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The map label for this gene is sseJ [H]

Identifier: 194738033

GI number: 194738033

Start: 1692014

End: 1693240

Strand: Direct

Name: sseJ [H]

Synonym: SeSA_A1750

Alternate gene names: 194738033

Gene position: 1692014-1693240 (Clockwise)

Preceding gene: 194735215

Following gene: 194734479

Centisome position: 35.93

GC content: 39.28

Gene sequence:

>1227_bases
ATGCCATTGAGTGTTGGACAGGGTTATTTCACATCATCTATCAGTGCTGAAAGATTTAATGTGATAAAAGAAAGCGCACG
CCCTCCGGAATTAAGTTTATGGGAGAAAATCAAAGCATATTTCTTCACCACTTACCATGCCGAGGCGCTCGAATGTATCT
TTAAACTTTACCACTATCAGGAACTGAATCTAACACCGGTACAGGTTCGCGGAGCCTACATCAAACTTCGAGCCTTAGCG
TCTCAGGGATGTAAAGAACAGTTTATTATAGAATCACAGGAACACGCCGATAAGTTGATTATTAAAGATGATAATGGTGA
AAATATTTTATCTATTGAGGTTGAATGTCATCCGGAAGCTTTTGGTCTTGCCAAAGAAATCAATAGATTACATCCCAAGC
CAAAAAATATTTCTTTAGGTGATATTACCAGACTGGTATTTTTTGGCGACAGTTTATCTGACTCCATGGGGCGTATGTTT
GAAAAAACACATCATATCTTACCCTCCTATGGTCAATACTTTGGCGGAAGGTTTACTAATGGATTTACCTGGACTGAGTT
TTTATCATCTCCACACTTCTTAGGTAAAGAGATGCTTAATTTTGCTGAAGGGGGAAGTACATCGGCAAGGTATTCCTGCT
TTAATTGCCTCGGTGACTTTGTATCAAATACGGACAGACAAGTCGCATCTTACACCCCTTCTCACCAGGACCTGGCGATA
TTTTTATTGGGGGCTAATGACTATATGACACTACACAAAGATAATGTAATGATGGTCGTTGAGCAACAAATTGATGATAT
TGAAAAAATAATTTCCGGTGGAGTTAATAATGTTTTAGTCATGGGGATTCCCGATTTGTCTTTGACGCCCTATGGCAAAC
ATTCTGATGAAAAAAGAAAACTTAAGGATGAAAGCACCGCTCACAATGCCCTGCTAAAAACTAATGTTGAAGAATTAAAA
GAAAAATACCCCCAGCACAAAATATGCTATTTCGAGACTGCCGATGCATTTAAGATGATAATGGAGGTGGCCAGTAATAT
TGGTTATGATACGGAAAACCCTTATACTCACCACGGCTATGTACATGTTCCCGGGGCTAAAGACCCTCAGCTGGATATAT
GTCCGCAATACGTCTTCAATGACTTTGTCCATCCAACCCAGGAAGTCCATCATTGTTTTGCCACAATGTTAGAAAGTTTT
ATAGCTCATCATTATTCCACTGAATAA

Upstream 100 bases:

>100_bases
AAGCGCAATTCTATATACACCATTTACCTGATTACTTTTCTTGCTAATATTTGTTAATTAATGTTTTGCTAAAGCGTGAT
TAATTAGTAAGGAGGACACT

Downstream 100 bases:

>100_bases
TGTTCCATCGGCTGCGGTACGCCTTGAGCAAAACACCGCTCAATATCATAGTGCCGGTGGCATGAAAATATGAGGTTGTC
TTTGCTGCCGGTTGGTCAAT

Product: secreted effector protein

Products: NA

Alternate protein names: Salmonella-translocated effector J; STE J [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPLSVGQGYFTSSISAERFNVIKESARPPELSLWEKIKAYFFTTYHAEALECIFKLYHYQELNLTPVQVRGAYIKLRALA
SQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINRLHPKPKNISLGDITRLVFFGDSLSDSMGRMF
EKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSARYSCFNCLGDFVSNTDRQVASYTPSHQDLAI
FLLGANDYMTLHKDNVMMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESTAHNALLKTNVEELK
EKYPQHKICYFETADAFKMIMEVASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDFVHPTQEVHHCFATMLESF
IAHHYSTE

Sequences:

>Translated_408_residues
MPLSVGQGYFTSSISAERFNVIKESARPPELSLWEKIKAYFFTTYHAEALECIFKLYHYQELNLTPVQVRGAYIKLRALA
SQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINRLHPKPKNISLGDITRLVFFGDSLSDSMGRMF
EKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSARYSCFNCLGDFVSNTDRQVASYTPSHQDLAI
FLLGANDYMTLHKDNVMMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESTAHNALLKTNVEELK
EKYPQHKICYFETADAFKMIMEVASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDFVHPTQEVHHCFATMLESF
IAHHYSTE
>Mature_407_residues
PLSVGQGYFTSSISAERFNVIKESARPPELSLWEKIKAYFFTTYHAEALECIFKLYHYQELNLTPVQVRGAYIKLRALAS
QGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINRLHPKPKNISLGDITRLVFFGDSLSDSMGRMFE
KTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSARYSCFNCLGDFVSNTDRQVASYTPSHQDLAIF
LLGANDYMTLHKDNVMMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESTAHNALLKTNVEELKE
KYPQHKICYFETADAFKMIMEVASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDFVHPTQEVHHCFATMLESFI
AHHYSTE

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. H

COG id: COG3240

COG function: function code IR; Phospholipase/lecithinase/hemolysin

Gene ontology:

Cell location: Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from t

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR010637 [H]

Pfam domain/function: PF00657 Lipase_GDSL; PF06767 Sif [H]

EC number: NA

Molecular weight: Translated: 46381; Mature: 46250

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSVGQGYFTSSISAERFNVIKESARPPELSLWEKIKAYFFTTYHAEALECIFKLYHYQ
CCCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEA
CCCCCEEEECCCEEEEEHHHHCCCCHHHCCCCCCCCCEEEEECCCCCEEEEEEEEECCHH
FGLAKEINRLHPKPKNISLGDITRLVFFGDSLSDSMGRMFEKTHHILPSYGQYFGGRFTN
HHHHHHHHHHCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
GFTWTEFLSSPHFLGKEMLNFAEGGSTSARYSCFNCLGDFVSNTDRQVASYTPSHQDLAI
CCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEE
FLLGANDYMTLHKDNVMMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRK
EEECCCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHH
LKDESTAHNALLKTNVEELKEKYPQHKICYFETADAFKMIMEVASNIGYDTENPYTHHGY
HCCHHHHHHHHHHCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEECCCE
VHVPGAKDPQLDICPQYVFNDFVHPTQEVHHCFATMLESFIAHHYSTE
EECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLSVGQGYFTSSISAERFNVIKESARPPELSLWEKIKAYFFTTYHAEALECIFKLYHYQ
CCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEA
CCCCCEEEECCCEEEEEHHHHCCCCHHHCCCCCCCCCEEEEECCCCCEEEEEEEEECCHH
FGLAKEINRLHPKPKNISLGDITRLVFFGDSLSDSMGRMFEKTHHILPSYGQYFGGRFTN
HHHHHHHHHHCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
GFTWTEFLSSPHFLGKEMLNFAEGGSTSARYSCFNCLGDFVSNTDRQVASYTPSHQDLAI
CCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEE
FLLGANDYMTLHKDNVMMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRK
EEECCCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHH
LKDESTAHNALLKTNVEELKEKYPQHKICYFETADAFKMIMEVASNIGYDTENPYTHHGY
HCCHHHHHHHHHHCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEECCCE
VHVPGAKDPQLDICPQYVFNDFVHPTQEVHHCFATMLESFIAHHYSTE
EECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10861017; 11677609 [H]