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The map label for this gene is thiK [H]

Identifier: 194738197

GI number: 194738197

Start: 1256419

End: 1257243

Strand: Direct

Name: thiK [H]

Synonym: SeSA_A1284

Alternate gene names: 194738197

Gene position: 1256419-1257243 (Clockwise)

Preceding gene: 194737688

Following gene: 194734950

Centisome position: 26.68

GC content: 56.73

Gene sequence:

>825_bases
GTGCGGTCCAACAACAATAATCCCTTAACGCGCGACGAGATCCTGTCGCGCTATTTTCCCCAGTATCGTCCCGCCGTCGC
CGCATCGCAGGGGCTAAGCGGCGGGAGCTGTATTATTGACCACGATACTCACCGTGTCGTGCTGCGTCGTCATCACGACC
CCGACGCTCCACCAGCCCATTTTTTACGTCATTACCGCGCCTTATCCCAATTGCCGGCCAGTCTTGCGCCGCGAGCGCTT
TTTTATACGCCAGGCTGGATGGCGGTAGAATATCTGCATGGTGTGGTAAATTCCGCTCTGCCAGATGCCGACGAACTGGC
GGCCTTACTGTATCATTTGCATCAACAGCCGCGTTTTGGCTGGCGTATCGCGCTATCGCCACTATTGGCGCAGTACTGGT
CGTGTTGCGATCCGGCAAGGCGTACGCCGTTTTGGTTGCGGCGGCTCAAACAGTTGCAAAAAAACGGAGAACCTCGCCCG
CTGCGGCTCGCGCCTTTGCATATGGATGTCCATGGCGACAATATAGTATTAACGTCCGCCGGGTTGAAACTGATTGACTG
GGAGTATGCCGGCGACGGCGATATTGCGTTGGAGCTGGCGGCAGTATGGGTTGAGGATGAACGCCAGCACCGACAACTGG
CAGACGCTTATGCCGCGCGCGCGCGAATCGACGCCCGGCAGCTATGGCGACAGATACGATTATGGCACCCCTGGGTCATT
ATGCTAAAAGCAGGGTGGTTCGAATACCGCTGGCGACAAACCGGCGAGCAACAATTTATCAGGCTGGCCGATGAAACCTG
GCGCCAGCTACGTATGAAAGGATAA

Upstream 100 bases:

>100_bases
TATGTACTTTACTCCAGCGCATCCGGTAACGTTAACGCGCCTGCATTACAGATGCAGTTAATGCTGGTGCAAACGGGCGA
GATAATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
GGAGAGCAGTGTGGGCCCAGTAATGTTGAATGTCGAAGGATGTGAGCTGGATGCGGAGGAGCGCGAGATACTGGCGCATC
CGCTGGTTGGCGGGCTTATT

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIDHDTHRVVLRRHHDPDAPPAHFLRHYRALSQLPASLAPRAL
FYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFGWRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRP
LRLAPLHMDVHGDNIVLTSAGLKLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG

Sequences:

>Translated_274_residues
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIDHDTHRVVLRRHHDPDAPPAHFLRHYRALSQLPASLAPRAL
FYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFGWRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRP
LRLAPLHMDVHGDNIVLTSAGLKLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG
>Mature_274_residues
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIDHDTHRVVLRRHHDPDAPPAHFLRHYRALSQLPASLAPRAL
FYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFGWRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRP
LRLAPLHMDVHGDNIVLTSAGLKLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=273, Percent_Identity=67.032967032967, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 31927; Mature: 31927

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIDHDTHRVVLRRHHDPDAPPAH
CCCCCCCCCCHHHHHHHHCHHHCCHHHHHCCCCCCCEEEECCCCEEHHEECCCCCCCHHH
FLRHYRALSQLPASLAPRALFYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFG
HHHHHHHHHHCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
WRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRPLRLAPLHMDVHGDNIVLTSA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEC
GLKLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
CCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSNNNNPLTRDEILSRYFPQYRPAVAASQGLSGGSCIIDHDTHRVVLRRHHDPDAPPAH
CCCCCCCCCCHHHHHHHHCHHHCCHHHHHCCCCCCCEEEECCCCEEHHEECCCCCCCHHH
FLRHYRALSQLPASLAPRALFYTPGWMAVEYLHGVVNSALPDADELAALLYHLHQQPRFG
HHHHHHHHHHCCHHHCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
WRIALSPLLAQYWSCCDPARRTPFWLRRLKQLQKNGEPRPLRLAPLHMDVHGDNIVLTSA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEC
GLKLIDWEYAGDGDIALELAAVWVEDERQHRQLADAYAARARIDARQLWRQIRLWHPWVI
CCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
MLKAGWFEYRWRQTGEQQFIRLADETWRQLRMKG
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA