDSVAAAIADLQL
IYATTARPRELQLPVLTPRRAAGDLHAAAAAGQRVGLLFGGERAGLETQDIALCQAVVTIPIDPRFRSLNLAQAVAINAY
EWRATAADRPPPNFREGPPPATGEALLGFYEHLERELETAGFFHPPEKTPNMIQNLRSALGRARFTDQEVRTLRGVVTAL
SRGRGKVLEKIARKGAPEGDG

Sequences:

>Translated_261_residues
MSEVSYPAPVIILNTPQLAENIGAVARVMANFGLSDLRLVNPRDGWPLERAWASASGADWPLNAARVYDSVAAAIADLQL
IYATTARPRELQLPVLTPRRAAGDLHAAAAAGQRVGLLFGGERAGLETQDIALCQAVVTIPIDPRFRSLNLAQAVAINAY
EWRATAADRPPPNFREGPPPATGEALLGFYEHLERELETAGFFHPPEKTPNMIQNLRSALGRARFTDQEVRTLRGVVTAL
SRGRGKVLEKIARKGAPEGDG
>Mature_260_residues
SEVSYPAPVIILNTPQLAENIGAVARVMANFGLSDLRLVNPRDGWPLERAWASASGADWPLNAARVYDSVAAAIADLQLI
YATTARPRELQLPVLTPRRAAGDLHAAAAAGQRVGLLFGGERAGLETQDIALCQAVVTIPIDPRFRSLNLAQAVAINAYE
WRATAADRPPPNFREGPPPATGEALLGFYEHLERELETAGFFHPPEKTPNMIQNLRSALGRARFTDQEVRTLRGVVTALS
RGRGKVLEKIARKGAPEGDG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=237, Percent_Identity=37.5527426160338, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=33.112582781457, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28157; Mature: 28026

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVSYPAPVIILNTPQLAENIGAVARVMANFGLSDLRLVNPRDGWPLERAWASASGADW
CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCCCC
PLNAARVYDSVAAAIADLQLIYATTARPRELQLPVLTPRRAAGDLHAAAAAGQRVGLLFG
CCHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCHHCCHHHHHHHCCCEEEEEEC
GERAGLETQDIALCQAVVTIPIDPRFRSLNLAQAVAINAYEWRATAADRPPPNFREGPPP
CCCCCCCHHHHHHHHHHEEECCCCCCCCCCHHHHHEECCHHEEECCCCCCCCCCCCCCCC
ATGEALLGFYEHLERELETAGFFHPPEKTPNMIQNLRSALGRARFTDQEVRTLRGVVTAL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SRGRGKVLEKIARKGAPEGDG
HCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEVSYPAPVIILNTPQLAENIGAVARVMANFGLSDLRLVNPRDGWPLERAWASASGADW
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCCCC
PLNAARVYDSVAAAIADLQLIYATTARPRELQLPVLTPRRAAGDLHAAAAAGQRVGLLFG
CCHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCHHCCHHHHHHHCCCEEEEEEC
GERAGLETQDIALCQAVVTIPIDPRFRSLNLAQAVAINAYEWRATAADRPPPNFREGPPP
CCCCCCCHHHHHHHHHHEEECCCCCCCCCCHHHHHEECCHHEEECCCCCCCCCCCCCCCC
ATGEALLGFYEHLERELETAGFFHPPEKTPNMIQNLRSALGRARFTDQEVRTLRGVVTAL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SRGRGKVLEKIARKGAPEGDG
HCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA