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The map label for this gene is rpoA

Identifier: 195952671

GI number: 195952671

Start: 254405

End: 255364

Strand: Direct

Name: rpoA

Synonym: HY04AAS1_0293

Alternate gene names: 195952671

Gene position: 254405-255364 (Clockwise)

Preceding gene: 195952670

Following gene: 195952672

Centisome position: 16.31

GC content: 36.46

Gene sequence:

>960_bases
ATGAAAGAGTTTATTTTTCCGATGAAAATATATTGGGAAGAAAAAGATAAAAATTATGGTAGGTTTGTTGTGGAACCCTT
AGAAAGGGGCTATGGCACTACAATAGGCAATGCTCTTAGAAGGGTTTTACTCTCTTCTATATACGGTAGTGCTATCACCG
CTGTAAAGATAGAAGGTGTTCAACACGAGTTTTCTACTATTGAAGGTGTCCAAGAAGATGTTTTACAGATTATAGCAAAT
CTTAAAAATGTTAGATTTGACTTGAAAGATTCTGATTTAGAGATTTTGTATTTGGAAAAAAATGCGCCAGGTGTTGTTTT
AGCTTCTGATATCAAAACTCCTCCAAATGTCACCATTATAAACAAAGATGCTTACATTGCTACGATAAACTCTGCTAATA
CAACTCTTAAAATGGAAATACGTATAGAAAGAGGCAAAGGTTATGTTATGTCTGATGAAATGGAGCAAATAGGAGAAGCC
GGATGGGTAGTGCTTGATGCTGATTTTTCTCCTATAAAAGTTGCAGCTTTTAGAGTAGAGGCTACTAGAGTGGGTGACAG
AACAGACTATGATAAACTTACTTTCGAGCTTACCACCAACGGTGTTGTATCTCCAGATACGGCTATTCAGCAAGCTGTAG
AGCTTATAGTAAAACATATGAATATGCTTACAAATATATCTTATGAAGTACCAACCTTACCAGAGCCGATGCCTCCCGAC
GAGTTAATGGAAAAGCTTACATTTTCTATAGAAGAGCTTGATATTTCTCAAAGGGCTTTAAACTCGCTGAAAAGGATAGG
GGTAACAACCATAGGAGAACTTGTACAGCTTACAGAGGATGAGCTTAAAAGCTCTAAAAATATAGGCAGAAAAGCTTTGA
CTGAAATAAAAGAAGCTCTTAAAAACATGGGTTTTTCTCTAGGCATGAACATAGGAGAACAAAGAAGTTCAGAGGTGTGA

Upstream 100 bases:

>100_bases
AGAGGTAAGGTTGTGGATAAACCTAAGGATGTTGTTTTGGAAGTGCCTATAAACATGCAGTACATCATTGAGTATTATTC
AAGGGTGTAAGGGGAAAGAT

Downstream 100 bases:

>100_bases
TGTTATGAGACATAGAGTTAAGAAAAAACATTTTAGTAGGACTGCTGAACAAAGGGTTGCTTTGATGAGGTCTTTAGCTA
GGTCTCTTATATTATTTGAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKEFIFPMKIYWEEKDKNYGRFVVEPLERGYGTTIGNALRRVLLSSIYGSAITAVKIEGVQHEFSTIEGVQEDVLQIIAN
LKNVRFDLKDSDLEILYLEKNAPGVVLASDIKTPPNVTIINKDAYIATINSANTTLKMEIRIERGKGYVMSDEMEQIGEA
GWVVLDADFSPIKVAAFRVEATRVGDRTDYDKLTFELTTNGVVSPDTAIQQAVELIVKHMNMLTNISYEVPTLPEPMPPD
ELMEKLTFSIEELDISQRALNSLKRIGVTTIGELVQLTEDELKSSKNIGRKALTEIKEALKNMGFSLGMNIGEQRSSEV

Sequences:

>Translated_319_residues
MKEFIFPMKIYWEEKDKNYGRFVVEPLERGYGTTIGNALRRVLLSSIYGSAITAVKIEGVQHEFSTIEGVQEDVLQIIAN
LKNVRFDLKDSDLEILYLEKNAPGVVLASDIKTPPNVTIINKDAYIATINSANTTLKMEIRIERGKGYVMSDEMEQIGEA
GWVVLDADFSPIKVAAFRVEATRVGDRTDYDKLTFELTTNGVVSPDTAIQQAVELIVKHMNMLTNISYEVPTLPEPMPPD
ELMEKLTFSIEELDISQRALNSLKRIGVTTIGELVQLTEDELKSSKNIGRKALTEIKEALKNMGFSLGMNIGEQRSSEV
>Mature_319_residues
MKEFIFPMKIYWEEKDKNYGRFVVEPLERGYGTTIGNALRRVLLSSIYGSAITAVKIEGVQHEFSTIEGVQEDVLQIIAN
LKNVRFDLKDSDLEILYLEKNAPGVVLASDIKTPPNVTIINKDAYIATINSANTTLKMEIRIERGKGYVMSDEMEQIGEA
GWVVLDADFSPIKVAAFRVEATRVGDRTDYDKLTFELTTNGVVSPDTAIQQAVELIVKHMNMLTNISYEVPTLPEPMPPD
ELMEKLTFSIEELDISQRALNSLKRIGVTTIGELVQLTEDELKSSKNIGRKALTEIKEALKNMGFSLGMNIGEQRSSEV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=316, Percent_Identity=39.873417721519, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_HYDS0 (B4U772)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002120961.1
- ProteinModelPortal:   B4U772
- GeneID:   6743087
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0293
- HOGENOM:   HBG430844
- OMA:   EKPIATI
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35699; Mature: 35699

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFIFPMKIYWEEKDKNYGRFVVEPLERGYGTTIGNALRRVLLSSIYGSAITAVKIEGV
CCCCEEEEEEEEECCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECC
QHEFSTIEGVQEDVLQIIANLKNVRFDLKDSDLEILYLEKNAPGVVLASDIKTPPNVTII
HHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEECCCCCEEEECCCCCCCCEEEE
NKDAYIATINSANTTLKMEIRIERGKGYVMSDEMEQIGEAGWVVLDADFSPIKVAAFRVE
ECCEEEEEEECCCEEEEEEEEEECCCCEEECHHHHHHCCCCEEEEECCCCCEEEEEEEEE
ATRVGDRTDYDKLTFELTTNGVVSPDTAIQQAVELIVKHMNMLTNISYEVPTLPEPMPPD
EHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHH
ELMEKLTFSIEELDISQRALNSLKRIGVTTIGELVQLTEDELKSSKNIGRKALTEIKEAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KNMGFSLGMNIGEQRSSEV
HHCCCEECCCCCCCCCCCC
>Mature Secondary Structure
MKEFIFPMKIYWEEKDKNYGRFVVEPLERGYGTTIGNALRRVLLSSIYGSAITAVKIEGV
CCCCEEEEEEEEECCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECC
QHEFSTIEGVQEDVLQIIANLKNVRFDLKDSDLEILYLEKNAPGVVLASDIKTPPNVTII
HHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEECCCCCEEEECCCCCCCCEEEE
NKDAYIATINSANTTLKMEIRIERGKGYVMSDEMEQIGEAGWVVLDADFSPIKVAAFRVE
ECCEEEEEEECCCEEEEEEEEEECCCCEEECHHHHHHCCCCEEEEECCCCCEEEEEEEEE
ATRVGDRTDYDKLTFELTTNGVVSPDTAIQQAVELIVKHMNMLTNISYEVPTLPEPMPPD
EHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHH
ELMEKLTFSIEELDISQRALNSLKRIGVTTIGELVQLTEDELKSSKNIGRKALTEIKEAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KNMGFSLGMNIGEQRSSEV
HHCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA